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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
Sandwich ELISA, Highly Sensitive. Sandwich ELISA is a less common variant of ELISA, but is highly efficient in sample antigen detection. Moreover, many commercial ELISA pair sets are built on this sanwich ELISA. The sandwich ELISA quantify antigens between two layers of antibodies (i.e. capture and detection antibody).andwich ELISA Protocol is shown in a different section. Sandwich ELISA is a common tool to diagnose Influenza, e.g. H5N1 (Avian Flu) Hemagglutinin ELISA kit. In addition, a description of the application of sandwich ELISA to home pregnancy test can be found here.