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In metaphase, sister chromatids align along the metaphase plate at right angles to the cell poles. In anaphase, sister chromatids separate and begin moving toward opposite ends of the cell. Once the paired sister chromatids separate from one another, each chromatid is considered a single-stranded, full chromosome. In telophase and cytokinesis, separated sister chromatids are divided into two separate daughter cells. Each separated chromatid is referred to as a daughter chromosome.
What forms to help attach and move chromatids to the opposite poles of the cells? The mitotic spindle fibers formed of tubulin subunits. What is the cell structure that joins two sister chromatids of a chromosome? The cell structure that joins two sister chromatids into one single  chromosome is called the centromere. This is at the center of the  sister chromatids.