The major subject areas include: 1  The history of the franchise and its officers and directors. 2  A complete description of the business to be franchised. 3  All costs and fees that you will be subject to under the agreement. 4  All obligations of either party to the other during the term of the agreement and thereafter.
The information about collection date of the sample is critical and should be included. A precaution should be taken for the measurement of enzyme activity, related to the blood concentration in filter paper, which is not always constant. This concentration depends mainly on the sample's collection procedures [13] .ackground. Mucopolysaccharidosis IVA (MPS IVA), or Morquio Syndrome type A, is an autosomal recessive disease caused by deficiency of the lysosomal enzyme N-acetylgalactosamine-6-sulfatase (GALNS), resulting in excessive lysosomal storage of keratan sulfate in many tissues and organs.
4. Blood collection. Blood samples are collected for measurement of blood lipids and glucose. For lipid measurements, serum should be used in preference to plasma to avoid the diluting effect of anticoagulants, which results in about 3% difference in concentrations.entrifuging. If samples for glucose measurements are taken, the samples should be centrifuged no later than 20-35 minutes after the sample is drawn. For serum samples, blood should be centrifuged within one hour after blood collection.