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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
DNA polymerase adds nucleotides onto the 3'OH end of the preceding nucleotide and carries out synthesis in the 5' to 3' direction down the length of the DNA. The result of DNA polymerase function is two copies of DNA that contain one original and one newly synthesized strand of DNA.To unlock this lesson you must be a Study.com Member.he result of DNA polymerase function is two copies of DNA that contain one original and one newly synthesized strand of DNA. To unlock this lesson you must be a Study.com Member.