1 Yellow media without gas pocket-sugar utilization with production of acid only. 2  Red media (no change in media) -no change and no sugar utilization. 3  Pink media especially at the top, ignore gas-no sugar utilization with degradation of protein resulting in alkaline end products (this may be difficult to see).
With organic solvents with long alkane chain, such as n-hexadecane and n-decane, the water droplets become much larger and the microemulsion is unstable. In this case, solvent polarity may have become a determining factor and the long alkane chain was too hydrophobic to form a stable microemulsion with water.
If benzene is forced to react by increasing the temperature and/or by addition of a catalyst, It undergoes substitution reactions rather than the addition reactions that are typical of alkenes. This further confirms the previous indication that the six-carbon benzene core is unusually stable to chemical modification. The conceptual contradiction presented by a high degree of unsaturation (low H:C ratio) and high chemical stability for benzene and related compounds remained an unsolved puzzle for many years.