Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
1. Know When You Ovulate after Childbirth. Most women experience an extended period of bleeding immediately after giving birth. The bleeding will get lighter and lighter, lasting about six weeks. It is extremely unlikely that a woman will become pregnant during this time, although it is not impossible.
There's been a lot of controversy about how long women should wait to try to get pregnant again after a miscarriage. Some doctors suggest waiting three or even six months to normalize hormone levels after a pregnancy loss, but this advice is largely theoretical and not based on scientific studies.