If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Batchelor, Lousiana, is located in Pointe CoupÃ©e Parish, which is part of the Baton Rouge Metropolitan Statistical Area. The parish seat is New Roads, near which is the well-known Parlange Plantation, built in 1750, a classic example of the French Colonial Style of plantation house, home of the Tenant and Parlange families, whose descendants still live there.