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Equally important and coupled with DNA methylation is DNA demethylation, the removal of a methyl group. The demethylation process is necessary for epigenetic reprogramming of genes and is also directly involved in many important disease mechanisms such as tumor progression.
Once the two strands are separated a small piece of RNA, called an RNA primer, is attached to the DNA by an enzyme called DNA primase. These primers are the beginnings of all new DNA chains since the enzyme responsible for the copying of the DNA, DNA polymerase, can not start from scratch. It is a self-correcting enzyme and copies the DNA template with remarkable fidelity. The DNA polymerase can only read in the 3' to 5' direction.