Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together. The DNA to be cloned can vary widely, from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one vector to another (subcloning).
Weather. Melbourne enjoys warm summers, glorious springs, mild autumns and crisp winters. With its variable climate, Melbourne hots up December to February (summer), cools down March to May (autumn), chills out June to August (winter), and warms up again September to November (spring). Melbourne's top temperatures are usually in January and February. Those months are often dry, with hot spells and cooling afternoon breezes off Port Phillip Bay. June and July are the coldest months, and October is the wettest.
Victoria in Autumn. Autumn is a lovely season to visit Victoria. September is still warm and dry, but autumn proper arrives in October, as the foliage changes colour and the air cools. With average daily highs of 14Â°C in October, falling to 9Â°C in November, itâs good weather for exploring the coast and for sailing too.ictoriaâs summers are warm but not hot. Average daily highs rise from 18Â°C in June to 21Â°C in August, although nights remain on the cool side at around 11Â°C.