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Definition of LUCIFERASE. : an enzyme that catalyzes the oxidation of luciferin. ADVERTISEMENT. an enzyme that catalyzes the oxidation of luciferin.
In a firefly's tail, you'll find two chemicals: luciferase and luciferin. Luciferin is heat resistant, and it glows under the right conditions. Luciferase is an enzyme that triggers light emission. ATP, a chemical within the firefly's body, converts to energy and initiates the glow.