To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
1 If the temperature is too low, the primer could bind imperfectly. 2  If it is too high, the primer might not bind. 3  Typically the annealing temperature is about 3â5 Â°C below the Tm of the primers used. 4  Stable DNAâDNA hydrogen bonds are only formed when the primer sequence very closely matches the template sequence.