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Equally important and coupled with DNA methylation is DNA demethylation, the removal of a methyl group. The demethylation process is necessary for epigenetic reprogramming of genes and is also directly involved in many important disease mechanisms such as tumor progression.hen a CpG island in the promoter region of a gene is methylated, expression of the gene is repressed (it is turned off). The addition of methyl groups is controlled at several different levels in cells and is carried out by a family of enzymes called DNA methyltransferases (DNMTs).
The hypothesis on the involvement of DNA methylation in the repair process in eukaryotes was disproved by Araujo et al [7]. It was shown that methylation in eukaryotic cells occurs immediately after replication and even Okazaki fragments are already methylated [7].nother mechanism of transcription regulation by methylation of single sites involves methylation dependent binding proteins (MDBPs). MeCP1 described by Meehan et al. needs at least 7 methylated CpGs for efficient DNA binding and therefore is less important for genes without CpG islands in the promoters [ 35 ].