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Since the initial observation that GTP reacted with a soluble protein factor(s), present in an ammonium chloride extract of E. coli ribosomes, to form a complex that was retained on a nitrocel- lulose filter (11), it has been shown that both transfer factors, Ts and Tu, are required to form the GTP complex (6,8).
I. Catabolic and Anabolic Reactions. a. Metabolism is the sum of the chemical reactions in an organism. i. Catabolism refers to the energy-releasing processes (degradative, hydrolysis reactions, exergonic).TP is generated by the phosphorylation of ADP. iii. Substrate-level phosphorylation: 1. This is the transfer of a high-energy P i from a phosphorylated compound directly to ADP. Compound-P i + ADP ï  Compound + ATP iv.