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Since the initial observation that GTP reacted with a soluble protein factor(s), present in an ammonium chloride extract of E. coli ribosomes, to form a complex that was retained on a nitrocel- lulose filter (11), it has been shown that both transfer factors, Ts and Tu, are required to form the GTP complex (6,8).
In most cases these amino acids can be synthesize by one step reactions from central metabolites. They are simple in structure and their synthesis is also straight forward. Glutamate can by synthesized by the addition of ammonia to a-ketoglutarate. Figure 1 - The synthesis of glutamate. Glutamine is made by the addition of another ammonia molecule to glutamate. Figure 2 - Synthesis of glutamine. The rest of the simple reactions involve transfer of the amino group (transamination) from glutamate or glutamine to a central metabolite to make the required amino acid. Aspartate is synthesize by the transfer of a ammonia group from glutamate to oxaloacetate. Figure 3 - The synthesis of aspartate.