If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Sweetser is a town in Franklin, Pleasant and Richland townships, Grant County, Indiana, United States. The population was 1229 at the 2010 census.irst mention of Sweetser in the Methodist Church conference minutes was in 1875. Local preachers mentioned were John Hubert, Allen Meek, Andrew Green and Joseph Leazenby. The first Methodist church was located on the northwest corner of Main Street and State Road 18.