This handout is based on the 6th edition of the Publication Manual of the American Psychological. Association (APA), but is not a comprehensive guide. For all rules and requirements of APA. citations, please consult the 6th edition of the Publication Manual of the American Psychological. Association. APA requires that information be cited in 2 different waysâwithin the text and in a reference list at. the end of the paper.
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A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR.uantifying gene expression by traditional DNA detection methods is unreliable. Detection of mRNA on a Northern blot or PCR products on a gel or Southern blot does not allow precise quantification.