To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
The standard here is Taq polymerase, which has a processivity of 50 -60 nucleotides (nt) per second at 72 o C. The rule of thumb is to simply assume a low-end default processivity for Taq polymerase of 1000 nt per minute bearing in mind that setting your extension times for this assumed value is more than adequate.