To prepare 1 L of either 1Ã or 10Ã PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
Cardiovascular disease is a term that refers to more than one disease of the circulatory system including the heart and blood vessels, whether the blood vessels are affecting the lungs, the brain, kidneys or other parts of the body. Cardiovascular diseases are the leading cause of death in adult Canadian men and women.
A: Diseases that affect the human body's muscular system include genetic disorders such as muscle dystrophy and cancers such as soft tissue sarcoma, according to MedlinePlus. Glycogen-storage diseases, tetany and myotonia congenita can also affect the muscular system, notes Encyclopaedia EncyclopÃ¦dia. Britannica Know. more