Edible anurans have to rely on more than just poison glands which tend to work too late (i.e. after the predator has it in his mouth!) Related articles: How to Rid Your Garden of Toads-tips for dog owners (aside from training the dog of course!)
Manevalâs staining method is a negative staining method. To stain a capsule of a cell by Manevalâs staining method. 1  1 % Congo red solution.  Manevalâs stain.
Procedure of Capsule Staining. 1  Place a small drop of a negative stain (India Ink, Congo Red, Nigrosin, or Eosin) on the slide. Congo Red is easier to see, but it does not work well with some strains. 2  Using sterile technique, add a loopful of bacterial culture to slide, smearing it in the dye.