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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
see pcr1. RNA polymerase. 1. an enzyme that synthesizes an RNA copy of the sequence in a limited region of DNA in a process known as DNA transcription. Called also DNA-dependent RNA polymerase. 2. a viral enzyme that synthesizes RNA from an RNA template during viral replication. Called also RNA-dependent RNA polymerase. see RNA polymerase (above). Taq polymerase. a DNA polymerase that functions at high temperature; derived from the bacterium Thermus aquaticus and used in the polymerase chain reaction.