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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Chromosome. Image courtesy of Indigo Instruments. Definition: Sister chromatids are two identical copies of a single chromosome that are connected by a centromere. Examples: In anaphase of mitosis, sister chromatids separate and begin moving to opposite ends of the cell. Once the paired sister chromatids separate from one another, each is considered a full chromosome.