mycobacteria and fungi: thick cell walls, difficult to lyse than bacteria and parasite. GP bacteria have thick cell walls than GN bacteria and may require more rigorous cell lysis condition. mycoplasma lacks a cell wall, care must betaken to avoid spontaneous lysis of cells and loss of NA.
The Average Cost of a Bachelor's Degree. The average annual tuition and fees for a four-year bachelor's degree in the United States is $8,893 for in-state attendees of public colleges, $22,203 for out-of-state attendees of public colleges, and $30,094 for private nonprofit colleges.
US News offers this breakdown (tuition plus other expenses) of the average cost of 2 and 4 year schools. The cost of a master's degree varies widely. Some schools charge as little as $5,000 to $10,000 per year, while others charge up to $45,000 per year.