Sponsored Topics. Marlton is a census-designated place and unincorporated area located within Evesham Township in Burlington County, New Jersey. As of the United States 2000 Census, the population of Marlton was 10,260. Marlton was founded by Welsh and English farmers beginning in 1676.
ADVERTISEMENTS: Some of the major steps involved in polymerase chain reaction in DNA sequence are: 1. Step 1: Denaturation by Heat 2. Step 2: Annealing Primer to Target Sequence 3. Step 3: Extension 4. Step 4: End of the First PGR Cycle. The polymerase chain reaction (PGR) amplifies a single piece of DNA across several [â¦]
In the second step, the temperature is lowered and the two DNA strands become templates for DNA polymerase to selectively amplify the target DNA. The selectivity of PCR results from the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions. If the genomic DNA sequence of a gene is known, RT-PCR can be used to map the location of exons and introns in the gene. 2  The 5' end of a gene (corresponding to the transcription start site) is typically identified by RACE-PCR (Rapid Amplification of cDNA Ends).