If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Sylmar, Los Angeles. Sylmar is a neighborhood in the San Fernando Valley region of Los Angeles, California. Historically, known for its profusion of olive orchards, Sylmar can trace its past to the 18th century and the founding of the San Fernando Mission. In 1890, olive production was begun in a systematic manner.