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The sister chromatids separate after homologous chromosomes because the chromatids are directly conected whereas the homologous chromosomes are not. Crossing over is the process of two non-sister chromatids swapping information.
What forms to help attach and move chromatids to the opposite poles of the cells? The mitotic spindle fibers formed of tubulin subunits. What is the cell structure that joins two sister chromatids of a chromosome? The cell structure that joins two sister chromatids into one single  chromosome is called the centromere. This is at the center of the  sister chromatids.