o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
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