Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Pour dye colors over yarn leaving space between each color. Using a rod, poke yarn so dye moves to cover white spaces between colors. The dye will strike quickly because of the heat. Be careful not to overstir because you do not want the colors to migrate any more than necessary to cover the undyed areas of yarn.