The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes. These are removed by the 5' to 3' exonuclease action of Polymerase I. 2  5. 3  The final product does not have any gaps in the DNA that result from the removal of the RNA primer. 4  These are filled in by the action of DNA Polymerase I.
The cost to clean gutters usually ranges from $101 to $189, but prices could be as low as $60 for small jobs or as much as $310 for big jobs. Using gutter guards and regular maintenance are ways to reduce these costs.
Related articles: Gutters, Roof Repair, Window Cleaning. 1  Cleaning the gutters on a single-story 1,500-square foot house with about 160 linear feet of gutter will run $70-$200. 2  Cost for a single-story 2,000-square foot home with around 180 linear feet of gutter is about $90-$225. For three-story homes, or anywhere a 40-foot ladder is used, a second worker is required. 2  Add about $75-$200 to the price. 3  Downspouts can take extra time and may bring the total cost up to $500 if seriously clogged.