If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Ney Nature Center â The Ney Nature Center is a 446-acre Environmental Learning Center and Le Sueur County Park located on the bluffs of the Minnesota River near Henderson, Minnesota. The property is adjacent to the old East Henderson depot and village site, which has public access to the Minnesota River. It is open to the public for hiking, birdwatching, and X-C skiing. It also serves as a regional teaching station for Community Education and Ney Foundation programming.