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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
Basically, RNA polymerase's role is very similar to that of DNA polymerase. RNA polymerase is an enzyme that is used during transcription in the nucleus. Similar to DNA polyme â¦ rase, RNA polymerase codes for the complementary nucleotides to a DNA strand.NA polymerase creates a strand of DNA from a template strand of DNA. it is used to create a new copy of the DNA. In order to begin DNA synthesis, a primer is necessary. The primer is a short oligo nucleotide fragment that hybridizes to the template strand of DNA (the strand which is to be copied).