Located near the center of the US population, Northwest Arkansas is, quite simply, in the middle of everywhere. There are several Midwestern markets within a half-day's drive, including Kansas City, St. Louis, Dallas-Fort Worth, Little Rock, Tulsa, and Oklahoma City.
Restriction enzyme. A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.