With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
Protein absorbs strongly at 280 nm due to a number of its constituent amino acids. The peptide bonds found in the amino acids also absorb at 205 nm. The UV absorbance of protein can be used both to quickly image and acquire spectra of microscopic samples non-destructively. The spectra can also be used to determine protein concentrations and the relative amounts of protein to DNA or RNA.