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Since the initial observation that GTP reacted with a soluble protein factor(s), present in an ammonium chloride extract of E. coli ribosomes, to form a complex that was retained on a nitrocel- lulose filter (11), it has been shown that both transfer factors, Ts and Tu, are required to form the GTP complex (6,8).
Activation of glucose for glycogen synthesis. Both glycogenin and glycogen synthase use an activated form of glucose, UDP-glucose, which is formed from glucose-6-phosphate in two steps.Phosphoglucomutase first transforms glucose-6-phosphate to glucose-1-phosphate (1), which is then converted to UDP-glucose (2).oth glycogenin and glycogen synthase use an activated form of glucose, UDP-glucose, which is formed from glucose-6-phosphate in two steps. Phosphoglucomutase first transforms glucose-6-phosphate to glucose-1-phosphate (1), which is then converted to UDP-glucose (2).