See more pictures of alternative fuel vehicles. You've probably heard about fuel cells. In 2003, President Bush announced a program called the Hydrogen Fuel Initiative (HFI) during his State of the Union Address.
Phenol-Chloroform Extraction. Purpose: Phenol-chloroform extractions are done when you need to purify DNA from a solution that also has proteins. The DNA will dissolve in the aqueous layer, and everything else will go into the non-aqueous layer.. Phenol:chloroform. 1  Add one equal volume of phenol:chloroform:IAA (found in 4C in room 263) to DNA solution. 2  The phenol:chloroform solution has two layers â be sure to take the bottom layer!!! 3  Mix gently; spin for 5 minutes at max speed. 4  Remove aqueous layer (top layer) to a new tube.
1 DNA is insoluble in ethanol (ethyl alcohol). 2  As ethanol is added to a solution containing DNA, the DNA will come out of solution and stick to whatever is around. 3  At room temperature DNA begins to denature by the action of DNase (present in cell extracts). 4  DNA extraction procedures must be carried on in ice.rocedure: 1  Place 100 ml of homogenizing solution in a beaker heat the solution in a water bath until it reaches 60 C. 2  Mince the onion and add to the solution when it has reached 60 C. Stir and let sit for 15 minutes.