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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
RNA polymerase. Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription.NA polymerase. Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription.