Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Missouri retailers readying for sales tax holiday weekend. FILE PHOTO: More than 1000 shoppers were ready as the doors opened at Taubman Prestige Outlets in Chesterfield on Friday, Aug. 2, 2013. Missouriâs three-day sales tax holiday on clothing, school supplies and computers is this weekend, and retailers are readying for a rush of shoppers. The sales tax holiday temporarily exempts the stateâs portion of the tax rate â 4.225 percent â from Aug. 5-7 on certain back-to-school items.