Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
The cells that decolorize easily are gram negative, those that hold the primary stain are gram positive. Negative staining is used to show better morphology(shape) and size of cells, as well as being used in hard to stain bacteria such as coccobacilli.