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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
SUMMARY: 1.DNA polymerase synthesizes DNA while RNA polymerase synthesizes RNA. 2.In contrast with the DNA polymerase, RNA polymerases do not necessarily require the so called primer to start the process and they actually have no proofreading systems.2.RNA polymerases are capable of initiating a new strand but DNA polymerases cannot. 3.DNA has the base thymine while RNA has uracil as its base.UMMARY: 1.DNA polymerase synthesizes DNA while RNA polymerase synthesizes RNA. 2.In contrast with the DNA polymerase, RNA polymerases do not necessarily require the so called primer to start the process and they actually have no proofreading systems.