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In metaphase, sister chromatids align along the metaphase plate at right angles to the cell poles. In anaphase, sister chromatids separate and begin moving toward opposite ends of the cell. Once the paired sister chromatids separate from one another, each chromatid is considered a single-stranded, full chromosome. In telophase and cytokinesis, separated sister chromatids are divided into two separate daughter cells. Each separated chromatid is referred to as a daughter chromosome.
It is produced from the duplication of the chromosome in the early stages of cell division. There are two forms of chromatids, sister or non-sister chromatids. Sister chromatids are two identical copies of a chromatid having the same genes and alleles. Non-sister chromatids are also called homologues having the same length, staining pattern, centromere position, as well as the same characteristics of genes at a particular loci.