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There are two main strategies for the induction of transcription: a strong promoter is negatively controlled by a repressor protein and induction of transcription is effected by an inducer (a ligand that inactivates the repressor).
An inducer (allolactose or an analog) binds to the repressor and prevents its binding to the operator, thereby releasing the repression and allowing transcription of the lac operon. a) Most mutations in the operator, the binding site for repressor, lead to lower affinity for the repressor and hence less binding.n up mutation would make the lac operon no longer dependent on the positive regulation by the cAMPâCAP complex (when the operon is induced). A down mutation would not allow expression even in the deârepressed state (presence of inducer) and hence would show a nonâinducible phenotype. 15.3 a) Choice (c) is correct.