Remember, your problem statement is the backbone of the proposal and the feasibility report. By giving careful consideration to how you construct it now (for the proposal), you can use it when doing your research and writing for the proposal as well as the progress and the feasibility report.
http://www.ohsu.edu/research/skachlab/an... follow this link and it will take to a page where it describe very clearly the sequences of event that occurs during translocation. animated clips are also included which i believed will allow you to understand the process more clearly.
In this case, a substance is chemically altered during its transport across a membrane so that once inside, the cytoplasmic membrane becomes impermeable to that substance and it remains within the cell. An example of group translocation in bacteria is the phosphotransferase system.