In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
The same year saw Fragma still operating with vocalist Damae, and releasing a second album; Embrace, with hit singles such as Say That You're Here, Embrace Me, Man In The Moon and Time And Time Again. Dirk and Marco are also currently working as a duo under their surname Duderstadt.