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What is the difference between a spectrophotometer and a photometer? A photometer simply measure the total lumens of a light source, while a spectrophotometer will measure a whole spectral of that same light source.
The emission filter further screens the light, the emitted light is measured by the detector, and the fluorescence value is displayed on the instrument. Figure 2 illustrates the key components of a filter fluorometer: light source/lamp; excitation and emission filters; sample cell/cuvette; and light detector.or example, if the substance is linear to 250 ppm and measurable with a calibration curve to 1000 ppm, you might calibrate with a 200 ppm standard and take readings at 500, 750, and 1000 ppm for a calibration curve. In some cases, calibration with a standard of known concentration is not necessary.