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Altogether 786 isolates from time interval 2008-2010 were grouped into 90 PCR ribotypes and eleven of them were shared among all host types and the environment. Ribotypes that were most common in humans were also present in water and different animals (014/020, 002, 029). Interestingly, non-toxigenic isolates were very common in the environment (30.8%) in comparison to humans (6.5%) and animals (7.7%). A high degree of similarity was observed for human and animal isolates with PFGE.
Exercise A: Isolation of an Unknown Bacterium from Soil Experiment 1: Primary Cultures from Soil Extracts Objective: In this experiment students will obtain soil samples, and isolate colonies on primary culture plates using the T-streak.oil Bacteria 85 Experiment 3: Identification of an Unknown Bacterium from Secondary Cultures Objective: In this experiment students will record colony characteristics of the three pure cultures from their three secondary plates.