Replication of TBEV and distribution of virus antigen in human neurons. We employed a plaque assay to determine TBEV infection and replication kinetics in human neurons (HNs; Fig. 1A). The HNs were infected with TBEV, and at 0, 3, 5, 7, and 12 days post infection (p.i.), cell supernatants were collected.
Daymond John. Daymond Garfield John (born February 23, 1969) is an American businessman, investor, television personality, author and motivational speaker. He is best known as the founder, president, and CEO of FUBU, and appears as an investor on the ABC reality television series Shark Tank. He is currently based in New York City.
John started off by making and selling wool hats with his friend Carl Brown. He worked at Red lobster in the evenings and made his hats at night. He said one thing that he sacrificed a lot when he was starting FUBU was his sleep. Once the business had gained traction, John tried and failed to seek funding to expand the business, being turned down by 27 banks. But he did not give up and so he mortgaged his home for $100,000.