Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Tax-free holiday in Missouri this weekend. This is an archived article and the information in the article may be outdated. Please look at the time stamp on the story to see when it was last updated. (KTVI) â The Missouri Sales-Tax Holiday is this weekend, August 1-3. That means sales tax on certain back-to-school products will be waived. Eligible items include clothing, footwear, computers and traditional school supplies. The tax waiver includes online orders as well.