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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
The lagging strand of DNA is that strand of the DNA double helix that is orientated in a 5' to 3' manner. Therefore, its complement must be synthesized in a 3'â5' manner. Because DNA polymerase III cannot synthesize in the 3'â5' direction, the lagging strand is synthesized in short segments known as Okazaki fragments.n eukaryotic primer removal, DNA polymerase Î´ extends the Okazaki fragment in 5' to 3' direction, and when it encounters the RNA primer from the previous Okazaki fragment, it displaces the 5â² end of the primer into a single-stranded RNA flap, which is removed by nuclease cleavage.