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The catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme, catalase, is produced by bacteria that respire using oxygen, and protects them from the toxic by-products of oxygen metabolism.he catalase test is used to differentiate staphylococci (catalase-positive) from streptococci (catalase-negative). The enzyme, catalase, is produced by bacteria that respire using oxygen, and protects them from the toxic by-products of oxygen metabolism.
Growth from an overnight culture of the microbe is smeared on a microscope slide. A drop of 3% hydrogen peroxide is added. If copious bubbles are observed, the microbe is positive for catalase.atalase is then added and the reaction is observed. To view the catalase test in VirtualUnknown â¢ Microbiology, complete the following steps: 2. Press the Play button to watch the video. A short video clip showing microscopic observation of a catalase test for the unknown organism will play.