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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
The lagging strand is synthesized in short, separated segments. On the lagging strand template, a primase reads the template DNA and initiates synthesis of a short complementary RNA primer. A DNA polymerase extends the primed segments, forming Okazaki fragments. The RNA primers are then removed and replaced with DNA, and the fragments of DNA are joined together by DNA ligase.