Procedure: 1  Divide the slide into two sections with grease pencil. 2  2Place a small drop of distilled water on each area. 3  Emulsify one or two colonies of Staphylococcus on blood agar plate on each drop to make a smooth suspension. 4  The test suspension is treated with a drop of citrated plasma and mixed well with a needle. Do not put anything in the other drop that serves as control. The control suspension serves to rule out false positivity due to auto agglutination. Clumping of cocci within 5-10 seconds is taken as positive.
The IOM report did not specify requirements for water but made general fluid intake recommendations based on survey data of 91 ounces (that's 11-plus cups a day) for women and 125 ounces (15-plus cups a day) for men. Remember, these guidelines are for total fluid intake, including fluid from all food and beverages.
Of this, they suggest that 70-80% of the daily water intake should come from drinks, and the remaining 20-30% should come from food. This is supported by the NHS who recommends that women should drink eight 200ml glasses and men should drink 10 200ml glasses of fluid per day NHS Choices: Water and drinks, April 2013. .