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In metaphase, sister chromatids align along the metaphase plate at right angles to the cell poles. In anaphase, sister chromatids separate and begin moving toward opposite ends of the cell. Once the paired sister chromatids separate from one another, each chromatid is considered a single-stranded, full chromosome. In telophase and cytokinesis, separated sister chromatids are divided into two separate daughter cells. Each separated chromatid is referred to as a daughter chromosome.
A sister chromatid refers to either of the two identical copies (chromatids) formed by the replication of a single chromosome, with both copies joined together by a common centromere.In other words, a sister chromatid may also be said as 'one-half' of the duplicated chromosome.A full set of sister chromatids is created during the synthesis (S) phase of interphase, when all the chromosomes in a cell are replicated.ollowing chromosomal replication, the blue chromosome is composed of two identical sister chromatids and the pink chromosome is composed of two identical sister chromatids.