To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
Choosing the Right DNA Polymerase for PCR. Successful PCR depends on two crucial components, an optimized reaction buffer, and a high-quality, thermostable DNA polymerase (such as Taq DNA polymerase). 1. Thermal stability. A denaturation step at approximately 95Â°C in each PCR cycle separates the two strands of a DNA molecule.