In the meantime, here's a sneak peek, just a small sliceâthe first part of a 2-part excerpt from the addendum of Grantsmanship: Program Planning & Proposal Writing. The topic is the difference between a planning and an implementation (or program) grant proposal. Enjoy!
What is PCR (polymerase chain reaction)? Polymerase chain reaction (PCR) is a technique that is used to amplify trace amounts of DNA (and in some instances, RNA) located in or on almost any liquid or surface where DNA strands may be deposited.
In the late 1990s, nested PCR was used to the detect the virus as it showed improved sensitivity over non-nested PCR. Now, quantitative PCR assays offered as-good or better sensitivity than nested PCR, fast turnaround time in the lab, and lower rates of cross-contamination via closed-tube amplification.