To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
Since PCR is, basically, replication of DNA in a test-tube, all the usual ingredients needed for DNA replication are required: A template (the DNA that is being copied) Primers (to initiate the synthesis of the new DNA strands) DNA polymerase (to carry out the synthesis) dNTPs (nucleotide precursors). Cycling temperatures: Of the three temperatures used in a cycle of PCR, the denaturing and extension temperatures remain unchanged for the most part, since most DNA is denatured at 94-95 degrees C, and the temperature optimum for the Taq DNA polymerase lies between 72 and 74 degrees C.