Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
Capsule Stain. 1  Place a drop of Enterobacter aerogenes, grown for two days in skim milk broth, onto a slide. 2  Spread across the slide, allow to air dry. 3  Cover smear with crystal violet for 1 minute, rinse with either water or 20% copper sulfate. 4  Blot dry and observe under oil (100X).