To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
On mixing at ambient temperature the two strands of DNA spontaneously form a duplex (C ds) (i.e. Î± = 1) (this is true for DNA strands of any reasonable length). Each sample is heated in a UV spectrometer from 20 ÂºC to (say) 80 ÂºC at a rate of one degree per minute and the absorbance is monitored as shown in Figure 2. The melting temperature, T m, is the temperature at the maximum absorbance gradient (d A /d T) and corresponds to 50% association (Î± = Â½).