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Definition A buffer solution is one which resists changes in pH when small quantities of an acid or an alkali are added to it. Acidic buffer solutions An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts-often a sodium salt.
The pH of concentrated stock. solutions may change on dilution. For example, the pH value of an 100 mM sodium phosphate buffer increases from 6.7 to. 6.9 with 10fold dilution and to 7.0 with 100fold dilution (Tipton & Dixon 1979). The pH value of a Tris solution decreases. by 0.1 pH units per 10fold dilution.