Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Add to my trip. Missouri's annual back to school state tax-free weekend happens every year on the first full weekend in August. During this three-day period, you can purchase school supplies, clothing and computers without paying the state portion of the sales tax, which is typically 4.225%. This year, the tax holiday is Aug. 7-9, 2015.