Enzymes have active sites where they come into contact with particular substrates. The catalytic properties of enzymes are a cyclic process. Once a substrate has come into contact with the active site of an enzyme, it is modified by the enzyme to form the end product.
1 Reducing saturated fat to no more than 5 to 6 percent of total calories. 2  For someone eating 2,000 calories a day thatâs about 11 to 13 grams of saturated fat. 3  Reducing the percent of calories from trans fat.
Type of fat â- Recommendation. Saturated Fats â Up to 10% of total daily calories. Dietary Cholesterol â Max 300 milligrams per day. USDA and HHS recommended that the intake of trans fat be as low as possible, although did not yet set the upper limit for trans fats.On the other hand, the American Heart Association has established an upper limit for trans fat-maximum 1% of total daily calories.ietary Cholesterol â Max 300 milligrams per day. USDA and HHS recommended that the intake of trans fat be as low as possible, although did not yet set the upper limit for trans fats. On the other hand, the American Heart Association has established an upper limit for trans fat-maximum 1% of total daily calories.