Replication of TBEV and distribution of virus antigen in human neurons. We employed a plaque assay to determine TBEV infection and replication kinetics in human neurons (HNs; Fig. 1A). The HNs were infected with TBEV, and at 0, 3, 5, 7, and 12 days post infection (p.i.), cell supernatants were collected.
Daymond John. Daymond Garfield John (born February 23, 1969) is an American businessman, investor, television personality, author and motivational speaker. He is best known as the founder, president, and CEO of FUBU, and appears as an investor on the ABC reality television series Shark Tank. He is currently based in New York City.
This is how FUBU was born. At the beginning they started sewing the FUBU logo on t-shirts, jackets and other clothes trying to make the brand popular. This didnât have a great effect and the business wasnât really booming. John had to work full-time as a waiter in Red Lobster restaurant.