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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
Unfortunately there are limitations of IgG testing: 1  High levels of IgG can be bad (causing an immune response and a release of mediators thereby symptoms). 2  IgG only plays a minor role in IBS, migraine, fibromyalgia. 3  IgG testing cannot identify reactions to chemicals like food additives.