The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
How Much Does Gutter Cleaning Cost? When you are ready to hire a gutter cleaner to remove the dead leaves and debris from your gutters you need to find a price that works with your budget. Research indicates that the average cost homeowners spent on gutter cleaning in 2012 is $182. Most spent between $162 and $202 but the price for a cleaning job can vary. The cost to clean residential and commercial gutters is determined by several factors. Knowing what these factors are and how your home measures up will help you prepare so you can find a gutter cleaner you can afford.