For phenomenology, the central feature of experience is a structure called intentionality, which correlates all things experienced with the mode of experience to which the experience is referenced. 2001, Lois Bloom, Erin Tinker, Ellin Kofsky Scholnick, The Intentionality Model and Language Acquisition, page 10,
PAM(Protospacer adjacent motif) sequence in a CRISPR is a 2-6 base pair DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. PAM is a component of the invading virus or plasmid, but is not a component of the bacterial CRISPR locus. Cas9 will not successfully bind to or cleave the target DNA sequence if it is not followed by the PAM sequence.
Real-time polymerase chain reaction. A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR.