1 10/14/2015 4:30 pm - 5:30 pm. 2  BRWN 4102. 3  Dissertation Seminar.  4  Design and Development of Non-Linear Optical Microscopy Data Acquisition and Analysis Techniques  by Shane Sullivan (Simpson Group).
DNA polymerase II (also known as DNA Pol II or Pol II) is a prokaryotic DNA-Dependent DNA polymerase encoded by the PolB gene.DNA Polymerase II is an 89.9-kDa protein and is a member of the B family of DNA polymerases.It was originally isolated by Thomas Kornberg in 1970, and characterized over the next few years.NA Pol II is not the most studied polymerase so there are many proposed functions of this enzyme which are all likely functions but are ultimately unconfirmed. These are: repair of DNA damaged by UV irradiation, replication restart in UV-irradiated E. coli, adaptive mutagenesis, and long-term survival.
During replication, this can remove primers ahead of the polymerizing function of the polymerase. See Figure 3 . Figure 3. Another intimate function of DNA polymerase I (and of the other forms of DNA polymerase found in E. coli) is the 3â² to 5â² exonuclease activity. This activity can deâpolymerize DNA starting from the newly synthesized end. Imagining why DNA polymerase would have an activity that opposes the action of the enzyme is a little difficult. The 3â² to 5â² exonuclease activity serves an editing function to ensure the fidelity of replication. Suppose DNA polymerase were to make a mistake and add a T opposite a G in the template strand. When the enzyme begins the next step of polymerization, the T is not properly paired with the template.