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ATP synthase is a ubiquitous enzyme that is located in the inner membranes of mitochondria, thylakoid membranes of chloroplasts, or the plasma membranes of bacteria.
In plants, ATP synthase is also present in chloroplasts (CF 1 F O-ATP synthase). The enzyme is integrated into thylakoid membrane; the CF 1 -part sticks into stroma, where dark reactions of photosynthesis (also called the light-independent reactions or the Calvin cycle) and ATP synthesis take place.he same process takes place in the mitochondria, where ATP synthase is located in the inner mitochondrial membrane (so that F 1 -part sticks into mitochondrial matrix, where ATP synthesis takes place).