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Generally, laboratory mice become sexually mature between fi ve and eight weeks of age. Males of most. strains usually mature sexually by six weeks of age. DBA/2J (000671) and C3H/HeJ (000659) mice are precocious: females can. conceive when they are as young as 23 days old.
House stud males (individually) for 2 weeks prior to mating. Use 8-15 week old females. Add 1-2 female(s) into each stud maleâs cage in the late afternoon, prior to the dark cycle. Check for vaginal plugs early the next morning (plug will dissolve over time) The date a plug is observed is gestational day 0 to 0.5.