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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.eplication machineries include primosomes also. The factors are replication enzymes; DNA polymerase, DNA helicases, DNA clamps and DNA topoisomerases, and replication proteins; e.g. single-stranded DNA binding proteins (SSB). In the replication machineries these components coordinate.