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A northern blot is a laboratory method used to detect specific RNA molecules among a mixture of RNA. Northern blotting can be used to analyze a sample of RNA from a particular tissue or cell type in order to measure the RNA expression of particular genes.
Southwestern blotting is a method that is used to isolate, identify, and characterize DNA-binding proteins by their ability in binding to specific oligonucleotide probes [ 44, 45 ]. Many of the DNA-binding proteins in the cell need to be isolated individually and characterized to define the gene function [ 44 ].