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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
Answers. Best Answer: RNA polymerase is used in Transcription while Helicase is used in DNA replication. RNA polymerase is an enzyme that will help to unwind the double stranded DNA by breaking the hydrogen bonds between complementary bases. Basically, RNA polymerase is a wonderful enzyme which does all the work from breaking to adding complementary RNA nucleotides.