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THE CAPSULE STAIN. The capsule is a thick polysaccharide layer around the outside of the cell. It is nonionic, so. the dyes that we commonly use will not bind to it. Two dyes, one acidic and one basic, are. used to stain the background and the cell wall, respectively. The area between the 2 dyes is. the halo of the capsule surrounding the cell.
The stains of the Capsule staining technique. Typically an acidic stain such as Congo red or nigrosin, which stains the background, and a basic stain that colorizes the cell proper are used.This technique begins as a negative stain; cells are spread in a film with an acidic stain and are not heat-fixed.