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Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.
Chandran 23 Dec 2014. The melting temperature of DNA is called as Tm. The Tm is defined as the temperature in degrees Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands.NA melting temperature is the temperature in degree Celsius, at which 50% of all molecules of a given DNA sequence are hybridized into a double strand, and 50% are present as single strands. 0 | 0.