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Generally, laboratory mice become sexually mature between fi ve and eight weeks of age. Males of most. strains usually mature sexually by six weeks of age. DBA/2J (000671) and C3H/HeJ (000659) mice are precocious: females can. conceive when they are as young as 23 days old.
Mice should be weaned at 3 to 4 weeks after birth. Pups must be weaned if the same mom gives birth to a second litter. The pups should be robust, active, have open eyes, teeth and adult fur rather than the sparser fur of babies. They need to be able to jump up to the top of the cage to feed and drink.