Located near the center of the US population, Northwest Arkansas is, quite simply, in the middle of everywhere. There are several Midwestern markets within a half-day's drive, including Kansas City, St. Louis, Dallas-Fort Worth, Little Rock, Tulsa, and Oklahoma City.
Restriction enzyme. A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.
Restriction Enzymes Cut DNA Molecules at Specific Sequences. To clone specific DNA fragments in a plasmid vector, as just described, or in other vectors discussed in later sections, the fragments must be produced and then inserted into the vector DNA.As noted in the introduction, restriction enzymes and DNA ligases are utilized to produce such recombinant DNA molecules.hen such a vector is treated with a restriction enzyme that recognizes a recognition sequence in the polylinker, it is cut at that sequence, generating sticky ends. In the presence of DNA ligase, DNA fragments produced with the same restriction enzyme will be inserted into the plasmid (Figure 7-8b).