DWS has built work in Colorado, Texas, British Columbia, Utah and New Mexico.
Gram Staining: A. Add crystal violet stain over the fixed culture. Let stand for 10 to 60 seconds; for thinly prepared slides, it is usually acceptable to pour the stain on and off immediately. Pour off the stain and gently rinse the excess stain with a stream of water from a faucet or a plastic water bottle. Note that the objective of this step is to wash off the stain, not the fixed culture. B. Add the iodine solution on the smear, enough to cover the fixed culture. Let stand for 10 to 60 seconds.
A gram stain that does not show the presence of bacteria does not rule out a wound infection. A wound culture may also sometimes be ordered on an individual who has undergone treatment for a wound infection, to determine whether the treatment was effective. It may also be ordered at intervals on a person who has a chronic infection, to help guide further treatment.