Acute kidney injury is defined as an abrupt (within 48 hours) reduction in kidney function based on an elevation in serum creatinine level, a reduction in urine output, the need for renal replacement therapy (dialysis), or a combination of these factors.
5. Autoclave using the appropriate cycle/settings (a typical decontamination cycle is 15-60min at 121Â°C and 15psi). A C B. (Created 3/02; Revised 5/05, 5/08, 9/08, 5/10, 8/12) UNL Environmental Health and Safety Â· (402) 472-4925 Â· http://ehs.unl.edu 7 6.
4. Run the autoclave. The autoclave machine should run for a certain period of time at a specific temperature and pressure. Wrapped instruments should be in the autoclave at 250 degrees for 30 minutes at 15 PSI or 273 degrees for 15 minutes at 30 PSI.Once the machines has run, you need to open the door a small bit to let the steam out. Make sure that any instrument that can open, such as scissors, is left open when you put it into a pouch. 2  The inside of the instruments need to be sterilized as well. 3  Autoclaving in pouches is convenient because you can see the instruments you need when finished.