See more pictures of alternative fuel vehicles. You've probably heard about fuel cells. In 2003, President Bush announced a program called the Hydrogen Fuel Initiative (HFI) during his State of the Union Address.
Phenol-Chloroform Extraction. Purpose: Phenol-chloroform extractions are done when you need to purify DNA from a solution that also has proteins. The DNA will dissolve in the aqueous layer, and everything else will go into the non-aqueous layer.. Phenol:chloroform. 1  Add one equal volume of phenol:chloroform:IAA (found in 4C in room 263) to DNA solution. 2  The phenol:chloroform solution has two layers â be sure to take the bottom layer!!! 3  Mix gently; spin for 5 minutes at max speed. 4  Remove aqueous layer (top layer) to a new tube.
Chloroform â This is normally used after phenol or phenol/chloroform extractions. While pure chloroform doesnât work as well as the organic solutions mentioned above for protein extraction, it works well for extracting phenol from aqueous solutions.henol versus Phenol/Chloroform versus Chloroform. One of the most frequent questions Iâm asked when training someone is what the differences are between the different organic phases used in extraction. Here is the breakdown, as best as I understand them.