Clinical observations include estimation of haemoglobin-oxygen saturation (SpO 2, pulse oximetry), oxygen delivery, respiratory rate, respiratory distress, heart/pulse rate, blood pressure (systolic, diastolic and mean), temperature, level of consciousness OR level of sedation, and a pain score.linical observations include estimation of haemoglobin-oxygen saturation (SpO 2, pulse oximetry), oxygen delivery, respiratory rate, respiratory distress, heart/pulse rate, blood pressure (systolic, diastolic and mean), temperature, level of consciousness OR level of sedation, and a pain score.
In turn this triggers the sarcoplasmic reticulum to release calcium ions into the muscle interior where they bind to troponin, thus causing tropomyosin to shift from the face of the actin filament to which myosin heads need to bind to produce contraction.
A quick review of excitation-contraction coupling in skeletal muscle: The sarcoplasmic concentration of calcium in skeletal muscle is kept very low by the action of Ca-ATPases which remove the calcium and sequester it in the sarcoplasmic reticulum. When an action potential depolarizes the muscle cell membrane, it also travels into the body of the cell via the t-tubules.