Replication of TBEV and distribution of virus antigen in human neurons. We employed a plaque assay to determine TBEV infection and replication kinetics in human neurons (HNs; Fig. 1A). The HNs were infected with TBEV, and at 0, 3, 5, 7, and 12 days post infection (p.i.), cell supernatants were collected.
Daymond John. Daymond Garfield John (born February 23, 1969) is an American businessman, investor, television personality, author and motivational speaker. He is best known as the founder, president, and CEO of FUBU, and appears as an investor on the ABC reality television series Shark Tank. He is currently based in New York City.
Bupa (originally, the British United Provident Association) was established in 1947 when 17 British provident associations joined together to provide healthcare for the general public. The firm is a private company limited by guarantee; it has no shareholders, and any profits (after tax) are reinvested in the business.