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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Actively dividing eukaryote cells pass through a series of stages known collectively as the cell cycle: two gap phases (G1 and G2); an S (for synthesis) phase, in which the genetic material is duplicated; and an M phase, in which mitosis partitions the genetic material and the cell divides.G1 phase.itosis is a form of eukaryotic cell division that produces two daughter cells with the same genetic component as the parent cell. Chromosomes replicated during the S phase are divided in such a way as to ensure that each daughter cell receives a copy of every chromosome.