Coenzyme A (CoA, CoASH, or HSCoA) is a coenzyme, notable for its role in the synthesis and oxidation of fatty acids, and the oxidation of pyruvate in the citric acid cycle. All genomes sequenced to date encode enzymes that use coenzyme A as a substrate, and around 4% of cellular enzymes use it (or a thioester, such as acetyl-CoA) as a substrate. In humans, CoA biosynthesis requires cysteine, pantothenate, and adenosine triphosphate (ATP).[1]
TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat.#R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.he enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.
Therefore it replaced the DNA polymerase from E. coli originally used in PCR [7]. Taq's temperature optimum for activity is 75-80 0 C, with a half-life of 9 minutes at 97.5 0 C, and can replicate a 10 3 base pair strand of DNA in less than 10 seconds at 72 0 C [8].The incorporation of dNTPs into DNA is catalyzed by Taq DNA polymerase I.aq DNA polymerase has among the highest error rates of the thermophilic polymerases at 285 x 10 -6 errors per template nucleotide. Tli polymerase has a proof reading ability that is five-fold better than Taq at 57 x 10 -6 errors per template nucleotide and Pfu polymerase also demonstrates fidelity in this range [10].