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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Polymerase chain reaction (PCR) is a DNA based method of testing used to selectively target and replicate a specific region of DNA. Conventional PCR uses a forward and reverse primer containing nucleotides specifically arranged to compliment the target region of interest.