See more pictures of alternative fuel vehicles. You've probably heard about fuel cells. In 2003, President Bush announced a program called the Hydrogen Fuel Initiative (HFI) during his State of the Union Address.
Phenol-Chloroform Extraction. Purpose: Phenol-chloroform extractions are done when you need to purify DNA from a solution that also has proteins. The DNA will dissolve in the aqueous layer, and everything else will go into the non-aqueous layer.. Phenol:chloroform. 1  Add one equal volume of phenol:chloroform:IAA (found in 4C in room 263) to DNA solution. 2  The phenol:chloroform solution has two layers â be sure to take the bottom layer!!! 3  Mix gently; spin for 5 minutes at max speed. 4  Remove aqueous layer (top layer) to a new tube.
Simply put, DNA Extraction is the removal of deoxyribonucleic acid (DNA) from the cells or viruses in which it normally resides. DNA is the precipitated by mixing with cold ethanol or isopropanol and then centrifuging. 2  The DNA is insoluble in the alcohol and will come out of solution, and the alcohol serves as a wash to remove the salt previously added.