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DNA-gyrase-catalyzed supercoiling. Replicating DNA generates âpositiveâ supercoils, depicted at the bottom of the diagram, as a result of rapid rotation of the DNA at the replication fork. DNA gyrase can nick and close phosphodiester bonds,
The following is a list of major DNA replication enzymes that participate in the replisome: Enzyme Function in DNA replication Also known as helix destabilizing enzyme. Unwinds the DNA double DNA Helicase helix at the Replication Fork. Builds a new duplex DNA strand by adding nucleotides in the 5' to 3' DNA Polymerase direction. Also performs proof-reading and error correction.