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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
Virtually all TSH assays currently in use. are either second or third generation, with functional sensitivities of 0.1 to 0.2 ÂµIU/mL. and 0.01 to 0.02 ÂµIU/mL, respectively; thus third generation assays are an order of. magnitude more sensitive than second generation assays (sTSH).