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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
The Enzyme-Linked Immunosorbent Assay (ELISA) is a technique used to detect antibodies or infectious agents in a sample.or an antigen ELISA, antibodies are bound to a plastic surface, a sample is added and if antigens from the virus we are testing for are present they will stick to the antibodies. This test then proceeds in the same way as the antibody ELISA. Figure 1. Detection of antigen-antibody binding (IFA and ELISA tests).