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This can be demonstrated through the cross-streaking of Rhodococcus equi on sheep blood agar with another bacterium (i.e. A. haemolyticum, Staphylococcus aureus, and Corynebacterium pseudoteberculosis). The picture below shows some mechanisms of synergistic hemolysis.
Typically PEA agar is used to inhibit the common contaminants such as Escherichia coli and Proteus species. PEA agar may be prepared with and without 5% sheep blood supplement. PEA agar with 5% sheep blood is used to isolate most gram-positive and gram-negative anaerobes from enteric samples (12).It is used to inhibit facultative gram-negative rods, preventing Enterobacteriaceae from overgrowing the anaerobes, and inhibiting swarming of Proteus and Clostridium septicum (7,12).EA agar may be prepared with and without 5% sheep blood supplement. PEA agar with 5% sheep blood is used to isolate most gram-positive and gram-negative anaerobes from enteric samples (12).