The major subject areas include: 1  The history of the franchise and its officers and directors. 2  A complete description of the business to be franchised. 3  All costs and fees that you will be subject to under the agreement. 4  All obligations of either party to the other during the term of the agreement and thereafter.
The information about collection date of the sample is critical and should be included. A precaution should be taken for the measurement of enzyme activity, related to the blood concentration in filter paper, which is not always constant. This concentration depends mainly on the sample's collection procedures [13] .ackground. Mucopolysaccharidosis IVA (MPS IVA), or Morquio Syndrome type A, is an autosomal recessive disease caused by deficiency of the lysosomal enzyme N-acetylgalactosamine-6-sulfatase (GALNS), resulting in excessive lysosomal storage of keratan sulfate in many tissues and organs.
A key criterion of the classical definition of an âevolutionarily optimized enzymeâ is thatk cat /K m should approach the substrate diffusion rate limit for enzyme-substrate bimolecular association .2 In the case of an enzyme such as DAGK which has two substrates, the rate limit will be defined by the substrate for which the rate of diffusion-controlled bimolecular association is lower.