In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Titanium is a song produced and co written by David Guetta and performed by Sia Furler. Titanium has a very pop, techno type of sound, but it is meant to be a ballad. The music and beats associated with this song will often take away the true meaning that the lyrics actually possess.