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TRH stimulation test. Prior to the availability of sensitive TSH assays, thyrotropin releasing hormone (TRH) stimulation tests were relied upon for confirming and assessing the degree of suppression in suspected hyperthyroidism. Typically, this stimulation test involves determining basal TSH levels and levels 15 to 30 minutes after an intravenous bolus of TRH.
Understanding the TRH Test. To understand how the TRH Stimulation Test works, it's first helpful to quickly review how the various brain hormones interact with the thyroid. Your hypothalamus is a gland in your brain that secretes thyrotropin-releasing hormone (TRH). When TRH is released, it stimulates your pituitary gland -- also in the brain -- to release thyroid stimulating hormone (TSH). TSH stimulates the thyroid itself to make thyroid hormones.