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We amplified a 466-bp fragment of KRAS encoding exon 2 and partial intron 2, as well as a 1564-bp fragment of p53 spanning from exon 5 to exon 8, including introns 5, 6, and 7, from both cell lines and DNA isolated from exosomes derived from the same cell lines (Fig. 2A).
Describe how a sample of DNA is collected and prepared for study. First the cells must be collected. If the cells are part of a hard surface or sample, they must be washed from the sample into a liquid solvent. If they are part of a soft tissue, then they are easier to isolate and remove. [ Once the cellular material is isolated, the cell membrane and associated proteins must be broken down to allow access to the DNA buried in the center of the cell. This. is done with surfactants, or soaps.