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A cortisol test measures the level of the hormone cortisol in a 24-hour sample of urine. The cortisol level may show problems with the adrenal glands or the pituitary gland. Cortisol is made by the adrenal glands.
Cortisol is a hormone in the adrenal cortex that surges in response to the release of ACTH, another hormone produced by the pituitary gland. Cortisol levels are a good indicator of adrenal and pituitary function. Elevated cortisol levels can be a sign of several medical disorders.