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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
The most powerful ELISA assay format is the sandwich assay. This type of capture assay is called a âsandwichâ assay because the analyte to be measured is bound between two primary antibodies â the capture antibody and the detection antibody.The sandwich format is used because it is sensitive and robust.owever an antigen is captured to the plate (by direct adsorption to the surface or through a pre-coated capture antibody, as in a sandwich ELISA), it is the detection step (as either direct or indirect detection) that largely determines the sensitivity of an ELISA.