Replaced blower fan resistor today without removing dash. Dealer wanted 10 hours labor to replace resistor. Took me two hours, next one could do in hour. 4 of 5 fan screws you can get to without problems. For 5th screw which requires removal of dash, I just pulled fan down and mount broke right off.
1 Answer 1. If I understand the nomenclature correctly, an R plasmid is just any plasmid containing an antibiotic (R)esistance gene (eg. Amp, Kan, Cm, etc.). It's a bit of an outdated name from when people didn't know how exactly the plasmids conferred such resistance.
Just kidding. For our purposes, we mainly use plasmids as cloning vectors. And as such, a good plasmid has the following: 1  oriC, an origin of replication. 2  Gotta start making new plasmid somewhere. a multiple cloning site (MCS) inside a scorable marker. 2  The MCS allows us to cut the plasmid, insert new DNA, and re-ligate; the scorable marker allows us to see if the plasmid does indeed have an insert, because the insert will disrupt expression of the marker.