Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
1 EDIT: You want the dye to be active otherwise the dye will come out in the rinse water. So heat to just below boiling and hold it there.  wind your yarn into a non-center pull ball, do not pre-wet the yarn.