To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
The detection limit of a radioactive probe with a specific activity of 10 8 to 10 9 dpm/Âµg is about 0.5 pg DNA. Thus, for human genomic DNA, 10mg â equivalent to 1.5 pg of a single-copy gene 500 bp in length â is a reasonable minimum quantity to load.