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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
In molecular biology, the polymerase chain reaction (PCR) is a technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. ^ In the 1980s, he invented the polymerase chain reaction (PCR), a central technique in molecular biology which allows the amplification of specified DNA sequences.