Students learn about push-pull factors and corresponding population mobility patterns. This lesson plan was given the 2006 Geography Excellence in Media (GEM) Award by the National Council for Geographic Education (NCGE).
ED50V10 (Readme) is an Excel add-in for calculating IC50/EC50 values. Input your data in the left columns, and your results will be shown in the right half of the Excel table. To calculate IC50, input 50 in the INTERPOLATE... table (highlighted in blue), the result will be shown on the right (highlighted in green).inear Regression. The simplest estimate of IC50 is to plot x-y and fit the data with a straight line (linear regression). IC50 value is then estimated using the fitted line, i.e., Y = a * X + b, IC50 = (0.5 - b)/a. Log transformation. Frequently, linear regression is not a good fit to dose-response data.
Hereâs how you calculate TPM: 1  Divide the read counts by the length of each gene in kilobases. This gives you reads per kilobase (RPK). 2  Count up all the RPK values in a sample and divide this number by 1,000,000. This is your âper millionâ scaling factor. 3  Divide the RPK values by the âper millionâ scaling factor. This gives you TPM.