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Answer. It must occur in the nucleus where the DNA in the cell is located. However, once mRNA is produced, it leaves the nucleus and protein synthesis â translation â occurs in the cytoplasm.t must occur in the nucleus where the DNA in the cell is located. However, once mRNA is produced, it leaves the nucleus and protein synthesis â translation â occurs in the cytoplasm.
1 When RNA polymerase transcribes the DNA into a mRNA molecule, adenine pairs with uracil and cytosine pairs with guanine (A-U and C-G). 2  At the end of transcription, mRNA is transported to the cytoplasm for the completion of protein synthesis. Transfer RNA (tRNA) plays an important role in the translation portion of protein synthesis. 2  Its job is to translate the message within the nucleotide sequences of mRNA into specific amino acid sequences. 3  The amino acid sequences are joined together to form a protein.