The MacBook Pro follows, with its prices going from $2,199 down to $1,999 for the 15-inch version (and from $1,499 for the 13-inch version down to $1,299). That's a break from traditional Apple pricing, where prices would remain the same generation over generation, with updated components adding value.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.
A restriction map is a description of restriction endonuclease cleavage sites within a piece of DNA. Generating such a map is usually the first step in characterizing an unknown DNA, and a prerequisite to manipulating it for other purposes.Typically, restriction enzymes that cleave DNA infrequently (e.g. those with 6 bp recognition sites) and are relatively inexpensive are used to produce at a map.The DNA to be restriction mapped it usually contained within a well-characterized plasmid or bacteriophage vector for which the sequence is known.In fact, there are usually multiple known restriction sites immediately flanking the uncharacterized DNA, which facilitates making the map.he most straightforward method for restriction mapping is to digest samples of the plasmid with a set of individual enzymes, and with pairs of those enzymes.