4. Once the nutrient agar has been autoclaved, allow it to cool but not solidify. 5. Pour nutrient agar into each plate and leave plates on the sterile surface until the agar has solidified. 6. Replace the lid of each Petri dish and store the plates in a refrigerator. Uses of Nutrients Agar. 1.
Bagon (Japanese: ã¿ããã¤ Tatsubay) is a Dragon-type Pokemon. PokÃ©mon it evolves Into shelgon starting at level, 30 which evolves Into salamence starting at level. 50
Pokemon Up-Grade   The Up-Grade is used to evolve a Porygon into Porygon2.   In FireRed to get a Porygon, you must get 9999 coins at the Rocket Game corner and then ex â¦ change them for a Porygon.  To get an Up-Grade, beat eight opponents in the Trainer Tower in Seven Island.nce the regular pokedex is upgraded to the national mode later in the game this will allow the pokedex to record pokemon from other regions which are Johto and Hoenn. An example is this: Charizard is a Kanto pokemon while Slugma is a Johto pokemon and Nincada is a Hoenn pokemon.