The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
Cost breakdown. For the gutter guard installation, the typical costs include: 1  Materials-costs on gutter guards of all kinds range quite widely with some being as little as $3 for a three foot section and some climbing as high as $18 to $20 per single foot.he standard cost for a professional to clean them is around $75 per cleaning, which means maintenance is $225 per year. The average DIY home or property owner can easily install gutter guards and also tackle the annual cleanings quite a bit easier too.