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The real-time fluorescence-based quantitative polymerase chain reaction (qPCR) has. become the benchmark technology for the detection of nucleic acids in every area of. microbiology, biomedical research, biotechnology and in forensic applications. Unlike. conventional (legacy) PCR, which is a qualitative end-point assay, qPCR allows accurate.
Polymerase Chain Reaction (PCR) is a molecular biological technique for amplifying (creating multiple copies of) DNA without using a living organ ism, such as E. coli or yeast.olymerase Chain Reaction (PCR). Polymerase chain reaction (PCR) is a laboratory technique used to amplify DNA sequence s. The method involves using short DNA sequence s called primer s to select the portion of the genome to be amplified. PCR (polymerase chain reaction).