o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
The short answer is that the accident or ticket will stay on your record for three years, but the exact punishments you receive for your traffic violation depend on several factors, including: 1  Your insurance company. 2  How long you have been with your provider. 3  Where you live. 4  How old you are. 5  The severity of the accident or violation.