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PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans), typically found in e.g. connective tissues, mucus, the glycocalyx, and basal laminae.hese aldehydes then react with the Schiff reagent to give a purple-magenta color. A suitable basic stain is often used as a counterstain. PAS diastase stain (PAS-D) is PAS stain used in combination with diastase, an enzyme that breaks down glycogen.
1 Coomassie blue-stains proteins a brilliant blue, and is often used in gel electrophoresis. 2  Crystal violet-stains cell walls purple when combined with a mordant. 3  This stain is used in Gram staining. 4  DAPI-a fluorescent nuclear stain that is excited by ultraviolet light, showing blue fluorescence when bound to DNA. Hematoxylin-a nuclear stain that, with a mordant, stains nuclei blue-violet or brown. 2  Hoechst stains-two types of fluorescent stains, 33258 and 33342, these are used to stain DNA in living cells. 3  Iodine-used as a starch indicator. 4  When in solution, starch and iodine turn a dark blue color.