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We amplified a 466-bp fragment of KRAS encoding exon 2 and partial intron 2, as well as a 1564-bp fragment of p53 spanning from exon 5 to exon 8, including introns 5, 6, and 7, from both cell lines and DNA isolated from exosomes derived from the same cell lines (Fig. 2A).
When she is ready to extract DNA, she cuts a small piece of bone or tooth and crushes it into powder. It looks a little like flour you can bake with. In the bone powder are millions of bone cells and millions of cells from other organisms, including bacteria and parasites. A copy of the DNA is located inside every single cell. Next, she needs to crack open the cell walls so that the DNA spills out. A special enzyme does this job.