The Method of Loci has been used since ancient times to memorize important information.
The NE-PER Nuclear and Cytoplasmic Extraction Reagent Kit is a reagent-based protocol that enables the stepwise lysis of cells, separation of the cytoplasm from the intact nuclei and then extraction of nuclear proteins away from genomic DNA and mRNA.
Nuclear extract is collected by centrifugation at 12,000 g for 15 min at 4Â°C. Protein concentration of the nuclear extract is estimated using Bradfordâs reagent (BioRad, USA). The extract is either immediately used or stored at â70Â°C till further use.