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Anaphylactic reactions are excluded by the presence of red plasma or urine, discordant pretransfusion and posttransfusion ABO blood types, or a positive direct antiglobulin (Coombs) test result. Demonstration of anti-IgA in a pretransfusion sample of the recipient's serum or plasma establishes the diagnosis.
The intial and post screen for a patient that has Delayed hemolytic Transfusion Reaction are. 1) Initial is antibody screening is negative and compatible cross-match. 2) Post is anitbody screening that is positive along with a positive DAT.