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Compare RNA-dependent RNA polymerase. RNA polymerase (RNAP or RNApol), also known as DNA-dependent RNA polymerase, is an enzyme that produces primary transcript RNA. In cells, RNAP is necessary for constructing RNA chains using DNA genes as templates, a process called transcription.
The transcription is performed by an enzyme called RNA polymerase. To make mRNA, RNA polymerase: 1  Binds to the DNA strand at a specific sequence of the gene called a promoter. 2  Unwinds and unlinks the two strands of DNA.  Uses one of the DNA strands as a guide or template.