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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
The last step in the synthesis of the lagging strand of DNA is the formation of a phosphodiester linkage between the 3â²-hydroxyl group at the end of one Okazaki fragment and the 5â²-phosphate group of an adjacent Okazaki fragment. This step is catalyzed by DNA ligase.