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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
In addition to these proteins, several other enzymes are involved in bacterial DNA replication. 1  DNA Polymerase-DNA Polymerase I (Pol I) was the first enzyme discovered with polymerase activity, and it is the best characterized enzyme. 2  5' to 3' elongation (polymerase activity). 3  3' to 5' exonuclease (proof-reading activity). 4  5' to 3' exonuclease ....n addition to these proteins, several other enzymes are involved in bacterial DNA replication. DNA Polymerase-DNA Polymerase I (Pol I) was the first enzyme discovered with polymerase activity, and it is the best characterized enzyme.