In bulk protein purification, a common first step to isolate proteins is precipitation with ammonium sulphate (NH 4) 2 SO 4. This is performed by adding increasing amounts of ammonium sulphate and collecting the different fractions of precipitate protein.rotein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.
Phenolic disinfectants are effective bactericides, fungicides, tuberculocides and virucides, but are ineffective against spore-forming bacteria such as Clostridium difficile. EPA-registered phenolic disinfectants are used to disinfect surface areas and non-critical medical devices.
A large number of such chemical agents are in common use. Some of the more common groups are listed below: 1. Phenol and phenol derivatives Phenol (5-10%) was the first disinfectant commonly used. However, because of its toxicity and odor, phenol derivatives (phenolics) are now generally used.