Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
1. Know When You Ovulate after Childbirth. Most women experience an extended period of bleeding immediately after giving birth. The bleeding will get lighter and lighter, lasting about six weeks. It is extremely unlikely that a woman will become pregnant during this time, although it is not impossible.
After an unprotected sex, how long will it take till you know you are pregnant? You are most likely to get pregnant if you had sex around the time of your ovulation. This is when you are fertile because an egg has just been released. This happens about two weeks from the first day of your last menstruation.