o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
South Dakota doesn't assess points for speeding. Minnesota (171.12(6)) and Arizona do not have points for speeding in some 55 zones. Ohio law prohibit points for speeding 10 miles per hour or less over a limit of 55 or higher, or 5 miles per hour or less over a speed limit below 55.