Organic acids are ubiquitous throughout the field of chemistry. Whether used as a solvent, a substrate, a strong acid, or in a catalytic amount as a Bronsted acid source, organic acids are as commonplace as glassware for the researcher.
In the equation, p 2 represents the frequency of the homozygous genotype AA, q 2 represents the frequency of the homozygous genotype aa, and 2pq represents the frequency of the heterozygous genotype Aa. In addition, the sum of the allele frequencies for all the alleles at the locus must be 1, so p + q = 1.
For the following discussion: Variant allele frequency = read count supporting mutant base / total read count at that position For heterozygous somatic variants we can expect a tumor variant allele frequency of 50% and a normal variant allele frequency of 0%.Of course there will always be some variability due to some of the technical artifacts already discussed (random sequencing errors, etc.).n this case somatic variants that exist only in the sub-clone will have a substantially reduced tumor variant allele frequency. When both purity and heterogeneity are in play, interpreting tumor variant allele frequencies just gets more and more complicated ...