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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Polymerase Chain Reaction (PCR) The polymerase chain reaction (PCR) is a technique widely used in molecular biology for analyzing and testing DNA. The DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication contributed to the name for this technology. The PCR process sets in motion a chain reaction in which the DNA template is exponentially amplified (copied).