The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
The price of professional gutter cleaning can seem like a moving target sometimes. The range of prices can be attributed to time of year, other work a contractor may be doing, overall demand for gutter cleaning services and other factors.However, if your gutters are overflowing itâs probably a good time to reach out to a professional and get your gutters and downspouts cleaned.or a typical single story home of 1,500 square feet, cleaning can run between $65 and $250. For a two story home in the 2,500 square foot range the price goes up to $125 to $300 for a normal cleaning.