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RNA polymerase. Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription.NA polymerase. Rna polymerase (RNAP or RNApol) is an enzyme that is responsible for making rna from a dna template. In all cells RNAP is needed for constructing rna chains from a dna template, a process termed transcription.
RNA is synthesized by RNA polymerase that reads one strand of DNA. RNA polymerase reads DNA 3' to 5'. When RNA is made, it is made 5' to 3'. Most polymerases have the 3' to 5' reading activity. The created RNA strand is identical to the coding strand of DNA, which is also in the orientation of 5' to 3'.