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Chemical protein synthesis, which relies on chemoselective peptide ligation methods, such as native chemical ligation (NCL), offers complete control of the atomic composition of the polypeptide sequence, making it advantageous over molecular biology approaches in some areas of research (Fig. 1).
In this method, the protein concentration is determined by the absorbance at 205 nm in which the peptide bonds are analyzed directly. DNA or RNA purity can also be determined by measuring the absorbance ratios of 260 to 280 nm. This is because the nucleic acids that make up DNA and RNA absorb strongly at 260 nm.