1 Yellow media without gas pocket-sugar utilization with production of acid only. 2  Red media (no change in media) -no change and no sugar utilization. 3  Pink media especially at the top, ignore gas-no sugar utilization with degradation of protein resulting in alkaline end products (this may be difficult to see).
With organic solvents with long alkane chain, such as n-hexadecane and n-decane, the water droplets become much larger and the microemulsion is unstable. In this case, solvent polarity may have become a determining factor and the long alkane chain was too hydrophobic to form a stable microemulsion with water.
When the double bond is substituted with another alkyl group, the electrons in the carbon-hydrogen bond of that alkyl group can stablilze the vacant anit-bonding Pi orbital of the double bond. This is call hyperconjugation.This leads to a net positive interaction, which makes the double bond more stable.ubstituted alkenes are more physically stable. The fact that the activation energy for a tri subst. alkene is lower is because any carbocation intermediate is more stable with increasing substitution. See the wikipedia article here: http://en.wikipedia.org/wiki/Hyperconjug...