Thoracic outlet syndrome can result from injury, disease, or a congenital problem, such as an abnormal first rib. It is more common in women than in men, and poor posture and obesity can aggravate the condition. Psychological changes are often seen in patients with thoracic outlet syndrome.
Excess detergent can also be removed from a proteinsolution with Bio-Beads Â® SM-2 adsorbent.Alternatively, the Bio-Rad DC(detergent compatible) Protein Assaycan be used. The DCProtein Assay is a modified Lowry assay whichworks in the presence of 1% ionic or nonionic detergent. This two-step method is ready to evaluate in just 15 minutes, and each kit willassay up to 500 samples with the standard method, or 10,000 sampleswith the microplate method.3.
Gel Retardation Assay (Molecular Biology). The gel retardation assay, also known as the bandshift assay or electrophoretic mobility shift assay (EMSA), is used to detect DNA-binding proteins in crude cell extracts and to study the binding activity of purified DNA-binding proteins. It is also applicable to the study of RNA-protein interactions. The basis of the assay is that protein-DNA complexes remain intact when gently fractionated by gel electrophoresis and migrate as distinct bands more slowly than the free DNA fragment.