o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
How Long Does an Accident Stay on Your Record? Posted on Nov 10, 2015 If you have just been in a car accident or ticketed for a traffic violation, you may have heard conflicting information regarding how long the accident or ticket will stay on your record and how it will affect your insurance premium. The short answer is that the accident or ticket will stay on your record [â¦]