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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
Before new daughter strands can be added on, the parent strands are first made ready by an RNA primer. The primer is built by the enzyme RNA primase. So, let's go to the chart: RNA primase is the enzyme that builds an RNA primer on the parent strand to initiate DNA replication.nce the RNA primer is built, then the next enzyme, DNA polymerase, is free to do its job. DNA polymerase slides into the replication fork and positions itself behind the RNA primer. It begins to add DNA nucleotides onto each parent strand.