M1, M3 and M5 receptors cause the activation of phospholipase C, generating two secondary messengers (IP3 and DAG) eventually leading to an intracellular increase of calcium, while M2 and M4 inhibit adenylate cyclase, thereby decreasing the production of the second messenger cAMP.
Keep your dog healthy and parvo-free with these 8 tips: Make sure your dog is properly vaccinated. Puppies should receive their first vaccines at 6-8 weeks of age; boosters should be administered at three-week intervals until the puppy is 16 weeks of age, and then again at one year of age. Previously vaccinated adult dogs need boosters every year.
At 6-8 weeks of age puppies should receive their first vaccination; this is temporary and needs to be followed up with another one at 12 weeks.