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Procedure. 1. The independent variables in this science fair project are the temperature and species of bacteria-E Coli, Streptococcus or Bacillus. The dependent variable is the size of bacteria growth after five days, which is determined by measuring the size of bacterial growth with a ruler.bservation. Bacteria that were exposed to temperatures of 60 degrees Celsius and 100 degrees Celsius were observed not to have any growth when transferred to the Petri dishes, whereas bacteria exposed to -78 degrees Celsius and 0 degrees Celsius were observed to have a significant amount of growth.