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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
The cells that enter meiosis II are the ones made in meiosis I. These cells are haploidâhave just one chromosome from each homologue pairâbut their chromosomes still consist of two sister chromatids. In meiosis II, the sister chromatids separate, making haploid cells with non-duplicated chromosomes. Phases of meiosis II Prophase II: Starting cells are the haploid cells made in meiosis I. Chromosomes condense. Metaphase II: Chromosomes line up at the metaphase plate. Anaphase II: Sister chromatids separate to opposite ends of the cell. Telophase II: Newly forming gametes are haploid, and each chromosome now has just one chromatid.