Coenzyme A (CoA, CoASH, or HSCoA) is a coenzyme, notable for its role in the synthesis and oxidation of fatty acids, and the oxidation of pyruvate in the citric acid cycle. All genomes sequenced to date encode enzymes that use coenzyme A as a substrate, and around 4% of cellular enzymes use it (or a thioester, such as acetyl-CoA) as a substrate. In humans, CoA biosynthesis requires cysteine, pantothenate, and adenosine triphosphate (ATP).[1]
TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat.#R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.he enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.
Such is the history of Taq DNA polymerase. The original report of this enzyme, purified from the hot springs bacterium Thermus aquaticus, was published in 1976. Roughly 10 years later, the polymerase chain reaction was developed and shortly thereafter Taq became a household word in molecular biology circles.Currently, the world market for Taq polymerase is in the hundreds of millions of dollars each year.oughly 10 years later, the polymerase chain reaction was developed and shortly thereafter Taq became a household word in molecular biology circles. Currently, the world market for Taq polymerase is in the hundreds of millions of dollars each year.