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Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates.oagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. The fibrin clot may protect the bacterium from phagocytosis and isolate it from other defenses of the host.
The coagulase tube test has been set up as a demo for you to observe in class. This version of the coagulase test is used to identify the presence of either bound coagulase or free coagulase, which is an extracellular enzyme.Free coagulase reacts with a component of plasma called coagulase-reacting factor.The result is to cause the plasma to coagulate. In the demo, the coagulase plasma has been inoculated with Staphylococcus aureus and Staphylococcus epidermidis and allowed to incubate at 37ËC for 24 hours.ound coagulase reacts with the fibrinogen in plasma, causing the fibrinogen to precipitate. This causes the cells to agglutinate, or clump together, which creates the âlumpyâ look of a positive coagulase slide test.