Overnight test: After determination of baseline serum cortisol, 8 mg (typically) PO between 11:00 pm and midnight; cortisol level tested between 8:00 and 9:00 AM on following morning. IV test: After determination of baseline serum cortisol, 1 mg/hr by continuous IV infusion for 5-7 hours.
Simple: Surgical closure of a superficial wound, requiring single layer suturing of the. skin (epidermis, dermis, or subcutaneous tissue). Intermediate: 1) Surgical closure of a wound requiring closure of one or more of the. deeper subcutaneous tissue and non-muscle fascia layers in addition to the skin.
Sticky ends refers to one outcome of cleavage of DNA with a restriction enzyme. In this case, it refers to an overhang which is produced when restriction enzymes cut the two DNA strands four base pairs from each other, creating a four-base 5' overhang in one molecule and a complementary 5' overhang in the other.