The Sanger method relies upon a variation of the replication process described above in order to determine the sequence of nucleotides in a segment of DNA. Before Sanger sequencing can begin, however, researchers must first make many copies of, or amplify, the DNA segment they wish to sequence.
15221 zip code is located in west Pennsylvania. 15221 zip code is part of Allegheny County. 15221 zip code has 6.15 square miles of land area and 0.01 square miles of water area.
15204 zip code is located in west Pennsylvania. 15204 zip code is part of Allegheny County. 15204 zip code has 1.86 square miles of land area and 0.09 square miles of water area. As of 2010, the total 15204 zip code population is 8,329, which has shrunk 12.34% since 2000.