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12 weeks of age: Administer the third combination injection and possibly a lyme vaccine inoculation. Generally a lyme vaccine is repeated two weeks later, then once a year. 16 weeks of age: Adminster the last combination vaccine. 12 to 16 weeks of age: Adminster rabies vaccine. The timing of this vaccine may depend on the laws in your area (since this can be a human disease, too). Confirm with your veterinarian and check your local and state laws.
Having your Puppy Vaccinated. Each state has its own requirements regarding the scheduling of rabies vaccinations, but most puppies should receive their first dose of the rabies vaccine between 12 to 16 weeks of age. This is about the time her mother's immunity wears off, and she becomes vulnerable to the virus.