To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
This will amplify a 500 bp product at least 105-fold in 25 cycles, taking about 2.3 minutes per cycle. DNA denaturation is the critical step in the GeneAmpÂ® PCR process and is often the focus of attention if PCR experiments fail. The practical range of denaturation temperatures for most samples is 94 Â°C-96 Â°C.