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In biochemistry and metabolism, beta-oxidation is the catabolic process by which fatty acid molecules are broken down in the cytosol in prokaryotes and in the mitochondria in eukaryotes to generate acetyl-CoA, which enters the citric acid cycle, and NADH and FADH2, which are co-enzymes used in the electron transport chain.
Fatty acid biosynthesis occurs similarly to Beta-oxidation-acetyl groups are added to a growing chain, but the mechanism of the pathway is distinctly different from being simply the reverse of Beta-oxidation (exception-elongation of palmitate in the mitochondrion-see below).ransport of Mitochondrial Acetyl-CoA into the Cytosol. Acetyl-CoA is produced in two ways in the mitochondria 1  -. by Beta-oxidation of fatty acids, and. 2  by combined action of pyruvate dehydrogenase (to decarboxylate pyruvate, producing acetate) and dihydrolipoyl transacetylase (to add the CoA to the acetate).