Use DH5Î± cells in most cases. b. If want to cut at XbaI or other DAM-enzyme site, use SCS110 cells which are deficient in Dam and Dcm methylases. 2) Turn on water bath to 42 Î¿ C. 3) Put competent cells in a 1.5 ml tube (Eppendorf or similar). For transforming a DNA construct, use 50 ul of competent cells. For transforming a ligation, use 100 ul of competent cells. You may need more or less cells, depending how competent they are. 4) Keep tubes on ice.
Melinda Gates. Melinda Ann Gates (nÃ©e French; born August 15, 1964), DBE is an American philanthropist, former Microsoft employee, and wife of Microsoft founder Bill Gates. She is co-founder of the Bill & Melinda Gates Foundation. She worked at Microsoft, where she was project manager for Microsoft Bob, Encarta and Expedia.
William Henry Gates III, KBE is an American business magnate, investor, author, philanthropist, humanitarian, and co-founder of the Microsoft Corporation along with Paul Allen. In 1975, Gates and Allen launched Microsoft, which became the world's largest PC software company. During his career at Microsoft, Gates held the positions of chairman, CEO and chief software architect, while also being the largest individual shareholder until May 2014. Gates stepped down as chief executive officer of Mic