Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Dye crocking is the result of color loss by mechanical actions such as friction and abrasion. Crocking can be the result of lack of penetration of the dyeing agent, the use of incorrect dyes or dyeing procedures, or the lack of proper washing procedures and finishing treatments after the dyeing process.