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Those of us who are deficient and start on a vitamin D supplement usually take five to six weeks to reach the recommended level of vitamin D. If you're in doubt, it's always best to have a chat with your GP who might arrange a blood test. The B vitamins and vitamin C are all water soluble.
2 How long does it take a vitamin B12 injection to take effect? Dr. Joel Gallant Dr. Gallant. Take effect?: I'm not sure what you mean by 'take effect.' the only indication for vitamin B12 injection is for people with severe B12 deficiency or those who are unable to absorb B12 from their diet.