Re: The Effect of Changing the Concentration of an Enzyme... by BioWizard on April 6th, 2010, 2:38 am. Obviously in this case, the increased concentration of enzyme results in an increased rate of reaction, up to a given point when all of the active sites of the enzyme are used up. That statement is phrased weird. The more enzyme you add, the more sites there is. Thus, as you add enzyme, you increase the concentration of available sites, not the other way around. I suppose what you meant to say is up to a given point where all the substrate molecules are bound to enzyme.
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