Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together. The DNA to be cloned can vary widely, from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one vector to another (subcloning).
Weather. Melbourne enjoys warm summers, glorious springs, mild autumns and crisp winters. With its variable climate, Melbourne hots up December to February (summer), cools down March to May (autumn), chills out June to August (winter), and warms up again September to November (spring). Melbourne's top temperatures are usually in January and February. Those months are often dry, with hot spells and cooling afternoon breezes off Port Phillip Bay. June and July are the coldest months, and October is the wettest.
Temperatures average 25Â°C in summer and 14Â°C in winter. Rainfall is highest from May to October. Here you will find some information on temperature, rainfall and seasonal activities to help you plan your holiday in Melbourne.eather in Australia. Weather in Melbourne. Victoria's climate is marked by a range of different climate zones, from the hot, dry regions of the northwest to the alpine snowfields in the northeast. Melbourne has a reputation for its changeable weather, often referred to as having âfour seasons in one day'.