In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
+25 other releases. read more. Titanium is a song recorded by French disc jockey (DJ)-music producer David Guetta, featuring vocals by Australian recording artist Sia. Taken from the former's fifth studio album, Nothing but the Beat, the song was written by Sia Furler, Guetta, Giorgio Tuinfort and Afrojack. Production was also handled by Guetta, Tuinfort and Afrojack. Titanium was initially released for digital download on August 8, 2011, as theâ¦.