To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
Use the plastic alignment jig if you need, or simply mark the position of the blockin some way. 5.2 DNA melting curve experiment 1. Place the block on the hotplate (set to 95 â C) for at least 10 min. Measure the RTD thermistorto make sure the temperature is above 80 â C before removing the block2.