To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
The Rapid DNA Ligation Kit enables sticky-end or. blunt-end DNA ligation in only 5 minutes at room. temperature (see Fig.1).The kit includes T4 DNA. Ligase and a specially formulated 5X Rapid. Ligation Buffer optimized for fast and efficient DNA. ligation. The efficiency of the fast ligation is.