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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
ELISA, or EIA, is an acronym for enzyme-linked immuno assay. ELISA is a test that detects and measures antibodies in your blood. This test can be used to determine if you have antibodies that are related to certain infectious conditions.Antibodies are proteins that the body produces in response to harmful substances (antigens).An ELISA test may be used to diagnose: 1  HIV (the virus that causes AIDS). 2  Lyme disease.3  pernicious anemia.ntibodies are proteins that the body produces in response to harmful substances (antigens). An ELISA test may be used to diagnose: 1  HIV (the virus that causes AIDS). 2  Lyme disease. 3  pernicious anemia.