The Method of Loci has been used since ancient times to memorize important information.
The NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit is a reagent-based protocol that enables the stepwise lysis of cells, separation of the cytoplasm from the intact nuclei and then extraction of nuclear proteins away from genomic DNA and mRNA.
There are three basic and two optional steps in a DNA extraction: 1  Cells which are to be studied need to be collected. 2  Breaking the cell membranes open to expose the DNA along with the cytoplasm within (cell lysis). Lipids from the cell membrane and the nucleus are broken down with detergents and surfactants. Breaking proteins by adding a protease (optional). Breaking RNA by adding an RNase (optional).