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Gene targeting is a genetic technique that uses homologous recombination to change an endogenous gene. The method can be used to delete a gene, remove exons, add a gene, and introduce point mutations. Gene targeting can be permanent or conditional. Conditions can be a specific time during development / life of the organism or limitation to a specific tissue, for example. Gene targeting requires the creation of a specific vector for each gene of interest. However, it can be used for any gene, reg
The unique colony holding the gene sequence of interest is identified by comparing the main plate with bacterial colonies, the nitro cellulose membrane and the X-ray film. The identified colony carrying gene sequence of interest can then be extracted from the plate and grown as a step to amplify the gene of interest. Using expression vectors in creating cDNA library makes the screening of the cDNA library much easier which is done with the help of specific antibodies.