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A host of fractionation procedures are employed by cell biologists. Each organelle has characteristics (size, shape and density for example) which make it different from other organelles within the same cell.
Differential Centrifugation. By Sophie Legg. Differential Centrifugation This is the most common method of fractionating cells Fractionation is the separation of the different organelles within the cell Method: 1. Cut tissue in an ice-cold isotonic buffer.It is cold to stop enzyme reactions, isotonic to stop osmosis and a buffer to stop pH changes.y Sophie Legg. Differential Centrifugation This is the most common method of fractionating cells Fractionation is the separation of the different organelles within the cell Method: 1. Cut tissue in an ice-cold isotonic buffer.