In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Titanium by David Guetta. This is a promotional single off David Guetta's fifth studio album Nothing But The Beat. The single features Australian pop singer-songwriter Sia Furler and was released on August 8, 2011. Guetta told Artist Direct about this track: That song is interesting because it's different from anything else on the album.