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Effects of substituents on the acidity of phenols: Existence of electron attracting group, (for example-NO2, -X, - NR, -CN, -CHO, -COOH) on the benzene ring increases the acidity of phenol since it enables the ring to draw much more electrons from the phenoxy oxygen and so releasing easily the proton.
As you increase the pH of the buffered solution the volume of oxygen produced is increased. Conclusion Discussion The effect of pH on the activity of catalase An enzyme found in many different tissues,catalayses the breakdown of hydrogen peroxide into water and oxygen. Hydrogen peroxide is a toxic substance that can be formed during aerobic respiration and catalase removes this product.