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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
In the other strand (lagging strand), as the forks opens, multiple sites of initiation are exposed. The synthesis, then proceed in short segments in the 5â-3â direction: that is, synthesis in the lagging strand is discontinuous. The short segments of DNA synthesized on the lagging strand are called Okazaki fragment. These fragments are about 1000 â 2000 nucleotide long.