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1. In some cases, the cofactor completes the active site of the enzyme or modifies it in such a manner that substrate binding can ensue. 2. The cofactor acts as a donor of electrons or atoms to the substrate and following the reaction is returned to its former state.
The flexibility of. that enzyme could be affected due to the effect of temperature on covalent and hydrogen bonds. Chemical bonds make up the shape of protein and proteinâs function is related to its shape and. therefore activity would decrease or impact the enzyme catalyzing a reaction (Reece, et al 2010).