On Sept.3, 1866, during the Battle of Curuzu, one of the battles of the war of Triple Alliance, the Paraguayan 10th battalion fled without firing a shot. President Lopez ordered the decimation of the battalion, which was accordingly formed in line and each tenth man shot.
phenol is more acidic because of the benzene ring present in the molecule,when you lose the H form the OH group it is possible to delocalise the charge around the aromatic system due to the pi electron cloud,straight chain alcohols cannot do this so it is less favourable to deprotonate them hance it is easier to deprotonate a phenol,hence we say it ...
1 The pH is important since chromosomal DNA will end up in the phenol phase if the pH is acid (arround pH 5). 2  This fact can be used for RNA extraction but it is not a good idea to use acid phenol to clean your chromosomal DNA from proteins.t is typically easiest to carry the extraction out in 1.7â2 mL eppendorf tubes. 1  Dilute your nucleic acid sample to 100â700 ÂµL or divide your samples into tubes such that you have no more than 700 ÂµL per tube. 2  Add an equal volume of phenol to the tube, vortex vigorously to mix the phases. 3  Spin in a microfuge at top speed for 1â2 min to separate ...