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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
Figure 10: Periodic Acid Schiff (kidney). PAS staining is mainly used for staining structures containing a high proportion of carbohydrates such as glycogen,glycoproteins, proteoglycans typically found in connective tissues, mucus and basement membranes.n the histopathology laboratory, the term âroutine stainingâ refers to the hematoxylin and eosin stain (H&E) that is used âroutinelyâ with all tissue specimens to reveal the underlying tissue structures and conditions.