Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together. The DNA to be cloned can vary widely, from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one vector to another (subcloning).
Weather. Melbourne enjoys warm summers, glorious springs, mild autumns and crisp winters. With its variable climate, Melbourne hots up December to February (summer), cools down March to May (autumn), chills out June to August (winter), and warms up again September to November (spring). Melbourne's top temperatures are usually in January and February. Those months are often dry, with hot spells and cooling afternoon breezes off Port Phillip Bay. June and July are the coldest months, and October is the wettest.
Peak season: Melbourne is busiest in summer and winter but you can always find last minute cheap flight to Melbourne. The Australian Open is last two weeks of January, and the Australian Formula One Grand Prix is held in early March.elbourne climate. In the summer, Melbourne temperatures can reach 40Â°C or higher. Spring and autumn weather is highly changeable. Spring is the best time to visit â but remember, Melbourne's spring is from September to November so make sure you book flights to Melbourne accordingly.