1 Yellow media without gas pocket-sugar utilization with production of acid only. 2  Red media (no change in media) -no change and no sugar utilization. 3  Pink media especially at the top, ignore gas-no sugar utilization with degradation of protein resulting in alkaline end products (this may be difficult to see).
With organic solvents with long alkane chain, such as n-hexadecane and n-decane, the water droplets become much larger and the microemulsion is unstable. In this case, solvent polarity may have become a determining factor and the long alkane chain was too hydrophobic to form a stable microemulsion with water.
Myoglobin can bind a single oxygen molecule between the distal histidine and the heme iron atom. Hydrogen bonding of the distal histidine and the oxygen crowds the area enough to cause the oxygen to bind at an angle with respect to the plane of the heme group (Pratt and Cornely 2004).