1 Yellow media without gas pocket-sugar utilization with production of acid only. 2  Red media (no change in media) -no change and no sugar utilization. 3  Pink media especially at the top, ignore gas-no sugar utilization with degradation of protein resulting in alkaline end products (this may be difficult to see).
With organic solvents with long alkane chain, such as n-hexadecane and n-decane, the water droplets become much larger and the microemulsion is unstable. In this case, solvent polarity may have become a determining factor and the long alkane chain was too hydrophobic to form a stable microemulsion with water.
Thus neither hexane nor iodine dissolves in water. Polar and ionic solutes do not dissolve in non-polar solvents because they have a stronger attraction for each other than for the non-polar solvent molecules.Thus neither water nor potassium permanganate dissolves in hexane. Iodine is a non-polar molecule because the iodine-iodine bond is pure covalent.Hexane is non-polar because the electronegativity difference between the hydrogen and carbon atoms is very small. Water is polar because the electronegativity difference between the hydrogen and oxygen atoms is large.hus neither hexane nor iodine dissolves in water. Polar and ionic solutes do not dissolve in non-polar solvents because they have a stronger attraction for each other than for the non-polar solvent molecules.