Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Such dyeing consists of two stages: soaking the fiber material in the dye solution and fixation in a basic medium. In the fixation stage, the dye reacts with the fiber to form a new coloring compoundâfor example, Part of the dye (up to 30 percent) reacts with water.