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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Sister chromatids remain attached until anaphase of mitosis or anaphase II of meiosis. Sister chromatid separation ensures that each daughter cell gets the appropriate number of chromosomes after division. In humans, each mitotic daughter cell would be a diploid cell containing 46 chromosomes.