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Gram Staining: A. Add crystal violet stain over the fixed culture. Let stand for 10 to 60 seconds; for thinly prepared slides, it is usually acceptable to pour the stain on and off immediately. Pour off the stain and gently rinse the excess stain with a stream of water from a faucet or a plastic water bottle. Note that the objective of this step is to wash off the stain, not the fixed culture. B. Add the iodine solution on the smear, enough to cover the fixed culture. Let stand for 10 to 60 seconds.
A negative Gram stain is often reported as no organism seen.. This may mean that there is no bacterial infection present or that there were not enough bacteria present in the sample to be seen with the stain under a microscope. Positive Gram stain results usually include a description of what was seen on the slide. 1  This typically includes: 2  Whether the bacteria are Gram-positive (purple) or Gram-negative (pink) 3  Shape â round (cocci) or rods (bacilli) 4  Size, relative quantity, and/or arrangement of the bacteria, if relevant.