The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
Typical Gutter Cleaning Costs. 1  Single-story home: A typical 1,500-square-foot home can cost $65-$225 to clean the gutters. 2  Two-story home: A 2,500-square-foot home with about 200 linear feet of gutters will cost $100-$300 to clean. 3  Hourly: The average cost of gutter cleaning is about $65 per hour.t least once a year, your gutters need to be cleared of leaves, sticks, and debris to make sure runoff isn't causing any major damage to your roof. Single-story and smaller two-story homes: About $65.