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SIM test is a test performed to test for sulfur reduction, Indole production and motility. Black color formed= positive for sulfur reduction Red color after adding Kovacs reag â¦ ent= positive for Indole production Microorgaism growth all over the tube= motile.
The indole test is a component of the IMViC series of tests, which is used for differentiating the Enterobacteriaceae. The motility test is useful for testing a wide variety of organisms. As a whole, the SIM test is primarily useful for differentiating Salmonella and Shigella.