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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
During replication, this can remove primers ahead of the polymerizing function of the polymerase. See Figure 3 . Figure 3. Another intimate function of DNA polymerase I (and of the other forms of DNA polymerase found in E. coli) is the 3â² to 5â² exonuclease activity. This activity can deâpolymerize DNA starting from the newly synthesized end. Imagining why DNA polymerase would have an activity that opposes the action of the enzyme is a little difficult. The 3â² to 5â² exonuclease activity serves an editing function to ensure the fidelity of replication. Suppose DNA polymerase were to make a mistake and add a T opposite a G in the template strand. When the enzyme begins the next step of polymerization, the T is not properly paired with the template.