To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
1 For convenience, ligations may be done at room temperature (20-25Â°C). 2  For cohesive (sticky) ends, use 1 Âµl of T4 DNA Ligase in a 20 Âµl reaction for 10 minutes. 3  For blunt ends, use 1 Âµl of T4 DNA Ligase in a 20 Âµl reaction for 2 hours or 1 Âµl high concentration T4 DNA Ligase for 10 minutes.