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ATP synthase is a ubiquitous enzyme that is located in the inner membranes of mitochondria, thylakoid membranes of chloroplasts, or the plasma membranes of bacteria.
ADP + Pi â ATP + Energy is consumed. So, the key difference between ATPase and ATP Synthase is, ATPase is the enzyme that breaks down ATP molecules while the ATP Synthase involves in ATP production. CONTENTS. 1. Overview and Key Difference 2. What is ATPase 3. What is ATP Synthase 4. Similarities Between ATPase and ATP Synthase 5.