If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Spicer is the son of Kathryn (nÃ©e Grossman) and Michael William Spicer (1944â2016). The Spicers were living in Port Washington when Sean was born at North Shore Hospital in Manhasset, New York. Spicer grew up in the East Bay area of Rhode Island.