With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
Concentration and Purity by. Ultraviolet Spectrophotometry. Typically, the first thing one wants to know about a DNA preparation is its concentration and purity. Both. can be determined by measuring the absorption of ultraviolet light. DNA absorbs UV more or less strongly. depending upon the wavelength. We will be using a NanoDrop spectrophotometer, which takes.