Some examples of substrates used (also known as chromogens) are AEC (3-Amino-9-EthylCarbazole), or DAB (3,3'-Diaminobenzidine). Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.
1. Know When You Ovulate after Childbirth. Most women experience an extended period of bleeding immediately after giving birth. The bleeding will get lighter and lighter, lasting about six weeks. It is extremely unlikely that a woman will become pregnant during this time, although it is not impossible.
for as long as you are breasfeeding, you will not have ovulation so you wil not have your menstruation but if no, you will have your period as early as 3-4 months after giving birth.