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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
The Concept of Magnification. A simple microscope or magnifying glass (lens) produces an image of the object upon which the microscope or magnifying glass is focused. Simple magnifier lenses are bi-convex, meaning they are thicker at the center than at the periphery as illustrated with the magnifier in Figure 1.