The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
1 Cost for a single-story 2,000-square foot home with around 180 linear feet of gutter is about $90-$225. clean gutter is designed to draw water away from a home's foundation, according to Do It Yourself.com. When a gutter overflows with debris, it may also cause roof damage.A gutter cleaning service may be priced by the square footage of the home, or the linear foot of gutter.