Replaced blower fan resistor today without removing dash. Dealer wanted 10 hours labor to replace resistor. Took me two hours, next one could do in hour. 4 of 5 fan screws you can get to without problems. For 5th screw which requires removal of dash, I just pulled fan down and mount broke right off.
1 Answer 1. If I understand the nomenclature correctly, an R plasmid is just any plasmid containing an antibiotic (R)esistance gene (eg. Amp, Kan, Cm, etc.). It's a bit of an outdated name from when people didn't know how exactly the plasmids conferred such resistance.
The pBR322 plasmid contains a gene that allow the bacteria to be resistant to the antibiotics tetracycline and amipicillin. To use pBR322 plasmid to clone a gene, a restriction endonuclease first cleaves the plasmid at a restriction site.pBR322 plasmid contains three restriction sites: PstI, SalI and ecoRI. The first two restriction sites are located within the gene that codes for ampicillin and tetracycline resistance, respectively.owever, plasmids are not limited to bacteria; they are also present in some eukaryotes (e.g., circular, nuclear plasmids in Dictyostelium purpureum). A plasmid is a circular, double stranded DNA that is usually found in bacteria (however it does occur in both eukarya and prokarya).