The MacBook Pro follows, with its prices going from $2,199 down to $1,999 for the 15-inch version (and from $1,499 for the 13-inch version down to $1,299). That's a break from traditional Apple pricing, where prices would remain the same generation over generation, with updated components adding value.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.
Restriction Enzymes Cut DNA Molecules at Specific Sequences. To clone specific DNA fragments in a plasmid vector, as just described, or in other vectors discussed in later sections, the fragments must be produced and then inserted into the vector DNA.As noted in the introduction, restriction enzymes and DNA ligases are utilized to produce such recombinant DNA molecules.hen such a vector is treated with a restriction enzyme that recognizes a recognition sequence in the polylinker, it is cut at that sequence, generating sticky ends. In the presence of DNA ligase, DNA fragments produced with the same restriction enzyme will be inserted into the plasmid (Figure 7-8b).