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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
There are two forms of this assay: (1) the direct ELISA employs monoclonal antibodies to detect the presence of a particular antigen in a sample; (2) the indirect ELISA is used to determine the presence of a specific antibody (e.g., HIV antibodies) in a specimen such as serum.