To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
The standard PCR enzymes are not surprisingly quite accurate. Even Thermus aquaticus (Taq) DNA polymerase, a low fidelity PCR polymerase, only makes a mistake once in approximately 100,000 nucleotide insertions, depending on reaction conditions (4).The basis of polymerase accuracy is an exciting area of investigation.uring PCR, Taq DNA Polymerase will misincorporate nucleotides at a particular rate leading to mismatch formation. This is termed âerror rateâ. Taq, and polymerases in general, will stall at these mismatched bases and are more likely to dissociate before extending as compared to correctly base paired 3Â´ ends.