The city of Boston is certainly familiar with the feeling of angst, and its fans have been known to let their emotions rain down upon the sports figures they despise the most. It's a routine passed down through generations.
Brian Orser. Brian Orser (Order of Canada) (born December 18, 1961 in Belleville, Ontario, Canada) is a Canadian retired professional figure skater. He is one of the most accomplished skaters in Canada's history, with eight national titles, two Olympic medals, and a world title to his credit.
By complementing repA- ori+ plasmids with the repA+ ori- plasmid, the left end of oriR was localized at position 1,424. Therefore, the oriR sequence, localized within a region of 188 base pairs, is separate from the repA gene. A hybrid plasmid carrying the 206-base-pair segment between positions 1,406 and 1,611 also replicates in a polA- cell extract when the repA function is supplied in trans. Removal of an additional 66 base pairs (positions 1,406-1,471) inactivates the function of the minimal oriR segment. PMCID: PMC390076 PMID: 6316326 [PubMed - indexed for MEDLINE]