Preparation of DNA for traditional cloning methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together. The DNA to be cloned can vary widely, from genomic DNA extracted from a pure bacterial culture or a mixed population, to a previously cloned gene that needs to be moved from one vector to another (subcloning).
Weather. Melbourne enjoys warm summers, glorious springs, mild autumns and crisp winters. With its variable climate, Melbourne hots up December to February (summer), cools down March to May (autumn), chills out June to August (winter), and warms up again September to November (spring). Melbourne's top temperatures are usually in January and February. Those months are often dry, with hot spells and cooling afternoon breezes off Port Phillip Bay. June and July are the coldest months, and October is the wettest.
Daytime spring temperatures average out at between 17 and 20Â°C, but a high of 40Â°C has been recorded in November, and a low of -0.5 in September, so you can see where the city gets its weather reputation!Spring is a very sporting time in Melbourne, with a particular emphasis on Australian rules football in October.elbourne in the Winter. Melbourne is one of the few Australian state capitals which ever gets cold in the sense that most of us would recognise the word. June, July and August see average daytime temperatures of around 13Â°C, but nights can be positively chilly, with dips below freezing not unknown.