Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
To stain a capsule of a cell by Manevalâs staining method. 1  1 % Congo red solution. 2  Manevalâs stain. 3  Capsulated cell culture. 4  Take a loopful of capsulated cell suspension on a clean grease free slide.  Add a drop of 1 % congo red solution in the suspension and then spread it gently on the slide to form a smear.