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Bacterial neutral proteases are active in a narrow pH range (pH 5 to 8) and have relatively low thermotolerance. Due to their intermediate rate of reaction, neutral proteases generate less bitterness in hydrolyzed food proteins than do the animal proteinases and hence are valuable for use in the food industry. Neutrase, a neutral protease, is insensitive to the natural plant proteinase inhibitors and is therefore useful in the brewing industry. The bacterial neutral proteases are characterized by their high affinity for hydrophobic amino acid pairs.
Principle And Interpretation. Brewer (1) devised this medium for use with Brewer anaerobic cover to permit surface growth of anaerobes and microaerophiles. on agar without the use of anaerobic jar. This medium is suitable for isolation of facultative and obligate anaerobes and for the. study of colonial morphology as colonies can be readily seen on the light coloured agar and are easily accessible (2,3). Dispense 50-60 ml medium per 95 x 20 mm plate.