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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
4. Apply a solution of half hydrogen peroxide and half water to the stain with a spray bottle and a clean cloth. Don't rub or you may spread the stain. Blot the stain after letting the solution sit on the stain for a few minutes.Repeat the process if the entire stain is not removed the first time.. Apply a solution of half hydrogen peroxide and half water to the stain with a spray bottle and a clean cloth. Don't rub or you may spread the stain. Blot the stain after letting the solution sit on the stain for a few minutes.