With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
DNA is quite soluble in water and invisible, while it is insoluble in alcohol wherein it precipitates and becomes visible. By adding alcohol to the DNA filtrate solution in the tube, the DNA is rendered visible. Figure 10 - Very slowly, pour some ice cold alcohol into.