In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase.
Little Richard's Tutti Frutti (1955) was included in the National Recording Registry of the Library of Congress in 2010, which stated that his unique vocalizing over the irresistible beat announced a new era in music..
Donât bother, says Perry. The beauty of a frittata is that it serves a crowd and keeps well. Use too much dairy, and the eggs will be too loose. Use too little, and youâll miss out on the creamy-luscious goodness.