Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
1. Know When You Ovulate after Childbirth. Most women experience an extended period of bleeding immediately after giving birth. The bleeding will get lighter and lighter, lasting about six weeks. It is extremely unlikely that a woman will become pregnant during this time, although it is not impossible.
If you have a pregnancy within six month of giving birth, you also increase the risks for your pregnancy having complications. While the outcomes are slightly better if you wait between 6 months and 18 months, the pregnancies that do the best are those where the mother has at least 18 months since she last gave birth.