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We amplified a 466-bp fragment of KRAS encoding exon 2 and partial intron 2, as well as a 1564-bp fragment of p53 spanning from exon 5 to exon 8, including introns 5, 6, and 7, from both cell lines and DNA isolated from exosomes derived from the same cell lines (Fig. 2A).
Isolation of plasmid DNA and analysis of isolated plasmid. Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words, this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells.You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a).To this end you have (1) isolated total chromosomal DNA from your assigned bacterial strain and digested this DNA with the restriction enzyme BamHI.o this end you will digest 10 uL of the isolated plasmid with restriction enzyme BamHI. The 40 uL of the plasmid DNA will be quantified using Nanodrop spectro-photometry. A predetermined amount of the DNA will be submitted for sequence analysis.