This handout is based on the 6th edition of the Publication Manual of the American Psychological. Association (APA), but is not a comprehensive guide. For all rules and requirements of APA. citations, please consult the 6th edition of the Publication Manual of the American Psychological. Association. APA requires that information be cited in 2 different waysâwithin the text and in a reference list at. the end of the paper.
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This will amplify a 500 bp product at least 105-fold in 25 cycles, taking about 2.3 minutes per cycle. DNA denaturation is the critical step in the GeneAmpÂ® PCR process and is often the focus of attention if PCR experiments fail. The practical range of denaturation temperatures for most samples is 94 Â°C-96 Â°C.