Clinical observations include estimation of haemoglobin-oxygen saturation (SpO 2, pulse oximetry), oxygen delivery, respiratory rate, respiratory distress, heart/pulse rate, blood pressure (systolic, diastolic and mean), temperature, level of consciousness OR level of sedation, and a pain score.linical observations include estimation of haemoglobin-oxygen saturation (SpO 2, pulse oximetry), oxygen delivery, respiratory rate, respiratory distress, heart/pulse rate, blood pressure (systolic, diastolic and mean), temperature, level of consciousness OR level of sedation, and a pain score.
In turn this triggers the sarcoplasmic reticulum to release calcium ions into the muscle interior where they bind to troponin, thus causing tropomyosin to shift from the face of the actin filament to which myosin heads need to bind to produce contraction.
During skeletal muscle contraction calcium ions expose myosin  binding sites on to the actin. When a nerve sends a signal to a  muscle to do some action the release of ace â¦ tylcholine at the  neuromuscular junction triggers calcium to be released into the  cytoplasm.