There are three qualitative cytotoxicity tests commonly used for medical devices. The Direct Contact procedure is recommended for low density materials, such as contact lens polymers. In this method, a piece of test material is placed directly onto cells growing on culture medium. The cells are then incubated.
A muscle cramp is a strong, painful contraction or tightening of a muscle that comes on suddenly and lasts from a few seconds to several minutes. It often occurs in the legs. A muscle cramp is also called a charley horse. Nighttime leg cramps are usually sudden spasms, or tightening, of muscles in the calf. The muscle cramps can sometimes happen in the thigh or the foot. They often occur just as you are falling asleep or waking up.
1 Constant leg pain and Cramping leg pain and Blood clots (4 causes) 2  Constant leg pain and Cramping leg pain and Blood vessel symptoms (4 causes) 3  Constant leg pain and Cramping leg pain and Body symptoms (4 causes)  Constant leg pain and Cramping leg pain and Breath symptoms (4 causes)