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The Prokaryotic Cell. Prokaryotes are unicellular organisms that lack organelles or other internal membrane-bound structures . Therefore, they do not have a nucleus, but, instead, generally have a single chromosome: a piece of circular, double-stranded DNA located in an area of the cell called the nucleoid. Most prokaryotes have a cell wall outside the plasma membrane.
â¢ In eukaryotes, multiple replication sites are present in a single DNA molecule whereas, in prokaryotes, a single replication site is present in the circular DNA molecule. â¢ In prokaryotes, DNA replication involves three polymerase enzymes; namely, DNA polymerase I, DNA polymerase II, and DNA polymerase III.rokaryotic DNA and eukaryotic DNA vary widely; so do their replication processes. Prokaryotic DNA Replication. Unlike in eukaryotes, there is a single circular DNA exists in prokaryotes. The replication in the prokaryotic chromosome begins at the origin of replication.