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Haptoglobin, bilirubin, and liver function tests. When red blood cells break down, they release hemoglobin into the bloodstream. The hemoglobin combines with a chemical called haptoglobin. A low level of haptoglobin in the bloodstream is a sign of hemolytic anemia.
Deficiency of iron leads to decreased production of hemoglobin; low levels of hemoglobin in turn leads to production of smaller and hypochromic RBCs. Blood loss; diet low in iron; poor absorption of iron: Pernicious Anemia and B Vitamin Deficiency