The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
The UK's Most Comprehensive Building Cost/Price Guide. Average Labour Cost/Price to Clean Out Gutters/Guttering. (These prices are based on a tradesmanâs rate of Â£150.00 per day and a labourer if required at Â£100.00 per day. This includes the cost of buying and collecting any materials, dumping any waste if necessary and any incidental materials they will need.