Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
. Water is used to decolorize. the cells; as the endospores are resistant to staining, the endospores are equally resistant to de-staining and will retain the primary dye while the vegetative cells will lose the stain.The addition of a counterstain or secondary stain (safranin) is used to stain the decolorized vegetative cells.hen vegetative cells are subjected to environmental stresses such as nutrient deprivation they eventually die. However, some bacteria such as the Bacillus spp. and the Clostridium spp can circumvent the problems associated with environmental stress by forming. endospores. .