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The Prokaryotic Cell. Prokaryotes are unicellular organisms that lack organelles or other internal membrane-bound structures . Therefore, they do not have a nucleus, but, instead, generally have a single chromosome: a piece of circular, double-stranded DNA located in an area of the cell called the nucleoid. Most prokaryotes have a cell wall outside the plasma membrane.
All prokaryotic cells contain large quantities of genetic material in the form of DNA and RNA. Because prokaryotic cells, by definition, do not have a nucleus, the single large circular strand of DNA containing most of the genes needed for cell growth, survival, and reproduction is found in the cytoplasm.rokaryotes known as  gram-negative bacteria , for example, often have two plasma membranes with a space between them known as the periplasm. As in all cells, the plasma membrane in prokaryotic cells is responsible for controlling what gets into and out of the cell.