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Haptoglobin, bilirubin, and liver function tests. When red blood cells break down, they release hemoglobin into the bloodstream. The hemoglobin combines with a chemical called haptoglobin. A low level of haptoglobin in the bloodstream is a sign of hemolytic anemia.
Hemoglobin is a protein in your red blood cells that carries oxygen. Folate, or folic acid -- also known as vitamin B-9 -- is needed to produce red blood cells, which house homoglobin. Your hemoglobin will become low if you do not get enough folic acid. This is called anemia.