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During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. Transcription proceeds in the following general steps: 1  One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA.he antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' â 5'). The complementary RNA is created in the opposite direction, in the 5' â 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine.
There are three stages that make up DNA transcription. First, DNA is transcribed by an enzyme called RNA polymerase. Then, transcription factors elongate the DNA strand and allow RNA polymerase to transcribe only a strand of DNA into a RNA polymer called messenger RNA. Finally, RNA polymerase continues along the DNA until it reaches what is called a terminator sequence.