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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
Haematoxylin is then typically used as a counter stain to visualise other tissue elements. When PAS is used to demonstrate fungal organisms, however, a light green counter stain is preferred. Diastase (alpha-amylase) digestion may also be used to assist in the diagnosis of glycogen storage diseases.Diastase hydrolyses starch, glycogen, and breakdown products originating in these tissue polysaccharides.2) Neutral mucus substances: It is also commonly used to stain and identify neutral mucus substances. These can include glycoproteins, glycolipids, and neutral mucins, which are produced by epithelial cells in different organs.