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Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent.Contents.thanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent. Contents.
I recommend stopping with about 50ul left and pipeting slowly so that if a DNA pellet is loose it will not get sucked up into the pipet tip. After removal of the majority of ethanol from the second 70% wash, spin samples again briefly (to 8,000 rpm) to bring ethanol from the sides down to the bottom of the tube.