Coenzyme A (CoA, CoASH, or HSCoA) is a coenzyme, notable for its role in the synthesis and oxidation of fatty acids, and the oxidation of pyruvate in the citric acid cycle. All genomes sequenced to date encode enzymes that use coenzyme A as a substrate, and around 4% of cellular enzymes use it (or a thioester, such as acetyl-CoA) as a substrate. In humans, CoA biosynthesis requires cysteine, pantothenate, and adenosine triphosphate (ATP).[1]
TaKaRa Taq DNA Polymerase is a versatile and thermostable polymerase suitable for a variety of standard PCR applications. The enzyme is also supplied as a Premix Taq DNA polymerase (Cat.#R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.This premix formulation simplifies PCR setup and minimizes pipetting steps. This is particularly helpful for high-throughput screening projects.he enzyme is also supplied as a Premix Taq DNA polymerase (Cat. #R004A), a 2X PCR master mix containing Taq enzyme, buffer and dNTPs.
In the presence of Mn 2+ , Tfl DNA polymerase can use RNA as a template. Tfl DNA polymerase exhibits a 5â²â3â² exonuclease activity but lacks a 3â²â5â² exonuclease activity. This enzyme is commonly used in PCR (Gaensslen et al. 1992), where its activity is similar to that of Taq DNA polymerase.Tfl DNA polymerase is used in the Access and AccessQuickâ¢ RT-PCR Systems.aq DNA polymerase is suitable for most PCR applications that do not require a high-fidelity enzyme, such as detecting specific DNA or RNA sequences. The error rate of Taq DNA polymerase is approximately 1 Ã 10 â5 errors/base, although the fidelity does depend somewhat on the reaction conditions.