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2.5.3 State that interphase is an active period in the life of a cell when many metabolic reactions occur, including protein synthesis, DNA replication and an increase in the number of mitochondria and/or chloroplasts. During interphase the cell grows larger.
During the S phase the cell replicates the chromosomes by taking one chromatid and effectively doubling it (these are later ripped apart during Anaphase in Mitosis). So during the S phase of the Interphase cycle, chromosomes are replicated into two sister chromatids. Normally after this phase there is a check off to make sure nothing went wrong with the replication however should there be any mutations that will create some new DNA form this is where it would be made.