If you want to file a petition for a writ of habeas corpus in the. Eastern District of Wisconsin, mail your completed original. petition, all attachments, and two copies of each (for a total of 3. complete sets of all documents), along with the $5.00 filing fee or.
Temperature and Humidity. For small plants, just say the first three weeks a temperature of between 20 and 25 degrees is ideal.
Notes. 1  In the actual PCR run use an annealing temperature 5Â°C lower than the lower Tm given by the calculator. 2  If high DMSO concentration is used, the annealing temperature determined by the guidelines above must be lowered, as DMSO decreases the melting point of the primers. If high DMSO concentration is used, the annealing temperature determined by the guidelines above must be lowered, as DMSO decreases the melting point of the primers. 2  It has been reported that 10% DMSO decreases the melting temperature by 5.5â6.0Â°C. 2.