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A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.It is mainly associated with retroviruses.elomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
â¢ REVERSE TRANSCRIPTASE (noun). The noun REVERSE TRANSCRIPTASE has 1 sense: 1. a polymerase that catalyzes the formation of DNA using RNA as a template; found especially in retroviruses. Familiarity information: REVERSE TRANSCRIPTASE used as a noun is very rare. REVERSE TRANSCRIPTASE (noun). The noun REVERSE TRANSCRIPTASE has 1 sense: 1. a polymerase that catalyzes the formation of DNA using RNA as a template; found especially in retroviruses. Familiarity information: REVERSE TRANSCRIPTASE used as a noun is very rare.