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Quantitation of changes in cell shape using computerized image analysis demonstrated that PDGF doubled the total projected area of lamellipodia formed by each cell within 5 min of addition and increased it by almost 10-fold at 30 min (Fig. 3 â A).o determine relative changes in lamellipodia length in different regions of the cell, corners were defined as parts of the square perimeter within 6 Î¼m from the intersection of the two sides; sides were defined as the 18 Î¼m interval between these corner regions.
Abstract. Lamellipodia formation necessary for cell invasion is regulated by Rac1. We report here that lamellipodia formation and three-dimensional invasion were significantly promoted by HGF and serum, respectively, in invasive human breast cancer cells.urther clarification of the mechanisms by which PI3K activation induces the release of IQGAP1 and kinesin heavy chain from Rac1 and serum promotes three-dimensional cell invasion should lead to an increased understanding of the regulation of lamellipodia formation and cell invasion.