In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
In 1955, he wrote two hit songs: 'Vecchio Frao', the drama of a man who no longer has the strength to live and 'Lu Pisci Spada', a song about blood, love and death in Sicilian dialect. In 1957, he wrote three other classics: 'Resta cu'mme', 'Lazarella' and 'Strada anfosa'.