o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
Every ticket you receive in Michigan will remain on your record for two years from the date of your conviction. If you pay the ticket, this is two years from the date you pay. If you contest the ticket, it is two years from the date you are convicted in traffic court.