[Hook: Ja Rule] + (Ashanti) Girl your stare, those eyes I (love it when you look at me baby) Your lips, your smile I (love it when you kiss me baby) Your hips, those thighs I (love it when you call me baby) And I can't, deny I (love it when I'm witchu baby) [Ja Rule] I, got a fetish for fuckin you witcha skirt on.
The primers used must be specific to the targeted sequences in the DNA of a virus, and the PCR can be used for diagnostic analyses or DNA sequencing of the viral genome. The high sensitivity of PCR permits virus detection soon after infection and even before the onset of disease.
Primer sequences are determined from known sequence since there must be a match to the region of DNA to be amplified. PCR amplification requires 2 primers that determine the region of sequence amplified in the forward and reverse direction.ikewise, the reverse primer is designed from the complimentary strand. PCR is exponential amplification in which the newly generated PCR fragment from one cycle also acts as a template for the next cycle (Figure 1A). Amplification of DNA for Sanger sequencing differs from PCR in that a single primer is used.