If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Granger, IA. Sponsored Topics. Granger is a city in Dallas and Polk counties in the U.S. state of Iowa. The population was 583 at the 2000 census. It is part of the Des MoinesâWest Des Moines Metropolitan Statistical Area. Granger is located at 41Â°45â²42â³N 93Â°49â²26â³W / 41.76167Â°N 93.82389Â°W / 41.76167; -93.82389 (41.761672, -93.823921).