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The second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.he second two activities of DNA Pol I are important for replication, but DNA Polymerase III (Pol III) is the enzyme that performs the 5'-3' polymerase function. 1  Primase-The requirement for a free 3' hydroxyl group is fulfilled by the RNA primers that are synthesized at the initiation sites by these enzymes.
Quick Answer. The function of RNA polymerase II is to synthesize mRNA using a DNA strand as a template, explains UC Davis BioWiki. The process, by which an mRNA strand is constructed from a DNA strand, is called transcription, and the product is called a primary transcript, notes North Dakota State University. Continue Reading.