Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
Crystal violet - stains cell walls purple when combined with a mordant. This stain is used in Gram staining. DAPI - a fluorescent nuclear stain that is excited by ultraviolet light, showing blue fluorescence when bound to DNA. DAPI can be used in living of fixed cells.