To prepare 1 L of either 1Ã or 10Ã PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
Cardiovascular disease is a term that refers to more than one disease of the circulatory system including the heart and blood vessels, whether the blood vessels are affecting the lungs, the brain, kidneys or other parts of the body. Cardiovascular diseases are the leading cause of death in adult Canadian men and women.
The circulatory system is the bodyâs blood transport system. A circulatory disorder is any disorder or condition that affects the circulatory system. Circulatory disorders can arise from problems with the heart, blood vessels or the blood itself.Disorders of the circulatory system generally result in diminished flow of blood and oxygen supply to the tissues.irculatory disorders can arise from problems with the heart, blood vessels or the blood itself. Disorders of the circulatory system generally result in diminished flow of blood and oxygen supply to the tissues.