Use the chart below for amounts. Paste up your dye with the urea water (see below), then add rest of water and stir 'till thoroughly dissolved. Pour into squeeze bottles with a funnel. An already wet, tied up adult tee will absorb about 4 oz of liquid dye, depending on how much you apply. Use this as a guide to help you decide how much to mix up.
Bdellovibrio bacteriovorus is a small, motile, deltaproteobacterium that is a predatory invader of other gram-negative bacteria. It occupies an intraperiplasmic niche and kills, digests, and lyses its host, the prey cell. The predatory (host-dependent, or HD) process is summarized in Figure 1.
The sequenced genome of HD100 was surprisingly large and suggests that Bdellovibrio predatory life is far from obligately parasitic, with seemingly none of the reductive evolution that a parasitic lifestyle has for bacteria such as Mycobacterium leprae and Yersinia pestis.