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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
Intensity of staining. The original recommendations for a standard PAS were that both oxidation in 1% periodic acid and treatment with Schiff's reagent should be for about 10 minutes each.This gives distinct, but not especially intense, staining.he PAS reaction is quite straightforward. Sections are first brought to water, then a 1% aqueous solution of periodic acid is applied for 10 to 30 minutes. The sections are washed to remove any traces of the periodic acid, then the Schiff's reagent is applied for a while, again for 10 to 30 minutes.