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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Polymerase chain reaction (PCR) is a technique that is used to amplify trace amounts of DNA (and in some instances, RNA) located in or on almost any liquid or surface where DNA strands may be deposited.