If you want to file a petition for a writ of habeas corpus in the. Eastern District of Wisconsin, mail your completed original. petition, all attachments, and two copies of each (for a total of 3. complete sets of all documents), along with the $5.00 filing fee or.
Temperature and Humidity. For small plants, just say the first three weeks a temperature of between 20 and 25 degrees is ideal.
1. Primer Length: It is generally accepted that the optimal length of PCR primers is 18-22 bp. This length is long enough for adequate specificity and short enough for primers to bind easily to the template at the annealing temperature. 2. m of product is the melting temperature of the PCR product. 5. Primer Pair Tm Mismatch Calculation: The two primers of a primer pair should have closely matched melting temperatures for maximizing PCR product yield.