Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Soak the material to be dyed in the dye fixer solution. Let the fabric soak in the solution for 5 to 10 minutes, or until the fabric is completely saturated. You can reuse the fixer water and treat several batches of fabric in the same mixture. Step 4: Fold, Twist or Tie.