If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Sylmar (in Los Angeles County, CA) Populated Place Profile. Sylmar (GNIS FID: 1661539) is a populated place LOCATED WITHIN the City of Los Angeles. In turn, the City of Los Angeles is located within Los Angeles County. Sylmar is located at latitude 34.308 and longitude -118.449. The elevation of Sylmar is 1,253 feet above mean sea level.