With more education and experience, accountants can become certified. Some financial documents, such as those submitted to the Securities and Exchange Commission, must be signed by a Certified Public Accountant.
Hyperchromicity is the increase of absorbance (optical density) of a material. The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level.
DNA purity (A 260 /A 280) = (A 260 reading â A 320 reading) Ã· (A 280 reading â A 320 reading). Strong absorbance around 230nm can indicate that organic compounds or chaotropic salts are present in the purified DNA. A ratio of 260nm to 230nm can help evaluate the level of salt carryover in the purified DNA.otal yield is obtained by multiplying the DNA concentration by the final total purified sample volume. DNA yield (Âµg) = DNA concentration Ã total sample volume (ml). However, DNA is not the only molecule that can absorb UV light at 260nm.