Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
These dyes are not always stable in alkaline formulations and may fade in soaps. Please test dyes in your products to see if they will remain stable over time. Using either our liquid dye or the powdered dye diluted in distilled water, add the dye to your water phase drop by drop until you get the desired color. When adding dyes to dry salts or powders, you will need to be patient while mixing as it takes awhile, but it will color uniformly.