Now, a root-locus line starts at every pole. Therefore, any place that two poles appear to be connected by a root locus line on the real-axis, the two poles actually move towards each other, and then they break away, and move off the axis. The point where the poles break off the axis is called the breakaway point.
Ethidium bromide is an intercalcating dye, which means it inserts itself between the bases that are stacked in the center of the DNA helix. One ethidium bromide molecule binds to one base. As each dye molecule binds to the bases the helix is unwound to accommodate the strain from the dye.Closed circular DNA is constrained and cannot withstand as much twisting strain as can linear DNA, so circular DNA cannot bind as much dye as can linear DNA.ne ethidium bromide molecule binds to one base. As each dye molecule binds to the bases the helix is unwound to accommodate the strain from the dye. Closed circular DNA is constrained and cannot withstand as much twisting strain as can linear DNA, so circular DNA cannot bind as much dye as can linear DNA.
But they all consist of 4 nucleotides 2 made of purine bases which are adenine and guanine and 2 pyrimidine bases which are thymine and cytosine. The purine based nucleotides always pair with their base pair pyrimidine based nucleotide.In DNA Adenine is paired with thymine and guanine pairs with cytosine.here are four nucleotides that make up DNA, each defined by the  nitrogenous bases they contain. They are named adenine, thymine,  guanine and cytosine.