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Definition of LUCIFERASE. : an enzyme that catalyzes the oxidation of luciferin. ADVERTISEMENT. an enzyme that catalyzes the oxidation of luciferin.
One is a luciferin, or a light-producing substance. The other is a luciferase, or an enzyme that catalyzes the reaction. In some cases, the luciferin is a protein known as a photoprotein, and the light-making process requires a charged ion to activate the reaction.Neurological, mechanical, chemical or as-yet-undiscovered triggers can start the reactions that create light.n some cases, the luciferin is a protein known as a photoprotein, and the light-making process requires a charged ion to activate the reaction. Neurological, mechanical, chemical or as-yet-undiscovered triggers can start the reactions that create light.