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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
The use of enzyme linked immunosorbent assay, or ELISA, provides an economical, rapid and highly sensitive method for screening a large number of samples. ELISA can be used to detect and quantitate peptides, proteins or antibodies.The assay is based upon an antigen-antibody interaction and subsequent enzymatic action on a substrate yielding a soluble colored product. Variations of the basic method exist for specialized applications. A basic method is outlined below.otes. It may be required to optimize the amount of a reagent in the assay by performing a checkerboard titration. This is accomplished by serial dilution of one reagent across the plate and serial dilution of the other reagent down the plate.