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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
1 How long does it take for nicotine to leave your body (urine and blood stream) after you stop smoking?  Stop smoking at 11:59pm on 03-04-16, I have a blood,urine and mouth test for nicotine. The test is between 3-14/1 more Stop smoking at 11:59pm on 03-04-16, I have a blood,urine and mouth test for nicotine.