If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Pillager is a city in Cass County, Minnesota, United States. The population was 469 at the 2010 census. It is part of the Brainerd Micropolitan Statistical Area. Pillager is located in Sylvan Township (T133N R30W), on the left (North) bank of the Crow Wing River, just East of Lake Placid. According to the United States Census Bureau, it has a total area of 0.7 square miles (1.7 kmÂ²), of which, 0.6 square miles (1.7 kmÂ²) of it is land and 0.04 square miles (0.1 kmÂ²) of it (4.48%) is water.