For phenomenology, the central feature of experience is a structure called intentionality, which correlates all things experienced with the mode of experience to which the experience is referenced. 2001, Lois Bloom, Erin Tinker, Ellin Kofsky Scholnick, The Intentionality Model and Language Acquisition, page 10,
PAM(Protospacer adjacent motif) sequence in a CRISPR is a 2-6 base pair DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. PAM is a component of the invading virus or plasmid, but is not a component of the bacterial CRISPR locus. Cas9 will not successfully bind to or cleave the target DNA sequence if it is not followed by the PAM sequence.
A: CRISPR âspacerâ sequences are transcribed into short RNA sequences (âCRISPR RNAsâ or âcrRNAâ) capable of guiding the system to matching sequences of DNA. When the target DNA is found, Cas9 â one of the enzymes produced by the CRISPR system â binds to the DNA and cuts it, shutting the targeted gene off. Using modified versions of Cas9, researchers can activate gene expression instead of cutting the DNA. These techniques allow researchers to study the geneâs function.