o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
A $145 speeding ticket for going 15 mph over the speed limit in Minnesota is, on average, 3.59 times more costly than that dollar amount on the citation.reaking it down by city, Nerdwallet found a Prior Lake driver will end up paying $670.75 for a speeding ticket after the insurance premiums are factored in â the highest average cost in the state. The second-highest, Lakeville, comes in at $666.25, with No. 3 Cottage Grove at $592.87. Hereâs the top 10 chart: