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TRH stimulation test. Prior to the availability of sensitive TSH assays, thyrotropin releasing hormone (TRH) stimulation tests were relied upon for confirming and assessing the degree of suppression in suspected hyperthyroidism. Typically, this stimulation test involves determining basal TSH levels and levels 15 to 30 minutes after an intravenous bolus of TRH.
The hypothalamus produces a hormone called thyrotropin-releasing hormone (TRH). TRH travels to the pituitary gland and stimulates it to release thyroid-stimulating hormone (TSH). TSH travels to the thyroid gland and stimulates it to release thyroid hormones, T3 and T4.