At the end of the column they elute one at a time. During the entire chromatography process the eluent is collected in a series of fractions. Fractions can be collected automatically by means of fraction collectors. The productivity of chromatography can be increased by running several columns at a time.
dash - the act of moving with great haste; he made a dash for the door bolt rush, rushing, haste, hurry - the act of moving hurriedly and in a careless manner; in his haste to leave he forgot his book
In other words, underlining something is equivalent to italicizing it, so it is not proper to both italicize and underline a title. (And, as with italics and quotation marks, titles are not underlined at the head of an essay or article.)