In bulk protein purification, a common first step to isolate proteins is precipitation with ammonium sulphate (NH 4) 2 SO 4. This is performed by adding increasing amounts of ammonium sulphate and collecting the different fractions of precipitate protein.rotein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms.
Phenolic disinfectants are effective bactericides, fungicides, tuberculocides and virucides, but are ineffective against spore-forming bacteria such as Clostridium difficile. EPA-registered phenolic disinfectants are used to disinfect surface areas and non-critical medical devices.
Disinfection can be attained by means of physical or chemical disinfectants. The agents also remove organic contaminants from water, which serve as nutrients or shelters for microorganisms. Disinfectants should not only kill microorganisms. Disinfectants must also have a residual effect, which means that they remain active in the water after disinfection. A disinfectant should prevent pathogenic microorganisms from growing in the plumbing after disinfection, causing the water te be recontaminated.