To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
Temperature dependency: Thermophiles are prevalent in nature and certain prokaryotic species thrive at temperatures above 45 Â°C. The temperature-dependency of Taq makes it optimum at 80 Â°C, where its catalytic activity is more than ten times that typically observed at 37 Â°C.It is possible that the decrease in activity above 80 Â°C is actually due to the denaturation of double-stranded DNA at these temperatures.aq requires the presence of all four species of deoxyribonucleoside triphosphates and DNA for optimum catalytic activity. pH dependency: The pH optimum for the enzyme is within the range of 7-8 pH units when at 80 Â°C, and will vary depending on the buffer system used.