See more pictures of alternative fuel vehicles. You've probably heard about fuel cells. In 2003, President Bush announced a program called the Hydrogen Fuel Initiative (HFI) during his State of the Union Address.
Phenol-Chloroform Extraction. Purpose: Phenol-chloroform extractions are done when you need to purify DNA from a solution that also has proteins. The DNA will dissolve in the aqueous layer, and everything else will go into the non-aqueous layer.. Phenol:chloroform. 1  Add one equal volume of phenol:chloroform:IAA (found in 4C in room 263) to DNA solution. 2  The phenol:chloroform solution has two layers â be sure to take the bottom layer!!! 3  Mix gently; spin for 5 minutes at max speed. 4  Remove aqueous layer (top layer) to a new tube.
Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.