By Dr Ananya Mandal, MD. Leptin is a protein hormone involved in the regulation of energy intake and expenditure by the body. Leptin is an important cytokine that plays a key role in the regulation of appetite, food intake and metabolism.y Dr Ananya Mandal, MD. Leptin is a protein hormone involved in the regulation of energy intake and expenditure by the body. Leptin is an important cytokine that plays a key role in the regulation of appetite, food intake and metabolism.
Report Abuse. PCR The polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication.As PCR progresses, the DNA thus generated is itself used as template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified.With PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece.ith PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece.
The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR).The method is so-called because the amplification of DNA with a polymerase chain reaction (PCR) is monitored in real time (qPCR cyclers constantly scan qPCR plates).ow it works. PCR is a method where an enzyme (thermostable DNA polymerase, originally isolated in 1960s from bacterium Thermus aquaticus, growing in hot lakes of Yellowstone park, USA) amplifies a short specific part of the template DNA (amplicon) in cycles.