o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
Tickets will remain on your Illinois Secretary of State driver's abstract for a period between 4 1/2 and 7 years. The tickets will be removed once the time period passes. The nature of the ticket will determine how long it will stay on. You can contact the Illinois Secretary of State and ask them to review your record to determine if any tickets can be removed.