If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Brainerd is the principal city of the Brainerd Micropolitan Area, a micropolitan area covering Cass and Crow Wing counties and with a combined population of 91,067 as of the 2010 census. The Brainerd area serves as a major tourist destination for Minnesota, and Baxter is a regional retail center. Brainerd is the home to one of five medevac helicopter flight stations in the state for AirCare, operated by North Memorial Medical Center in Robbinsdale, a Level 1 Trauma Center.