Use an annealing time of at least 30 sec. Annealing temperature was too high. If the annealing temperature is too high, primers are unable to bind to the template. The rule of thumb is to use an annealing temperature that is 5Â°C lower than the T m of the primer.
(KTVI)- The Missouri annual sales tax holiday is August 7th- 9th. The list of tax free items includes clothing, computers and school supplies. The state sales tax will not be charged on the following items during the holiday: Clothing that does not have a value of more than $100.
Missouri Tax-Free Weekend Brings Shoppers to Branson. From August 5th to August 7th, the State of Missouri is holding its now-annual Back to School Sales Tax Holiday. This means there will be no state sales tax on items that could be used for back-to-school, such as clothing and computers.