To prepare 1 L of either 1Ã or 10Ã PBS, dissolve the reagents listed above in 800 mL of H2O. Adjust the pH to 7.4 (or 7.2, if required) with HCl, and then add H2O to 1 L. Dispense the solution into aliquots and sterilize them by autoclaving for 20 min at 15 psi (1.05 kg/cm2) on liquid cycle or by filter sterilization. Store PBS at room temperature.
Cardiovascular disease is a term that refers to more than one disease of the circulatory system including the heart and blood vessels, whether the blood vessels are affecting the lungs, the brain, kidneys or other parts of the body. Cardiovascular diseases are the leading cause of death in adult Canadian men and women.
Heart disease is the general term for all diseases and disorders that affect the heart and blood vessels, such as heart attack, stroke, coronary artery disease, and congenital or present at birth heart defects, as well as arrhythmia, angina and cardiomyopathy.Heart disease affects the body in several ways.As mentioned above, the circulatory system, which is also called cardiovascular system, has the role to supply the body cells and tissues with oxygen and nutrients, and therefore, the healthy circulatory system is vital for the survival.eart disease is the general term for all diseases and disorders that affect the heart and blood vessels, such as heart attack, stroke, coronary artery disease, and congenital or present at birth heart defects, as well as arrhythmia, angina and cardiomyopathy. Heart disease affects the body in several ways.