Triggers of nonallergic rhinitis symptoms vary and can include certain odors or irritants in the air, changes in the weather, some medications, certain foods, and chronic health conditions. A diagnosis of nonallergic rhinitis is made after an allergic cause is ruled out.
DNA extraction. For the various methods, see Nucleic acid methods. DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods. The first isolation of DNA was done in 1869 by Friedrich Miescher. Currently it is a routine procedure in molecular biology or forensic analyses.
2. Digestion of DNA with a restriction endonuclease. Extracted DNA is treated with a restriction endonuclease, which is an enzyme that will cut double stranded DNA whenever a specific DNA sequence occurs. The enzyme most commonly used for forensic DNA analysis is HaeIII, which cuts DNA at the sequence 5'-GGCC-3'.maller DNA fragments move more rapidly through the pores of the gel matrix than larger DNA fragments. The result is a continuous separation of the DNA fragments according to size, with the smallest DNA fragments moving the greatest distance away from the origin.