I recently received the following question on cat health from a visitor to my website: My cat has a slight watery discharge from her right eye and it sometimes âcatchesâ dirt as it slides down her nose. I was just wondering if she has an upper respiratory infection and if so, what can I do â¦
The endospore stain is a differential stain used to visualize bacterial endospores. Endospores are formed by a few genera of bacteria, such as Bacillus.By forming spores, bacteria can survive in hostile conditions. Spores are resistant to heat, dessication, chemicals, and radiation.he endospore stain is a differential stain used to visualize bacterial endospores. Endospores are formed by a few genera of bacteria, such as Bacillus.
A differential staining technique using malachite green and safranin . The Schaeffer-Fulton method for staining endospores uses two stains - malachite green and safranin - with heat as the mordant for the malachite green to enter the endospore. Endospores are usually resistant to staining so heat is used to force the stain in. Once the malachite green has entered the endospore, water is used to wash the excess stain away which leaves the rest of the cell easily since the cell wall has been weakened by the heat. The resistance of the endospore prevents the malachite green from exiting.