Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
One thing that helps to have less dye come off and less fading in the future, is after one brief rinse, to put the fabric in a hot soak with Dharma Dye Fixative or Retayne for 1/2 hour (1 oz fixative per lb. of fabric). This causes more of the Indigo to stick to the fabric.ne thing that helps to have less dye come off and less fading in the future, is after one brief rinse, to put the fabric in a hot soak with Dharma Dye Fixative or Retayne for 1/2 hour (1 oz fixative per lb. of fabric). This causes more of the Indigo to stick to the fabric.