To prepare 1 L of either 1Ã or 10Ã PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
Cardiovascular disease is a term that refers to more than one disease of the circulatory system including the heart and blood vessels, whether the blood vessels are affecting the lungs, the brain, kidneys or other parts of the body. Cardiovascular diseases are the leading cause of death in adult Canadian men and women.
Because the respiratory system is made up of so many different tissues and organs, there are a lot of things that can go wrong and subsequently a lot of different diseases that can affect it.Some of the many diseases that can affect the respiratory system include: 1  Laryngitis-Swelling and irritation of the larynx.2  Bronchitis-An infection in your bronchial tubes. 3  Tonsillitis-Inflammation of the tonsils. 4  Pneumonia-An infection deep in your lungs.ecause the respiratory system is made up of so many different tissues and organs, there are a lot of things that can go wrong and subsequently a lot of different diseases that can affect it.