1 Completion of a coding training program. 2  At least two years of experience in the field. 3  CCA certificate and at least one year of experience in the field.  Coding certificate from another certifying body and at least one year of experience.
Rating Newest Oldest. Best Answer: No, plasmids are not necessary to allow bacteira to survive .Plasmids are typically not strictly for survival and are only beneficial under certain conditions. The DNA necessary for bacterial life is usually in the chromosomal DNA. The plasmid DNA may confer benefits but the function of most plasmid DNA is currently unknown. Scientists have selected for plasmid free bacteria, and plasmid-free strains, especially E. coli, are available commercially.
In their simplest form, plasmids require a bacterial origin of replication, an antibiotic resistance gene, and at least one unique restriction enzyme recognition site. These elements allow for the propagation of the plasmid within bacteria, while allowing for selection against any bacteria not carrying the plasmid. Self-replicating-Once you have constructed a plasmid, you can make an endless number of copies of the plasmid by growing the plasmid in bacteria. 2  Stable-Plasmids are stable long-term either as purified DNA or within bacteria (as glycerol stocks).