The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
For instance, if you get a torrential downpour 6â gutters will get the water where it needs to go whereas 5â gutters might overflow. The average cost of 6â gutters ranges between $1,000-$1500 for the same, 2,000 square foot home.Average cost of gutters per foot for 5â gutters is between 5-8 dollars and for 6â gutters between 8-10 dollars. 5â seamless: $800-$1200.5â cost per foot: 5-8 dollars.ereâs a cost breakdown for a standard 2,000 square foot home: For 5â seamless gutters on a typical home (2,000 square foot raised ranch or cape) the average price of a gutter installation ranges from $800-$1200. However with more unpredictable weather, many homeowners get a free estimate for 6â gutters.