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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
What is the function of the group of amino acids on the RNA polymerase, called the rudder? There are 3 principles to keep in mind when predicting the sequence of mRNA produced by transcription of a particular DNA sequence: 1. RNA polymerase reads only from the template DNA strand. 2. RNA polymerase reads the code form the template in a 3' to 5' direction and produces mRNA in a 5' to 3' Direction.