Procedure: 1  Divide the slide into two sections with grease pencil. 2  2Place a small drop of distilled water on each area. 3  Emulsify one or two colonies of Staphylococcus on blood agar plate on each drop to make a smooth suspension. 4  The test suspension is treated with a drop of citrated plasma and mixed well with a needle. Do not put anything in the other drop that serves as control. The control suspension serves to rule out false positivity due to auto agglutination. Clumping of cocci within 5-10 seconds is taken as positive.
The IOM report did not specify requirements for water but made general fluid intake recommendations based on survey data of 91 ounces (that's 11-plus cups a day) for women and 125 ounces (15-plus cups a day) for men. Remember, these guidelines are for total fluid intake, including fluid from all food and beverages.
The Institute of Medicine lays out the approximate amount of water traditionally thought necessary for males and females of varying age groups and lifestyles in liters per day. The IOM recommends males 19 to over 70 years of age consume 3.7 liters of water daily. Women in the same age group only require 2.7 liters.