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Quantitation of changes in cell shape using computerized image analysis demonstrated that PDGF doubled the total projected area of lamellipodia formed by each cell within 5 min of addition and increased it by almost 10-fold at 30 min (Fig. 3 â A).o determine relative changes in lamellipodia length in different regions of the cell, corners were defined as parts of the square perimeter within 6 Î¼m from the intersection of the two sides; sides were defined as the 18 Î¼m interval between these corner regions.
Annulus Fibrosus and Nucleus Pulposus. Intervertebral discs are composed of an annulus fibrosus and a nucleus pulposus. The annulus fibrosus is a strong radial tireâlike structure made up of lamellae; concentric sheets of collagen fibers connected to the vertebral end plates.The sheets are orientated at various angles.he annulus fibrosus encloses the nucleus pulposus. Although both the annulus fibrosus and nucleus pulposus are composed of water, collagen, and proteoglycans (PGs), the amount of fluid (water and PGs) is greatest in the nucleus pulposus. PG molecules are important because they attract and retain water.