If you want to file a petition for a writ of habeas corpus in the. Eastern District of Wisconsin, mail your completed original. petition, all attachments, and two copies of each (for a total of 3. complete sets of all documents), along with the $5.00 filing fee or.
Temperature and Humidity. For small plants, just say the first three weeks a temperature of between 20 and 25 degrees is ideal.
1 The optimal temperature for Taq polymerase is about 75-80 o C, but it is partially active even at typical annealing temperatures so primer extension begins during the annealing step. 2  The rate of primer extension by Taq polymerase is about 50-100 nucleotides/sec.