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DNA-gyrase-catalyzed supercoiling. Replicating DNA generates âpositiveâ supercoils, depicted at the bottom of the diagram, as a result of rapid rotation of the DNA at the replication fork. DNA gyrase can nick and close phosphodiester bonds,
We begin by discussing. the conformations of (2) supercoiled DNA that is not replicat-. ing, the form that DNA adopts away from the fork. Next, we. discuss the three ways in which replicating DNA may differ from. nonreplicating DNA: precatenanes, (1) supercoiled DNA, and. the four-way junction at stalled forks.