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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
Key Concepts: Bacterial DNA polymerase I enzymes are multidomain proteins with separate polymerisation, proofreading and 5â²â3â² exonuclease functions. They perform a vital function in bacteria by carrying out DNA repair and some aspects of chromosomal replication.Polymerases incorporate one nucleotide at a time to the 3â² end of the primer DNA strand in a templateâdirected manner.hey perform a vital function in bacteria by carrying out DNA repair and some aspects of chromosomal replication. Polymerases incorporate one nucleotide at a time to the 3â² end of the primer DNA strand in a templateâdirected manner.