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Definition of LUCIFERASE. : an enzyme that catalyzes the oxidation of luciferin. ADVERTISEMENT. an enzyme that catalyzes the oxidation of luciferin.
Luciferin (from the Latin lucifer, light-bringer) is a generic term for the light-emitting compound found in organisms that generate bioluminescence. Luciferins typically undergo an enzyme-catalysed oxidation and the resulting excited state intermediate emits light upon decaying to its ground state.