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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
Overview. Cortisol is a naturally occurring hormone that is secreted by the adrenal glands located on your kidneys. The levels of cortisol within your body fluctuate normally throughout the day and night.nfertility. Men with high cortisol levels can experience impotence, a medical condition characterized by the inability to have or maintain an erection. These symptoms of cortisol imbalance may also be accompanied by decreased sexual desire.