Typical costs: 1  Costs for a groom's cake are similar to those for wedding cakes, ranging from $1-$2 or less per slice for a relatively straightforward single-tier or sheet cake, to $8-$10 or more a slice for elaborate cakes with fondant, gum paste and sugar decorations or fruit.
Immunofluorescence microscopy is used to localize specific constituents in tissue sections or immobilized cells using fluorescent tags as labels.
Immunofluoresence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. Several microscope designs can be used for analysis of immunofluorescence samples; the simplest is the epifluorescence microscope, and the confocal microscope is also widely used.Various super-resolution microscope designs that are capable of much higher resolution can also be used.econdary, or indirect, immunofluorescence uses two antibodies; the unlabeled first (primary) antibody specifically binds the target molecule, and the secondary antibody, which carries the fluorophore, recognises the primary antibody and binds to it. Multiple secondary antibodies can bind a single primary antibody.