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The infusion time should not exceed 4 hours. Platelets, plasma and cryoprecipitate generally are transfused at a rate of 10 ml per minute. In order for a patient to receive maximum benefit platelets need to be transfused rapidly to get control of bleeding.
Procedures for Transfecting Adherent Cells: Cell Seeding (see Table 1): Cells should be plated 18 to 24 hours prior to transfection so that the. monolayer cell density reaches to the optimal ~70% confluency at the. time of transfection. Complete culture medium with serum and.