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Generally, laboratory mice become sexually mature between fi ve and eight weeks of age. Males of most. strains usually mature sexually by six weeks of age. DBA/2J (000671) and C3H/HeJ (000659) mice are precocious: females can. conceive when they are as young as 23 days old.
3. Expect litters within a month of mating since female mice go into estrus every 3 or 4 days and the gestation time of mice is 19-21 days.If no litters are produced after one month you should replace the mice that you are mating with your founder.. Expect litters within a month of mating since female mice go into estrus every 3 or 4 days and the gestation time of mice is 19-21 days.