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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
Best Answer: In order for the DNA strands to separate,3 types of enzymes are needed. Helicases, single-strand binding proteins and topoisomerase. Priming: Once the replication fork is established a short segment of RNA, formed by an enzyme called primase, initiates the process of replication. With the primer in place, DNA polymerase catalyzes the synthesis of a new strand of DNA.