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Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent.Contents.thanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent. Contents.
Making the world better, one answer at a time. An essential cofactor for the DNA polymerase in PCR is Magnesium chloride. Its concentration must be optimized for every primer:template system. Many components of the reaction bind magnesium ion, including primers, template, PCR products and dNTPs.The main 1:1 binding agent for magnesium ion is the high concentration of dNTPs in the reaction.ypically, to start the optimization process, 1.5 mM magnesium chloride is added to PCR in the presence of 0.8 mM total dNTPs. This leaves about 0.7 mM free magnesium for the DNA polymerase.