If you want to file a petition for a writ of habeas corpus in the. Eastern District of Wisconsin, mail your completed original. petition, all attachments, and two copies of each (for a total of 3. complete sets of all documents), along with the $5.00 filing fee or.
Temperature and Humidity. For small plants, just say the first three weeks a temperature of between 20 and 25 degrees is ideal.
T m (primer) = Melting Temperature of the primers. T m (product) = Melting temperature of the product. 4. GC Content: The GC content (the number of G's and C's in the primer as a percentage of the total bases) of primer should be 40-60%.5. m of product is the melting temperature of the PCR product. 5. Primer Pair Tm Mismatch Calculation: The two primers of a primer pair should have closely matched melting temperatures for maximizing PCR product yield.