Here, we have investigated the effect of growth-temperature on both the activity and stability of the inclusion bodies formed by a point-mutant of AÎ²42 Alzheimer peptide, using green fluorescent protein as a reporter. The activity in the aggregates inversely correlates with the temperature.
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Tipping. 1  Hotels Not expected. 2  Restaurants Not expected; 5% to 10% in smart restaurants or if you're very satisfied. 3  Guides A few dollars on day trips is sufficient, more for longer trips if the service is good. 4  Taxis Not necessary, but a little extra is appreciated, especially at night. 5  Bars Never expected.