Place a few drops, or a small amount of suspension base nail polish on a paper plate. Donât be afraid to use a little portion of the nail polish to test. The glitter needs a little room anyway! Shake in a bit of the glitter combination you have already created into the few drops of polish.
CpG islands are defined as sequence ranges where the Obs/Exp value is greater than 0.6 and the GC content is greater than 50%. The expected number of CpG dimers in a window is calculated as the number of 'C's in the window multiplied by the number of 'G's in the window, divided by the window length.
Melting curve produced at the end of real-time PCR. A real-time polymerase chain reaction (RT-PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR.