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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
The mother cellâs DNA is copied during interphase. During mitosis the chromosomes condense from long strands to highly coiled structures. The two copies of each DNA strand, called sister chromatids, are physically attached to one another. The chromosomes are moved to the center of the cell and split apart in a highly coordinated fashion.