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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
During cell division, the identical copies (called a sister chromatid pair) are joined at the region called the centromere (2). Once the paired sister chromatids have separated from one another (in the anaphase of mitosis) each is known as a daughter chromosome.