Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Step 4: Let the dye sit. Once your shirts are all dyed, put them in plastic bags to let the dyes set. You want them to remain damp, so sealing them in ziplock bags works well. The kit I had said to leave them 6-8 hours, but I left them overnight and found that that made the colors much brighter.