Radio Stars. Quasars are the brightest and most distant objects in the known universe. In the early 1960's, quasars were referred to as radio stars because they were discovered to be a strong source of radio waves. In fact, the term quasar comes from the words, quasi-stellar radio source.
The following DNA sequence occurs in the nontemplate strand of a structural gene in bacterium (the promoter sequence is located to the left but is not shown): 5 -GAATGTCAGAACTGCCATGCTTCATATGAATAGACCTCTAG-3 What is the ribonucleotide sequence of mRNA molecule that is transcribed from this piece of DNA?
cDNA construction [edit]. Once mRNA is purified, oligo-dT (a short sequence of deoxy-thymine nucleotides) is tagged as a complementary primer which binds to the poly-A tail providing a free 3'-OH end that can be extended by reverse transcriptase to create the complementary DNA strand.DNA Library vs. Genomic DNA Library [edit]. cDNA library lacks the non-coding and regulatory elements found in genomic DNA. Genomic DNA libraries provide more detailed information about the organism, but are more resource-intensive to generate and maintain.