By Dr Ananya Mandal, MD. Leptin is a protein hormone involved in the regulation of energy intake and expenditure by the body. Leptin is an important cytokine that plays a key role in the regulation of appetite, food intake and metabolism.y Dr Ananya Mandal, MD. Leptin is a protein hormone involved in the regulation of energy intake and expenditure by the body. Leptin is an important cytokine that plays a key role in the regulation of appetite, food intake and metabolism.
Report Abuse. PCR The polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication.As PCR progresses, the DNA thus generated is itself used as template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified.With PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece.ith PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece.
385 EXPERIMENT 24 Amplification of a DNA Fragment Using Polymerase Chain Reaction Theory Polymerase chain reaction (PCR) is a techniquethat allows the amplification of a specific fragmentof double-stranded DNA in a matter of hours.ny biological sample recovered from a crime scenethat contains DNA (a single hair, a drop of blood,skin cell, saliva, etc.) can be subjected to PCR.Primers complementary to repetitive DNA se-quences found throughout the human genome arechange a single base pair in the gene of interest(Fig. 24-4 d).