Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together. The DNA to be cloned can vary widely, from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one vector to another (subcloning).
Weather. Melbourne enjoys warm summers, glorious springs, mild autumns and crisp winters. With its variable climate, Melbourne hots up December to February (summer), cools down March to May (autumn), chills out June to August (winter), and warms up again September to November (spring). Melbourne's top temperatures are usually in January and February. Those months are often dry, with hot spells and cooling afternoon breezes off Port Phillip Bay. June and July are the coldest months, and October is the wettest.
This report describes the typical weather at the Melbourne Airport (Melbourne, Australia) weather station over the course of an average September. It is based on the historical records from 1986 to 2012. Earlier records are either unavailable or unreliable.Melbourne has a mild humid temperate climate with warm summers and no dry season. The area within 25 mi of this station is covered by croplands (50%), built-up areas (18%), forests (17%), grasslands (8%), and oceans and seas (8%).arlier records are either unavailable or unreliable. Melbourne has a mild humid temperate climate with warm summers and no dry season. The area within 25 mi of this station is covered by croplands (50%), built-up areas (18%), forests (17%), grasslands (8%), and oceans and seas (8%).