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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
The polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication.As PCR progresses, the DNA thus generated is itself used as a template for replication.CR is a process that is done to take a little bit of DNA as well as the enzyme DNA polymerase and amplify (increase) the DNA sample. In short, it's a way to make more DNA from a small sample. Source(s): tigerbyte16 Â· 7 years ago.