o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
You will still be able to legally drive as long as you renew on or before the expiration date. Your regular Class D Minnesota driver's licenses will expire every 4 years on your birthday. If you're under 21 years old, it will expire on your 21st birthday. Your Minnesota provisional license will expire 2 years from date of your application, not on your birthday. NOTE: If you're turning 21 years old, you should NOT renew your license until 3 weeks prior to your 21st birthday.