Certain retirement accounts allow you to accelerate your savings rate after age 50. If you have a traditional or Roth IRA, you can make extra contributions. You can put away up to $6,500 per year, as of 2013. You can contribute an additional $5,500 to your 401(k), above the $17,500.
It would be dangerous to incubate (keep and grow) cultures at temperatures close to body temperature (37Â°C) because doing so might allow the growth of pathogens harmful to health. So the maximum temperature used in school and college labs is 25Â°C.
Set up T-175 flasks (as many as needed) of WEHI-3 cells at 10 5 cells/ml. 2. Incubate for 2-4 days without feeding until the cell concentration is 6 x10 5 to 1 x10 6 cells/ml. If the cells have not reached the target concentration by day 4, use a different flask.hat final cell concentration is 5 x10 5 cells/ml. 3. For each batch tested, add 50 Î¼l of 40% WEHI-3 CM into a well of a 96 well flat-bottomed plate. Place plate in incubator at 37Â°C, 5% CO 2 to equilibrate for 1 hour. 4. Add 50 Î¼l of CellTiter/CMLESS cell suspension from step 2 to each well and shake gently to mix.