To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
The lowest temperature (45 ÂºC) is the optimal temperature for cell wall degrading enzymes, Î²-glucanases. The proteases works best at 52 ÂºC, the Î²-amylase best at 63 ÂºC and the Î±-amylase at 72Â°C. The last step in the mashing is inactivation of the enzymes at 78 ÂºC (Figure 3).