In E. coli the main enzyme is DNA polymerase III and the enzyme that replaces the RNA primer with DNA is DNA polymerase I. When the RNA primer has been replaced with DNA, there is a gap between the two Okazaki fragments and this is sealed by DNA ligase. â Back to Review.
Unsourced material may be challenged and removed. (October 2013).  Two Out of Three Ain't Bad  is a love song performed by the American musician Meat Loaf in his solo career, preceded by  You Took the Words Right Out of My Mouth  and followed by  Paradise by the Dashboard Light . It is a track off his 1977 album Bat Out of Hell, written by Jim Steinman.
Growing up in Detroit, Aretha Franklin and her sisters Carolyn and Erma sang in a choir directed by gospel great James Cleveland at the church pastored by their father, Rev. C.L. Franklin. Years later, their church-honed harmonies would be heard on hits issued on Atlantic Records during Aretha Franklin's mid-'60s heyday. While her pumpin' million-sellers such as Respect and Think may be better known, Ain't No Way, a tender gem written by Carolyn Franklin, is one of the singer's best sides. The low-key ballad has a sparse mix that highlights Aretha's aching lead vocal and the pensive tone of the lyrics.