[Hook: Ja Rule] + (Ashanti) Girl your stare, those eyes I (love it when you look at me baby) Your lips, your smile I (love it when you kiss me baby) Your hips, those thighs I (love it when you call me baby) And I can't, deny I (love it when I'm witchu baby) [Ja Rule] I, got a fetish for fuckin you witcha skirt on.
The primers used must be specific to the targeted sequences in the DNA of a virus, and the PCR can be used for diagnostic analyses or DNA sequencing of the viral genome. The high sensitivity of PCR permits virus detection soon after infection and even before the onset of disease.
Function of the RNA primer: DNA polymerases need a double-stranded DNA region to which they can attach in order to begin copying the rest of the DNA strand. In order to provide this double-stranded attachment site, RNA primers are added by primase, an RNA polymerase which does not require such an attachment site itself. When DNA replication starts, one primer is needed at the start of the leading strand.