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Compare RNA-dependent RNA polymerase. RNA polymerase (RNAP or RNApol), also known as DNA-dependent RNA polymerase, is an enzyme that produces primary transcript RNA. In cells, RNAP is necessary for constructing RNA chains using DNA genes as templates, a process called transcription.
Synthesis [edit]. Although there are several methods for doing so, cDNA is most often synthesized from mature (fully spliced) mRNA using the enzyme reverse transcriptase. A eukaryotic cell transcribes the DNA (from genes) into RNA (pre-mRNA). 2  The same cell processes the pre-mRNA strands by removing introns, and adding a poly-A tail and 5â Methyl-Guanine cap (this is known as post-transcriptional modification). 3  This mixture of mature mRNA strands is extracted from the cell.