See more pictures of alternative fuel vehicles. You've probably heard about fuel cells. In 2003, President Bush announced a program called the Hydrogen Fuel Initiative (HFI) during his State of the Union Address.
Phenol-Chloroform Extraction. Purpose: Phenol-chloroform extractions are done when you need to purify DNA from a solution that also has proteins. The DNA will dissolve in the aqueous layer, and everything else will go into the non-aqueous layer.. Phenol:chloroform. 1  Add one equal volume of phenol:chloroform:IAA (found in 4C in room 263) to DNA solution. 2  The phenol:chloroform solution has two layers â be sure to take the bottom layer!!! 3  Mix gently; spin for 5 minutes at max speed. 4  Remove aqueous layer (top layer) to a new tube.
In organic extraction, phenol and chloroform are used to create on organic phase in which cells are lysed and DNA is freed. The DNA remains in the aqueous phase. Ethyl alcohol is used to precipitate the DNA.In the inroganic methos, NaCl and EDTA are used for cell lysis. Following this, an approach similar to the organic method is followed. In solid state extraction, DNA is first precipitated in the presence of high slat and low pH conditions.The precipitated DNA is then adsorbed on to a filter membrane surface.he DNA remains in the aqueous phase. Ethyl alcohol is used to precipitate the DNA. In the inroganic methos, NaCl and EDTA are used for cell lysis. Following this, an approach similar to the organic method is followed.