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Even if the substance is present in slightest amount in the bloodstream the test will detect its presence and indicate accordingly. Nicotine stays in your blood for maximum 3 days after the last nicotine product you consume.
When nicotine is absorbed into the body, it breaks down into cotinine. A blood test for nicotine will usually include a measurement of cotinine levels. Nicotine has a half-life of roughly two hours, meaning that half the nicotine in the body converts to cotinine every two hours. Cotinine's half-life is roughly twenty hours.