If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Fallout: New Vegas. Where is Ambassador Crocker? I dont know if its a glitch or not but when i walk into the NCR embassy crocker isnt in his office or anywhere in the building. I have the note from the NCR trooper asking me to meet with him, but he's nowhere to be found. Ive waited days and went back inside, but he's still not there. Is it possible that he's in McCarren, or Hoover Dam or something?