In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Titanium by David Guetta - (v1) Titanium is a song by French disc jockey and record producer David Guetta from his upcoming fifth studio album, Nothing but the Beat. It features vocals from Australian pop singer Sia Furler. The song was released digitally on August 5, 2011 as the first of three promotional singles from the album.