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No, not necessarily, but it can cause a minor issue. iPhones use a Lithium-Ion battery for its power, using while it's charging won't necessarily ruin the battery, but if you're using the phone while the battery is in the charging process, it can cause the phone to think it has more power than it actually has.
Charge the batteries using your chosen method. If charging the battery/batteries inside your cell phone, turn the phone off before charging unless otherwise directed by any manufacturerâs instructions. Charge your cell phone for 12 to 24 hours depending on the type of battery your phone uses and/or the manufacturerâs instructions. For example, NiMH (Nickel Metal Hydride) batteries require a longer charging period than Li-ION (Lithium Ion) batteries.