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The most common vector used in genetic engineering is a circular form of DNA known as a plasmid. Endonucleases are used to cut the plasmid molecule open at almost any point chosen by the scientist. Once the plasmid has been cut open, it is mixed with the insulin gene and a ligase enzyme.
GMOs, or genetically modified organisms are organisms whose genetic material has been altered using genetic engineering. Genetic engineering is the modification of an organism's phenotype by altering its genetic make-up. Genetic engineering is primarily performed by simple mating or gene recombination.grobacterium is bacteria that uses a Horizontal gene transfer (HGT). HGT is the transfer of DNA between different genomes [Pop up: A genome is the complete set of genetic material present in an organism].