Unit 5: Hemoglobin Testing 26 Original: October 1999 Revision: September 2010 5 â 5 Recording Hemoglobin Values Objectives After completing this section, you will be able to: Correctly record hemoglobin values. Correctly record reasons why a hemoglobin value was not taken.
Group 7 â EDTA and ESR tubes. Black top ESR-Vacuum top with 3.2% sodium citrate: used for Westergren sedimentation rate. Group 9 â Yellow top with ACD Solution A. Yellow top tube with ACD (acid citrate dextrose) Solution A: used for whole blood determinations including flow cytometry and tissue typing assays.
Blood collection tubes must be drawn in a specific order to avoid cross-contamination of additives between tubes. 1  The recommended order of draw for plastic vacutainer tubes is: 2  First - blood culture bottle or tube (yellow or yellow-black top) 3  Second - coagulation tube (light blue top). If just a routine coagulation assay is the only test ordered, then a single light blue top tube may be drawn. If there is a concern regarding contamination by tissue fluids or thromboplastins, then one may draw a non-additive tube first, and then the light blue top tube. Third - non-additive tube (red top) Last draw - additive tubes in this order: SST (red-gray or gold top). Contains a gel separator and clot activator.