In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Words & Music by George Weiss / Hugo Peretti / Luigi Creatore. 1  Wise men say only fools rush in. but I can't help falling in love with you. 2  Like a river flows surely to the sea. Darling so it goes. 3  Like a river flows surely to the sea. Darling so it goes. 4  Recorded: 1960/03/23, first released on Blue Hawaii.