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Compare RNA-dependent RNA polymerase. RNA polymerase (RNAP or RNApol), also known as DNA-dependent RNA polymerase, is an enzyme that produces primary transcript RNA. In cells, RNAP is necessary for constructing RNA chains using DNA genes as templates, a process called transcription.
First of all, since RNA polymerase synthesizes mRNA from 5' to 3' (just like DNA polymerase) the DNA template strand has to be read from 3' to 5' (just like when DNA polymerase uses a DNA template strand to synthesize a new complementary DNA strand).he starting point for transcription, where RNA polymerase unwinds the DNA and begins to make a complementary mRNA copy, is the promoter site; the region of DNA that is the endpoint of transcription is the terminator site; mRNA is synthesized in the 5â to 3â direction.