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Probably limited a small bit by structural strength, i.e. more acceleration means a stronger and heavier structure. For manned rockets, the G forces are even lower to accomodate the astronauts. So I'm assuming that even lower accelerations are only possible if you trade off fuel efficiency.
Therefore, astronauts do regular exercise and strength training. before, during, and after a mission to keep their muscles and bones strong. Performing multi-joint weight-bearing exercises, such as the push-up for upper body strength and the. squat for lower body strength, can help develop stronger muscles and bones.