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TRH stimulation test. Prior to the availability of sensitive TSH assays, thyrotropin releasing hormone (TRH) stimulation tests were relied upon for confirming and assessing the degree of suppression in suspected hyperthyroidism. Typically, this stimulation test involves determining basal TSH levels and levels 15 to 30 minutes after an intravenous bolus of TRH.
Human thyroid stimulating hormone (hTSH) or thyrotropin is a glycoprotein synthesized by the basophilic cells (thyrotropes) of the anterior pituitary. hTSH is composed of two non-covalently linked subunits designated alpha and beta.