Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
The current Premier is Mike Baird, the Leader of the New South Wales Liberal Party. His Deputy is Andrew Stoner, the Leader of the New South Wales National Party. Baird replaced Barry O'Farrell on 17 April 2014, after O'Farrell stood down as Premier.