If using PVDF, methanol can be removed from the transfer buffer altogether, and is only needed to activate the PVDF before assembling the gel/membrane sandwich. Choose wet transfer overnight at 4Â°C instead of semi-dry transfer.
Spicer is located on the shores of Green Lake, in the heart of the Little Crow Lakes Region, 95 miles west of Minneapolis / St. Paul. From the Metro area, take I-94 to Hwy 23 S. at the exit near St. Cloud. Continue south on Hwy. 23 to Spicer. Click on the Spicer area map at left for a larger view.
Browerville was named after Jacob V. Brower (1844â1905), who was a prolific writer of the Upper Midwest region of the United States who championed the location and protection of the utmost headwaters of the Mississippi and Missouri rivers. Brower was born in Michigan and moved to Minnesota. In 1862 he served with Henry Hastings Sibley during wars against the Sioux in Minnesota. After the war he was County Auditor and County Attorney for Todd County, Minnesota. According to the United States Census Bureau, the city has a total area of 69;square miles (1.8 kmÂ²), all of it land.