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Many legumes have root nodules that provide a home for symbiotic nitrogen-fixing bacteria called rhizobia. This relationship is particularly common in nitrogen-limited conditions. The Rhizobia convert nitrogen gas from the atmosphere into ammonia, which is then used in the formation of amino acids and nucleotides .
Polygonum cuspidatum, known by the common name Japanese knotweed, is a tall, stout herbaceous perennial (Seiger, 2005) and is a member of the Polygonaceae (Seiger, 2005). In China it is referred to as Hu Zhanz. Japanese knotweed is also known under the scientific names Fallopia japonica and Reynoutria japonica. The leaves are alternately arranged and are oval with pointed tips and a truncate base. They measure approximately 15 cm long by 7.5-10 cm wide (Remaley, 2005).