To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
Best Answer: Melting temp is when DNA strands separate, annealing temp is the temp where complimentary strands recombine (or anneal).The importance is this: In PCR, the temperature is cylced high to low, and a DNA polymerase that is heat-resistant is used so that it is not denatured at higher temps.f you don't reduce it to that lower temperature, the primers can never hydrogen bond with the DNA and cause extension. and this annealing temperature is CRUCIAL for the PCR to work.