Tampons shouldn't be worn overnight because... 1  Tampons need to be changed every 4-6 hours and many people sleep longer than this - changing often is important as tampons allow bacterial growth while preventing vaginal cleaning so the longer a tampon is worn the more bacteria can multiply within the vagina.
MacConkey agar is a selective medium containing bile salts and crystal violet that inhibits the growth of Gram-positive bacteria while allowing for the growth of Gram-negative bacteria.MacConkey agar is often used when investigating and culturing fecal bacteria.he MacConkey agar we talked about as an example of a selective media is also a differential media. In addition to the selective bile salts and crystal violet, MacConkey agar contains a pH indicator and the sugar lactose.
Microbiology Theory: Media Preparation. Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for their needs, in the laboratory, however, these requirements must be met by a culture medium.