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In biochemistry and metabolism, beta-oxidation is the catabolic process by which fatty acid molecules are broken down in the cytosol in prokaryotes and in the mitochondria in eukaryotes to generate acetyl-CoA, which enters the citric acid cycle, and NADH and FADH2, which are co-enzymes used in the electron transport chain.
Beta oxidation of fatty acids occurs in the peroxisomes (a eukaryotic organelle) in addition to the mitochondria, though in a modified form. A primary function of peroxisomal beta oxidation is to shorten very long chain fatty acids so they can be degraded in the mitochondrion.xidation of these branched fatty acids is accomplished by alpha oxidation. This process involves hydroxylation of the alpha carbon, removal of the terminal carboxyl group and concomitant conversion of the alpha hydroxyl group to a terminal carboxyl group, and linkage of CoA to the terminal carboxyl group.