In the meantime, here's a sneak peek, just a small sliceâthe first part of a 2-part excerpt from the addendum of Grantsmanship: Program Planning & Proposal Writing. The topic is the difference between a planning and an implementation (or program) grant proposal. Enjoy!
What is PCR (polymerase chain reaction)? Polymerase chain reaction (PCR) is a technique that is used to amplify trace amounts of DNA (and in some instances, RNA) located in or on almost any liquid or surface where DNA strands may be deposited.
The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Miniprimer PCR: uses a thermostable polymerase (S-Tbr) that can extend from short primers (smalligos) as short as 9 or 10 nucleotides. 2  This method permits PCR targeting to smaller primer binding regions, and is used to amplify conserved DNA sequences, such as the 16S (or eukaryotic 18S) rRNA gene.