DO AHEAD: Brisket is best shortly off the grill, but you can still get good results smoking it up to 3 days ahead. Let cool for an hour before wrapping in foil and chilling. To serve, reheat meat, still wrapped, in a 325-degree oven until warmed through.
Template documentation should cover: The basic purpose of the template: what it does and if it is not immediately obvious, why it needs to be done. If there are other templates with similar names or purposes, it's a good idea to mention those, in order to reduce the chance of the wrong one being used.
The template is the DNA that contains the target you want to amplify. The primers are synthetic DNA molecules whose sequence matches a region flanking the target you are trying to amplify. It is possible nowadays, to chemically synthesize DNA molecules of any given base sequence, to use as primers.o make primers of the correct sequence that will bind to the template DNA, it is necessary to know a little bit of the template sequence, on either side of the region of DNA you wish to amplify. DNA polymerase and dNTPs are commercially available from biotechnology supply companies.