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12 weeks of age: Administer the third combination injection and possibly a lyme vaccine inoculation. Generally a lyme vaccine is repeated two weeks later, then once a year. 16 weeks of age: Adminster the last combination vaccine. 12 to 16 weeks of age: Adminster rabies vaccine. The timing of this vaccine may depend on the laws in your area (since this can be a human disease, too). Confirm with your veterinarian and check your local and state laws.
Generally, veterinarians recommend that puppies receive their second combination vaccine shot at around nine weeks old. The third combination injection should come when they are about 12 weeks old. Sometimes a Lyme's disease vaccine is recommended at this time as well.