A less hefty penalty of 0.5 to 1 percent of your unpaid taxes per month applies to taxpayers who file by the deadline, but owe taxes and don't pay up. Even if you can't afford your taxes now, it's best to at the very least file your tax return on time.Those who both file late and fail to pay for the taxes they owe are charged a maximum penalty of 5 percent of their unpaid taxes for every month the bill is late. less hefty penalty of 0.5 to 1 percent of your unpaid taxes per month applies to taxpayers who file by the deadline, but owe taxes and don't pay up. Even if you can't afford your taxes now, it's best to at the very least file your tax return on time.
Methods. Catechol oxidase is the enzyme responsible for the browning of fruit. It is easy to prepare from a number of different sources â bananas are particularly good â and the reaction is readily followed using a colorimeter.nvestigating the effect of pH. A reaction mix containing 0.01M catechol should give a good response at 25Â°C. (0.3mls 0.1M catechol + 2.6mls buffer added to 0.1mls enzyme extract in a standard cuvette). The initial straight line portion of the graph of absorbance against time gives V i â the initial rate of reaction.
Apples and pears also contain catechins, with 7 and 4 milligrams, respectively, in 100 grams. Among vegetables, beans are good sources of catechins. For example, 100 grams of fava beans provide about 20 milligrams of catechins. Red table wine is an excellent source of several flavonoids, including catechins, with about 10 milligrams of these compounds in 100 grams, or 3.5 ounces of liquid.