Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
1. Know When You Ovulate after Childbirth. Most women experience an extended period of bleeding immediately after giving birth. The bleeding will get lighter and lighter, lasting about six weeks. It is extremely unlikely that a woman will become pregnant during this time, although it is not impossible.
Depo-Provera can affect fertility for several months. It depends on how long you were on it and even then every woman is different. Most women are able to get pregnant in the year following the cessation of their injections. Some can become pregnant right away and some it takes up to 18 months. You do not have to have regular periods to get pregnant.