o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H 2 SO 4 and read at 492 nm.
If a person is issued a ticket for an accident in Minnesota, it  will stay on his or her driving record for five years. However, if  the accident was alcohol related, depend â¦ ing on the charge, it can  stay longer. 1 person found this useful.
Your driving habits reflect on your driver abstract. Traffic violations and collisions could be a negative influence on a record. A traffic ticket could stay on your driving record for 3 years from the date of conviction. An at-fault car accident may stay on your driver abstract for at least 6 years and up to 10 years.