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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
The Stress Response. Cortisol is a chemical hormone produced by your body to manage stress. The stress can be physical, mental and emotional. Cortisol continues to be released when the stress continues. Effects of Cortisol.