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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
intrinsic stain a stain that is within the enamel of a tooth and cannot be removed by polishing. metachromatic stain one that produces in certain elements a color different from that of the stain itself. nuclear stain one that selectively stains cell nuclei, generally a basic stain.