In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Tozzi's Gloria is a love song, as is the first English rendering of the song, recorded by its original English-language lyricist, Jonathan King, in November 1979; Tozzi later recorded and performed King's translated version of Gloria.