Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
1. Know When You Ovulate after Childbirth. Most women experience an extended period of bleeding immediately after giving birth. The bleeding will get lighter and lighter, lasting about six weeks. It is extremely unlikely that a woman will become pregnant during this time, although it is not impossible.
A womanâs likelihood of getting pregnant can rise and fall throughout her ovulation cycle. While the average femaleâs monthly cycle may be 29 days, others may have a cycle that varies from 20 to 40 days, or longer. The likelihood that a woman will get pregnant one to two days after she starts bleeding is nearly zero. But the likelihood starts to increase again with each successive day, even though sheâs still bleeding. At roughly day 13 after starting her period, her chance of pregnancy is an estimated 9 percent.