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The sister chromatids separate after homologous chromosomes because the chromatids are directly conected whereas the homologous chromosomes are not. Crossing over is the process of two non-sister chromatids swapping information.
â¦at a point called the centromere. During mitosis the sister chromatids separate, one going to each daughter cell. Chromosomes thus meet the first criterion for being the repository of genes: they are replicated, and a full copy is passed to each daughter cell during mitosis.