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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
In the staining process, those that decolorize easily are gram negative, and the one that retain the primary stain are gram positive.   The acid fast staining is a techique that stains only the gram positive and only the genuses of mycobacterium, nocardia, and actinomycetes.ram staining   Gram Staining is a way to separate one large groups of bacteria into two. Crystal violet is used to dye the cells, those that retain the color are grouped â¦ as Gram-positive, and those that do not retain the color are grouped as Gram-negative.