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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
In this study, we re-confirmed that none of the conventional ELISA buffers are capable of blocking the significantly high BG noise reaction caused by hydrophobic binding of immunoglobulin components in human sera to plastic surfaces (Fig. 1 and Fig. 2).