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The resulting stretch of newly synthesized DNA that is associated with this lagging or discontinuous strand is called an Okazaki fragment. Okazaki fragments, as with DNA replication in general, requires RNA priming, which serves as the beginning of an Okazaki fragment.
Because the two nucleotide strands of DNA of DNA are antiparallel, replication takes place continuously on one strand (the leading stand) and discontinuously on the other ( the lagging strand). The short lengths of DNA produced by discontinous replication of the lagging strand are called Okazaki fragments. DNA Polymerase 1 cuts out the RNA primer and uses 5' to 3' polymerase activity to replace the RNA nucleotides with DNA nucleotides.