Procedure: 1  Divide the slide into two sections with grease pencil. 2  2Place a small drop of distilled water on each area. 3  Emulsify one or two colonies of Staphylococcus on blood agar plate on each drop to make a smooth suspension. 4  The test suspension is treated with a drop of citrated plasma and mixed well with a needle. Do not put anything in the other drop that serves as control. The control suspension serves to rule out false positivity due to auto agglutination. Clumping of cocci within 5-10 seconds is taken as positive.
The IOM report did not specify requirements for water but made general fluid intake recommendations based on survey data of 91 ounces (that's 11-plus cups a day) for women and 125 ounces (15-plus cups a day) for men. Remember, these guidelines are for total fluid intake, including fluid from all food and beverages.
These numbers are derived from dietary survey data, so they are really a summary of how much people do consume, rather than how much they should consume. The range is quite broad: for men aged 51-70, a daily consumption of about 1.9 liters total is indicated, including the water present in all foods and beverages. For women aged 51-70, the median consumption is about 2.0 liters per day from all sources.