Organic acids are ubiquitous throughout the field of chemistry. Whether used as a solvent, a substrate, a strong acid, or in a catalytic amount as a Bronsted acid source, organic acids are as commonplace as glassware for the researcher.
where p is the frequency of the A allele and q is the frequency of the a allele in the population. In the equation, p 2 represents the frequency of the homozygous genotype AA, q 2 represents the frequency of the homozygous genotype aa, and 2pq represents the frequency of the heterozygous genotype Aa.
For the following discussion: Variant allele frequency = read count supporting mutant base / total read count at that position For heterozygous somatic variants we can expect a tumor variant allele frequency of 50% and a normal variant allele frequency of 0%.Of course there will always be some variability due to some of the technical artifacts already discussed (random sequencing errors, etc.).n this case somatic variants that exist only in the sub-clone will have a substantially reduced tumor variant allele frequency. When both purity and heterogeneity are in play, interpreting tumor variant allele frequencies just gets more and more complicated ...