Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
The intercalating dyes used in qPCR fluoresce only when they are bound to double-stranded DNA (dsDNA). They do not fluoresce in the presence of single-stranded DNA (ssDNA), or when the dyes are free in solution.