Like humans and animals, plants need both water and nutrients (food) to survive. Most all plants use water to carry moisture and nutrients back and forth between the roots and leaves. Water, as well as nutrients, is normally taken up through the roots from the soil.
So, for example, a 3-month old puppy should be able to hold its urine for approximately 4 hours in a pinch. This means that if you have a properly toilet trained 4-month-old puppy that, theoretically, can hold its urine for 5 hours, and you shut that puppy in a crate for 6 or 7 hours, you are courting disaster.
Best Answer: There is no real answer to your question, as much depends on the breed, physiology and size of your dog. My Afghan hounds can hold it through the night by 13 weeks(and most of them even earlier) ..But, my tiny Chihuahua couldn't until 2 years! However, he wasn't pee-pad trained. He woke me at 4 am to take him out..Gradually, it became, 5 am..6am, etc..Now he will usually hold it until I get up.