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Generally, laboratory mice become sexually mature between fi ve and eight weeks of age. Males of most. strains usually mature sexually by six weeks of age. DBA/2J (000671) and C3H/HeJ (000659) mice are precocious: females can. conceive when they are as young as 23 days old.
You can sex a mice by looking at anogenital distance or the distance between the mouse's genital area and anus. Or observing whether or not your mouse has nipples. It's always best to know the sex of your mice so you can separate the males from the females and prevent unwanted pregnancies.