Edible anurans have to rely on more than just poison glands which tend to work too late (i.e. after the predator has it in his mouth!) Related articles: How to Rid Your Garden of Toads-tips for dog owners (aside from training the dog of course!)
Manevalâs staining method is a negative staining method. To stain a capsule of a cell by Manevalâs staining method. 1  1 % Congo red solution.  Manevalâs stain.
The stains of the Capsule staining technique. Typically an acidic stain such as Congo red or nigrosin, which stains the background, and a basic stain that colorizes the cell proper are used.This technique begins as a negative stain; cells are spread in a film with an acidic stain and are not heat-fixed.