Replaced blower fan resistor today without removing dash. Dealer wanted 10 hours labor to replace resistor. Took me two hours, next one could do in hour. 4 of 5 fan screws you can get to without problems. For 5th screw which requires removal of dash, I just pulled fan down and mount broke right off.
1 Answer 1. If I understand the nomenclature correctly, an R plasmid is just any plasmid containing an antibiotic (R)esistance gene (eg. Amp, Kan, Cm, etc.). It's a bit of an outdated name from when people didn't know how exactly the plasmids conferred such resistance.
In their simplest form, plasmids require a bacterial origin of replication, an antibiotic resistance gene, and at least one unique restriction enzyme recognition site. These elements allow for the propagation of the plasmid within bacteria, while allowing for selection against any bacteria not carrying the plasmid. Self-replicating-Once you have constructed a plasmid, you can make an endless number of copies of the plasmid by growing the plasmid in bacteria. 2  Stable-Plasmids are stable long-term either as purified DNA or within bacteria (as glycerol stocks).