To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
Therefore it replaced the DNA polymerase from E. coli originally used in PCR [7]. Taq's temperature optimum for activity is 75-80 0 C, with a half-life of 9 minutes at 97.5 0 C, and can replicate a 10 3 base pair strand of DNA in less than 10 seconds at 72 0 C [8].The incorporation of dNTPs into DNA is catalyzed by Taq DNA polymerase I.aq DNA polymerase has among the highest error rates of the thermophilic polymerases at 285 x 10 -6 errors per template nucleotide. Tli polymerase has a proof reading ability that is five-fold better than Taq at 57 x 10 -6 errors per template nucleotide and Pfu polymerase also demonstrates fidelity in this range [10].