Replication of TBEV and distribution of virus antigen in human neurons. We employed a plaque assay to determine TBEV infection and replication kinetics in human neurons (HNs; Fig. 1A). The HNs were infected with TBEV, and at 0, 3, 5, 7, and 12 days post infection (p.i.), cell supernatants were collected.
Daymond John. Daymond Garfield John (born February 23, 1969) is an American businessman, investor, television personality, author and motivational speaker. He is best known as the founder, president, and CEO of FUBU, and appears as an investor on the ABC reality television series Shark Tank. He is currently based in New York City.
Haibun (ä¿³æ, literally, haikai writings) is a prosimetric literary form originating in Japan, combining prose and haiku.he first contest for English-language haibun took place in 1996, organized by poet and editor Michael Dylan Welch, and judged by Tom Lynch and Cor van den Heuvel. Anita Virgil won first prize, and David Cobb won second prize. The contest resulted in the publication of Wedge of Light (Press Here) in 1999.