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SIM test is a test performed to test for sulfur reduction, Indole production and motility. Black color formed= positive for sulfur reduction Red color after adding Kovacs reag â¦ ent= positive for Indole production Microorgaism growth all over the tube= motile.
Staphylococcus aureus is negative for motility, and positive for the indole production. The SIM test tests for sulfur reduction, indole production and motility. Positive for i â¦ ndole production means tryptophan is broken down into indole and pyruvate and will give a red color.