January 8, 2017 Avi Eberstein. In an interview last week on CNBCâs âPower Lunch,â former Piper Jaffray senior tech analyst turned venture capitalist Gene Munster said that virtual reality (VR), augmented reality (AR), artificial intelligence (AI) and robotics collectively are going to be a âbigger deal than the internet and mobile.â.
Importance. Plasmids come in many different sizes and are used for many different purposes in biotechnology. They first made their mark in the field of recombinant DNA in the 1970s, being used as a tool to insert genes into bacteria to encourage their production of therapeutic proteins such as human insulin.ble to self-replicate, plasmids can be picked up from the environment and transferred between bacteria. Plasmids are used by their host organism to cope with stress-related conditions. Many plasmids, for example, carry genes that code for the production of enzymes to inactivate antibiotics or poisons.
The pBR322 plasmid contains a gene that allow the bacteria to be resistant to the antibiotics tetracycline and amipicillin. To use pBR322 plasmid to clone a gene, a restriction endonuclease first cleaves the plasmid at a restriction site.pBR322 plasmid contains three restriction sites: PstI, SalI and ecoRI. The first two restriction sites are located within the gene that codes for ampicillin and tetracycline resistance, respectively.owever, plasmids are not limited to bacteria; they are also present in some eukaryotes (e.g., circular, nuclear plasmids in Dictyostelium purpureum). A plasmid is a circular, double stranded DNA that is usually found in bacteria (however it does occur in both eukarya and prokarya).