Mispriming in PBAT libraries causes methylation bias and poor mapping efficiencies. Random priming in PBAT libraries introduces drastic biases in the base composition and methylation levels especially at the 5â² end of all reads. As a result, affected bases should be removed from the libraries before the alignment step.
Mariano Rajoy Brey, President of the Government of Spain. Property Registrar. Vice-Chairman of the Governing Council of Alianza Popular [People's Alliance] in Galicia. Chairman of the Local and Provincial Council of Alianza Popular [People's Alliance] in Pontevedra. Member of the Permanent Committee of Alianza Popular [People's Alliance].
The party in power right now (2013) is the Democrats. Barack Obama is the leader of the Democrats. And as a result, the Republicans are the opposition partâ¦y. Conversely, in 2000, George W. Bush got elected president. He was a Republican and thus, the leader of the Republican party. Because a Republican was president, it was now the Democrats who were the opposition party. So, the leader of the opposition is the man or woman who is considered to be in charge of the political party that is NOT in power.