With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
1. Separating the Target DNA-Denaturation. During the first step of PCR, called denaturation, the tube containing the sample DNA is heated to more than 90 degrees Celsius (194 degrees Fahrenheit), which separates the double-stranded DNA into two separate strands.