Unit 5: Hemoglobin Testing 26 Original: October 1999 Revision: September 2010 5 â 5 Recording Hemoglobin Values Objectives After completing this section, you will be able to: Correctly record hemoglobin values. Correctly record reasons why a hemoglobin value was not taken.
Group 7 â EDTA and ESR tubes. Black top ESR-Vacuum top with 3.2% sodium citrate: used for Westergren sedimentation rate. Group 9 â Yellow top with ACD Solution A. Yellow top tube with ACD (acid citrate dextrose) Solution A: used for whole blood determinations including flow cytometry and tissue typing assays.
SERUM, PLASMA OR WHOLE BLOOD COLLECTION. Draw blood in the color-coded VacutainerÂ® tube indicated in the alphabetical test listing. For serum or plasma, draw approximately 2 1/2 times the requested volume. For serum, allow the blood to clot sixty minutes and separate by centrifugation. For plasma and whole blood, completely fill the Vacutainer whenever possible to eliminate dilution from the anticoagulant or preservative and immediately mix the blood by gently and thoroughly inverting the tube five to ten times.