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A northern blot is a laboratory method used to detect specific RNA molecules among a mixture of RNA. Northern blotting can be used to analyze a sample of RNA from a particular tissue or cell type in order to measure the RNA expression of particular genes.
To detect the antigens blotted on a nitrocellulose membrane with the use of an antibody. PRINCIPLE: Western blotting (also known as protein blotting or immunoblotting) is a rapid and sensitive assay for detective and characterization of proteins.estern blotting (also known as protein blotting or immunoblotting) is a. rapid and sensitive assay for detective and characterization of proteins. Western. blotting technique exploits the inherent specificity by polyclonal or monoclonal. antibodies.