Located near the center of the US population, Northwest Arkansas is, quite simply, in the middle of everywhere. There are several Midwestern markets within a half-day's drive, including Kansas City, St. Louis, Dallas-Fort Worth, Little Rock, Tulsa, and Oklahoma City.
Restriction enzyme. A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.
A restriction map is a description of restriction endonuclease cleavage sites within a piece of DNA. Generating such a map is usually the first step in characterizing an unknown DNA, and a prerequisite to manipulating it for other purposes.Typically, restriction enzymes that cleave DNA infrequently (e.g. those with 6 bp recognition sites) and are relatively inexpensive are used to produce at a map.The DNA to be restriction mapped it usually contained within a well-characterized plasmid or bacteriophage vector for which the sequence is known.In fact, there are usually multiple known restriction sites immediately flanking the uncharacterized DNA, which facilitates making the map.he most straightforward method for restriction mapping is to digest samples of the plasmid with a set of individual enzymes, and with pairs of those enzymes.