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Chemical protein synthesis, which relies on chemoselective peptide ligation methods, such as native chemical ligation (NCL), offers complete control of the atomic composition of the polypeptide sequence, making it advantageous over molecular biology approaches in some areas of research (Fig. 1).
Solid-phase synthesis of peptides. Solid-phase synthesis is the most common technique for peptide synthesis. Usually, peptides are synthesized from the carbonyl group side (C-terminus) to amino group side (N-terminus) of the amino acid chain in this method, although peptides are synthesised in the opposite direction in cells.