Procedure: 1  Divide the slide into two sections with grease pencil. 2  2Place a small drop of distilled water on each area. 3  Emulsify one or two colonies of Staphylococcus on blood agar plate on each drop to make a smooth suspension. 4  The test suspension is treated with a drop of citrated plasma and mixed well with a needle. Do not put anything in the other drop that serves as control. The control suspension serves to rule out false positivity due to auto agglutination. Clumping of cocci within 5-10 seconds is taken as positive.
The IOM report did not specify requirements for water but made general fluid intake recommendations based on survey data of 91 ounces (that's 11-plus cups a day) for women and 125 ounces (15-plus cups a day) for men. Remember, these guidelines are for total fluid intake, including fluid from all food and beverages.
0.034 ounces x Daily Caloric Intake = Daily Fluid Requirement in ounces. As you calculate out what your daily fluid requirements are, you'll probably realize that it is close to that common recommendation of 8 - 12 cups per day. However, the above equations will give you a more accurate guideline because fluid needs vary depending on body size.