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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
The two chromatids of each sister chromatid pair are segregated into separate cells in both mitosis and meiosis. But they remain together throughout the first division during meiosis (meiosis I).It is only during the second meiotic division (during anaphase II) that they finally separate.ister chromatids (pronounced KRÅ-mÉ-tidz) are the two identical halves of a single replicated eukaryotic chromosome. They are joined at the centromere (see diagram at right) and genetically identical because, during interphase, they are produced from the two complementary strands of a single duplex DNA molecule ().