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During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. Transcription proceeds in the following general steps: 1  One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA.he antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' â 5'). The complementary RNA is created in the opposite direction, in the 5' â 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine.
Although transcription is performed by RNA Polymerase, the enzyme needs other proteins to produce the transcript. These factors are either associated directly with RNA Polymerase or add in building the actual transcription apparatus. The general term for these associated proteins is transcription factor.he definition is reflects the interaction of the all of the important proteins and the DNA to which the bind. Promoter-all the DNA sequences containing binding sites for RNA polymerase and the transcription factors necessary for normal transcription Copyright Â© 1998.