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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
Different factors decide how long nicotine stays in the blood. In majority of smokers, nicotine and its by-product cotinine remain in the blood for about 3-4 days after quitting. This is naturally dependent on the frequency of smoking.