If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Missouri Sales Tax Holiday 2016. By Missouri state law, the sales tax holiday begins on the first Friday in August and continues through the following Sunday. In 2016, the three-day holiday begins at 12:01 a.m. on Friday, Aug. 5, and runs through Sunday, Aug. 7. Certain back-to-school purchases, such as clothing, school supplies, computers, and other items as defined by the statute, are exempt from sales tax for this time period only. Missouri Sales Tax Holiday 2016.