To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
The melting temperature (Tm) is defined as the temperature at which half the DNA strands are in the double-helical state and half are in the random-coil state.The CG content of an oligonucleotide is the most important factor that influences the Tm value.he melting temperature (Tm) is defined as the temperature at which half the DNA strands are in the double-helical state and half are in the random-coil state.