See more pictures of alternative fuel vehicles. You've probably heard about fuel cells. In 2003, President Bush announced a program called the Hydrogen Fuel Initiative (HFI) during his State of the Union Address.
Phenol-Chloroform Extraction. Purpose: Phenol-chloroform extractions are done when you need to purify DNA from a solution that also has proteins. The DNA will dissolve in the aqueous layer, and everything else will go into the non-aqueous layer.. Phenol:chloroform. 1  Add one equal volume of phenol:chloroform:IAA (found in 4C in room 263) to DNA solution. 2  The phenol:chloroform solution has two layers â be sure to take the bottom layer!!! 3  Mix gently; spin for 5 minutes at max speed. 4  Remove aqueous layer (top layer) to a new tube.
Phenol/Chloroform â This is a mixture of buffer-saturated phenol and chloroform, usually close to 1:1 for DNA purification with other ratios sometimes used for RNA purification. Isoamyl alcohol is sometimes included as an anti-foaming agent, but is generally thought to be an inert and optional addition.hloroform â This is normally used after phenol or phenol/chloroform extractions. While pure chloroform doesnât work as well as the organic solutions mentioned above for protein extraction, it works well for extracting phenol from aqueous solutions.