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Practical aspects of Ab assays Each Ab assay has its own characteristic advantages and disadvantages based on technical difficulty, intra- and interassay variability (i.e., precision), sensitivity for measuring Abs, specificity for the target protein or ligand, susceptibility to false-positive or false-negative results, and cost.
Introduction. Sandwich ELISA (enzyme-linked immunosorbent assay) involves attachment of a capture antibody to a microplate. Then, samples containing unknown amount of the target protein or analyte of interest are added and bind to the capture antibody.he ELISA method is a benchmark for quantitation of antigens. ELISAs are adaptable to high-throughput screening because results are rapid, consistent and relatively easy to analyze. The best results have been obtained with the sandwich format, utilizing highly purified, pre-matched capture and detection antibodies.