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Primer sequences are determined from known sequence since there must be a match to the region of DNA to be amplified. PCR amplification requires 2 primers that determine the region of sequence amplified in the forward and reverse direction.ikewise, the reverse primer is designed from the complimentary strand. PCR is exponential amplification in which the newly generated PCR fragment from one cycle also acts as a template for the next cycle (Figure 1A). Amplification of DNA for Sanger sequencing differs from PCR in that a single primer is used.
Transistor as Amplifier. What is Amplifier: Amplification is a process of increasing the signal strength by increasing the amplitude of a given signal with out. changing its characteristics. The input signal may be a current signal, voltage signal or a power signal; amplifier will. amplify the signal without changing its characteristics.