To research the causes of Erdheim-Chester Disease, consider researching the causes of these these diseases that may be similar, or associated with Erdheim-Chester Disease: 1  Polyostotic sclerosing histiocytosis. 2  Bone pain. 3  Retroperitoneal fibrosis. 4  Diabetes insipidus. 5  Exophthalmos. 6  Dyspnea. 7  Ataxia.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.
As shown in Figure 13, the reaction rate increases with temperature to a maximum level, then abruptly declines with further increase of temperature. Because most animal enzymes rapidly become denatured at temperatures above 40Â°C, most enzyme determinations are carried out somewhat below that temperature. Over a period of time, enzymes will be deactivated at even moderate temperatures. Storage of enzymes at 5Â°C or below is generally the most suitable.