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Primer sequences are determined from known sequence since there must be a match to the region of DNA to be amplified. PCR amplification requires 2 primers that determine the region of sequence amplified in the forward and reverse direction.ikewise, the reverse primer is designed from the complimentary strand. PCR is exponential amplification in which the newly generated PCR fragment from one cycle also acts as a template for the next cycle (Figure 1A). Amplification of DNA for Sanger sequencing differs from PCR in that a single primer is used.
Amplification means increasing the amplitude (voltage or current) of a time-varying signal by a given factor, as shown here. In this example the amplifier has a voltage gain of 3; that is at any instant v o = 3 v i {\displaystyle v_{o}=3v_{i}\,}. An amplifier, electronic amplifier or (informally) amp is an electronic device that can increase the power of a signal (a time-varying voltage or current). An amplifier functions by using electric power from a power supply to increase the amplitude of the voltage or current signal.