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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
DNA Replication. Â­DNA carries the information for making all of the cell's proteins. These proÂ­teins implement all of the functions of a living organism and determine the organism'Â­s characteristics. When the cell reproduces, it has to pass all of this information on to the daughter cells.owever, DNA does not unzip entirely. It unzips in a small area called a replication fork, which then moves down the entire length of the molecule. Let's look at the details: 1  An enzyme called DNA gyrase makes a nick in the double helix and each side separates. 2  An enzyme called helicase unwinds the double-stranded DNA.