If the primer concentration is calculated incorrectly, the calculated annealing temperature will also be incorrect. To calculate the primer T m, use the tool at www.basic.northwestern.edu/biotools/oligocalc.html with the default salt concentration and 0.2â1 ÂµM primer (depending on your reaction conditions).
Missouri Day. On March 22, 1915, the 48th General Assembly set aside the third Wednesday in October each year as Missouri Day, due to the efforts of Mrs. Anna Brosius Korn, a native Missourian. Missouri Day is a time for schools to honor the state and for the people of the state to celebrate the achievements of all Missourians.
Missouri's State Bird. On March 30, 1927, the native bluebird became the official state bird of Missouri. The bluebird, considered a symbol of happiness, is usually 6 1/2 to 7 inches long. While its upper parts are covered with light blue plumage, its breast is cinnamon red, turning rust-colored in the fall. The bluebird is common in Missouri from early spring until late November.