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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
The Polymerase Chain Reaction (PCR), is the technique used to detect small amounts of DNA and make enough copies of it to be detectable.All cells, bacteria and viruses contain DNA that is unique to them.he prepared sample is then added to a PCR mixture containing small pieces of DNA (primers) which match the DNA we are testing for, building blocks of DNA, and a polymerase enzyme which can join the blocks together to make copies of DNA.