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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
A fluorescence microscope is much the same as a conventional light microscope with added features to enhance its capabilities. 1  The conventional microscope uses visible light (400-700 nanometers) to illuminate and produce a magnified image of a sample.