If you want to file a petition for a writ of habeas corpus in the. Eastern District of Wisconsin, mail your completed original. petition, all attachments, and two copies of each (for a total of 3. complete sets of all documents), along with the $5.00 filing fee or.
Temperature and Humidity. For small plants, just say the first three weeks a temperature of between 20 and 25 degrees is ideal.
The annealing temperature is typically 2-3 degrees Celsius below the melting temperature of the primers. In the annealing step, the polymerase binds to the primer-template hybrid and begins DNA formation. The last step in PCR is the elongation step.