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Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.
RE: Melting temperature and Annealing temp of PCR? Hi...I know that melting temp is the temp of primers at which 50% of the duplex will be single stranded..but what does it with annealing temp?Why we always have to keep lower annealing temp with respect to melting temp?nnealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.