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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
RNA polymerase uses RNA as a template and DNA polymerase uses DNA as a template b. RNA polymerase does not need to separate the two strands of DNA in order to synthesize RNA, whereas DNA polymerase must separate the DNA strands before it can replicate DNA c.NA polymerase does not need to separate the two strands of DNA in order to synthesize RNA, whereas DNA polymerase must separate the DNA strands before it can replicate...