If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
A safe and secure Missouri is one of the most basic building blocks upon which our state's future rests. To ensure security, the Missouri Office of Homeland Security was established on September 26, 2001. Missouri was the first state in the nation to create an Office of Homeland Security in response to the terrorist attacks of September 11, 2001.