With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
The melting temperature (Tm) is defined as the temperature at which half the DNA strands are in the double-helical state and half are in the random-coil state.The CG content of an oligonucleotide is the most important factor that influences the Tm value.he melting temperature (Tm) is defined as the temperature at which half the DNA strands are in the double-helical state and half are in the random-coil state.