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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
The two chromatids of each sister chromatid pair are segregated into separate cells in both mitosis and meiosis. But they remain together throughout the first division during meiosis (meiosis I).It is only during the second meiotic division (during anaphase II) that they finally separate. single replicated chromosome has two sister chromatids. One chromatid is shown in yellow, the other in blue. The two chromatids contain exactly identical chains of double-stranded DNA, as well as a protein support structure composed of mostly of histones. Histones are proteins present in all eukaryotic cells.