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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
DNA polymerase 3 is also called as holoenzyme and it is the most essential component of replisome. Difference in function. DNA polymerase 1 functions helps in DNA replication. It is used for molecular biology research. During the process of replication, an RNA primer is filled in the lagging strand of the DNA.DNA polymerase 1 removes the RNA primer and fills in the nucleotides which are necessary for the formation of the DNA in the direction- 5â to 3â.t is used for molecular biology research. During the process of replication, an RNA primer is filled in the lagging strand of the DNA. DNA polymerase 1 removes the RNA primer and fills in the nucleotides which are necessary for the formation of the DNA in the direction- 5â to 3â.