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Compare RNA-dependent RNA polymerase. RNA polymerase (RNAP or RNApol), also known as DNA-dependent RNA polymerase, is an enzyme that produces primary transcript RNA. In cells, RNAP is necessary for constructing RNA chains using DNA genes as templates, a process called transcription.
The starting point for transcription, where RNA polymerase unwinds the DNA and begins to make a complementary mRNA copy, is the promoter site; the region of DNA that is the endpoint of transcription is the terminator site; mRNA is synthesized in the 5â to 3â direction.uring transcription, the enzyme RNA polymerase synthesizes a strand of messenger RNA (mRNA) using one strand of the gene as a template. mRNA is synthesized using nucleotides that contain the bases A, C, G, and U (instead of T), which pair with the complementary bases of the DNA template strand.