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A: If your room temperature goes above about 80 degrees Fahrenheit, you would be safer to keep your insulin in the refrigerator. Q: I use an insulin pen. I have heard I should keep my pen at room temperature for only two weeks. But insulin in vials can be kept at room temperature up to 28 days.
An unopened vial of NovoLog insulin is good until the expiration date (it is best stored in the refrigerator). An opened vial is good for 28 days whether or not it is refrigerated; room temperature insulin is better tolerated (less burning sensation with injection) than refrigerated insulin. Add an answer | Read more about Diabetes.