In August 2015, Morris self-released five songs as a self-titled extended play, Maren Morris, on Spotify. The songs garnered 2.5 million streams on Spotify in a month, with three songs appearing in Spotify's US and Global Viral 50 chart.
Differential Staining. Gram stain technique is a differential staining technique, which separates bacteria into two groups (discussed in earlier chapters), Gram-positive bacteria and Gram-negative bacteria. Another differential staining technique is acid-fast technique which differentiates species of Mycobacterium from other bacteria.
A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope. Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining technique and the eosin counterstain to haematoxylin in the H&E stain. In Gram staining, crystal violet stains only Gram-positive bacteria, and safranin counterstain is applied which stains all cells, allowing the identification of Gram-negative bacteria as well. An alternative method uses dilute carbofluozide.