It is for this reason that is referred to as a complementary DNA or cDNA. Though there are numerous processes for synthesizing cDNA, the best way of doing so is by using mature or fully spliced mRNA. This is usually done by using the enzyme reverse transcriptase.
Ammonia is used as refrigerant prominently in the refrigeration systems of food industry like dairies, ice creams plants, frozen food production plants, cold storage warehouses, processors of fish, poultry and meat and number of other applications.
1 A domestic refrigerator is present in 99.5% of American homes. 2  The earliest units used toxic refrigerants, typically ammonia (R-717), sulfur dioxide (R-764), or methyl chloride (R-40) as their refrigerant. 3  In the 1930s R-12 was used to replace sulphur dioxide as the most commonly used refrigerant. A domestic refrigerator is present in 99.5% of American homes. 2  The earliest units used toxic refrigerants, typically ammonia (R-717), sulfur dioxide (R-764), or methyl chloride (R-40) as their refrigerant. 3  In the 1930s R-12 was used to replace sulphur dioxide as the most commonly used refrigerant.