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In the male, the principal sex hormones are the androgens, of which testosterone is the most important. The androgens are steroid hormones produced mainly by the Leydig cells(interstitial cells located near the seminiferous tubules) of the Testes.
The fluid in the lumen of seminiferous tubules is quite different from plasma; it contains very little protein and glucose but is rich in androgens, estrogens, potassium, inositol and Glutamic and Aspartic acid. This composition is maintained by Blood-testis barrier.hen the bloodâtestes barrier is breached, and sperm enters the bloodstream, the immune system mounts an autoimmune response against the sperm. The anti-sperm antibodies generated by the immune system can bind to various antigenic sites on the surface of the sperm.