Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together. The DNA to be cloned can vary widely, from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one vector to another (subcloning).
Weather. Melbourne enjoys warm summers, glorious springs, mild autumns and crisp winters. With its variable climate, Melbourne hots up December to February (summer), cools down March to May (autumn), chills out June to August (winter), and warms up again September to November (spring). Melbourne's top temperatures are usually in January and February. Those months are often dry, with hot spells and cooling afternoon breezes off Port Phillip Bay. June and July are the coldest months, and October is the wettest.
Flights to Melbourne, Australia. With a zest for events, dining and shopping, Melbourne is a city where the best attractions are the ones you discover for yourself. A city of hidden laneways and unassuming shopping malls, rooftop bars, graffiti art and.hole-in-the-wall cafes, Melbourne lends itself to getting lost.irlines that fly to Melbourne. 1  British Airways. 2  Cathay Pacific. 3  Emirates. 4  Etihad Airways. 5  Malaysia Airlines. 6  QANTAS Airlines. 7  Qatar Airways. 8  Royal Brunei.