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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Sister chromatids (pronounced KRÅ-mÉ-tidz) are the two identical halves of a single replicated eukaryotic chromosome. They are joined at the centromere (see diagram at right) and genetically identical because, during interphase, they are produced from the two complementary strands of a single duplex DNA molecule (). single replicated chromosome has two sister chromatids. One chromatid is shown in yellow, the other in blue. The two chromatids contain exactly identical chains of double-stranded DNA, as well as a protein support structure composed of mostly of histones. Histones are proteins present in all eukaryotic cells.