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During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. Transcription proceeds in the following general steps: 1  One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA.he antisense strand of DNA is read by RNA polymerase from the 3' end to the 5' end during transcription (3' â 5'). The complementary RNA is created in the opposite direction, in the 5' â 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine.
Transcription & Translation: Transcription (Advanced). Transcription is the process by which the information in DNA is copied into messenger RNA (mRNA) for protein production. Transcription begins with a bundle of factors assembling at the promoter sequence on the DNA (in red).Here, two transcription factors are already bound to the promoter.ther proteins arrive, carrying the enzyme RNA polymerase (in blue). To initiate transcription, these assembled proteins require contact with activator proteins that bind to specific sequences of DNA known as enhancer regions. Once the contact is made, the RNA polymerase races along the DNA to transcribe the gene.