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The sister chromatids separate after homologous chromosomes because the chromatids are directly conected whereas the homologous chromosomes are not. Crossing over is the process of two non-sister chromatids swapping information.
The mother cellâs DNA is copied during interphase. During mitosis the chromosomes condense from long strands to highly coiled structures. The two copies of each DNA strand, called sister chromatids, are physically attached to one another. The chromosomes are moved to the center of the cell and split apart in a highly coordinated fashion.