Ciliate conjugation is a sexual phenomenon that results in genetic recombination and nuclear reorganization within the cell. In most ciliate groups, however, the cells separate after conjugation, and both form new macronuclei from their micronuclei.
Before the bun, hamburgers are said to have been served between two pieces of bread. In fact, a ground beef patty was known as Hamburger steak (first mentioned in an American cookbook in 1891); when this was put between bread or in a bun it was called a Hamburger sandwich.
Charlie Hamburger Nagreen made claim to having served the first hamburger sandwich in 1885 at the Outgamie County Fair in Seymour, Wisc. He was 15 at the time. His concoction is variously described as a flattened meatball and butter-fried ground beef. In either event, he stuck it between slices of bread.