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Definition of LUCIFERASE. : an enzyme that catalyzes the oxidation of luciferin. ADVERTISEMENT. an enzyme that catalyzes the oxidation of luciferin.
Light is produced by converting the chemical energy of luciferin oxidation through an electron transition, forming the product molecule oxyluciferin. Firefly luciferase, a monomeric 61kDa protein, catalyzes luciferin oxidation using ATP-Mg 2+ as a cosubstrate.In the conventional assay for luciferase, a flash of light is generated that decays rapidly after the enzyme and substrates are combined.ight is produced by converting the chemical energy of luciferin oxidation through an electron transition, forming the product molecule oxyluciferin. Firefly luciferase, a monomeric 61kDa protein, catalyzes luciferin oxidation using ATP-Mg 2+ as a cosubstrate.