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In biochemistry and metabolism, beta-oxidation is the catabolic process by which fatty acid molecules are broken down in the cytosol in prokaryotes and in the mitochondria in eukaryotes to generate acetyl-CoA, which enters the citric acid cycle, and NADH and FADH2, which are co-enzymes used in the electron transport chain.
Steps of Beta-Oxidation. Every good play has a plot. The main plot of beta-oxidation is that the fatty acids are looking to change themselves, or be broken down. Before we get to the main plot, a little bit of exposition is needed to set the scene.