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The sister chromatids separate after homologous chromosomes because the chromatids are directly conected whereas the homologous chromosomes are not. Crossing over is the process of two non-sister chromatids swapping information.
The separation of homologous chromosomes allows for each homologue to reach each gamete. This enables each daughter cell to have only 23 chromosomes, which is one from each homologous pair. Sister chromatids remain intact during meiosis I but split during meiosis II. After crossing over occurs, the chromatids separate during anaphase II and line up along the equator during metaphase I of meiosis I. Learn more about Biology