BONUS: your company's applications - all this infrastructure is here to support your company's applications. Understand what they do, why they are important, which ones make money, and how they work together. Do this by talking to application owners and using the applications yourself.
Trehalose, also known as mycose or tremalose, is a natural alpha-linked disaccharide formed by an Î±,Î±-1,1-glucoside bond between two Î±- glucose units.he two prevalent theories as to how trehalose works within the organism in the state of cryptobiosis are the vitrification theory, a state that prevents ice formation, or the water displacement theory, whereby water is replaced by trehalose, although it is possible that a combination of the two mechanisms is at work.
These effects of trehalose on several enzymes commonly used in molecular biology are summarized in Table 1. Several enzymes, such as MMLV RT, Sty I, Eco RI, Nco I, DNaseI, RNaseI, Nde I, Pvu II, and Pst I, were thermostabilized, and a subset of them also could be thermoactivated.igure 2. Improvement of full length cDNA yield by thermostabilization of RT by trehalose. (A) 5-kb cDNAs were synthesized in the presence of 0.6 M trehalose at 45Â°C (lane 1), 55Â°C (lane 2), 60Â°C (lane 3), and 65Â°C (lane 5); lane 4, synthesis at 60Â°C in the absence of trehalose.