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12 weeks of age: Administer the third combination injection and possibly a lyme vaccine inoculation. Generally a lyme vaccine is repeated two weeks later, then once a year. 16 weeks of age: Adminster the last combination vaccine. 12 to 16 weeks of age: Adminster rabies vaccine. The timing of this vaccine may depend on the laws in your area (since this can be a human disease, too). Confirm with your veterinarian and check your local and state laws.
Rabies Vaccinations for Puppies. Vaccinations for puppies are especially important as puppies are vulnerable to a much wider range of diseases than are adult dogs. Puppy vaccination regimens usually begin at between six and four weeks of age.