The MacBook Pro follows, with its prices going from $2,199 down to $1,999 for the 15-inch version (and from $1,499 for the 13-inch version down to $1,299). That's a break from traditional Apple pricing, where prices would remain the same generation over generation, with updated components adding value.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.
The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Miniprimer PCR: uses a thermostable polymerase (S-Tbr) that can extend from short primers (smalligos) as short as 9 or 10 nucleotides. 2  This method permits PCR targeting to smaller primer binding regions, and is used to amplify conserved DNA sequences, such as the 16S (or eukaryotic 18S) rRNA gene.