DNA purity (A 260 /A 280) = (A 260 reading â A 320 reading) Ã· (A 280 reading â A 320 reading). Strong absorbance around 230nm can indicate that organic compounds or chaotropic salts are present in the purified DNA. A ratio of 260nm to 230nm can help evaluate the level of salt carryover in the purified DNA.otal yield is obtained by multiplying the DNA concentration by the final total purified sample volume. DNA yield (Âµg) = DNA concentration Ã total sample volume (ml). However, DNA is not the only molecule that can absorb UV light at 260nm.
Automatism: An unconscious movement that may resemble simple repetitive tics or may be a complex sequence of natural-looking movements. This curious type of behavior occurs in a number of neurological and psychiatric disorders. The neurologic disorders associated with automatisms include narcolepsy (the sudden recurrent uncontrollable compulsion to sleep) and some forms of epilepsy such as especially psychomotor epilepsy. The psychiatric conditions associated with automatisms include schizophrenia (catatonic type) and fugue (flight) states. Automatisms involve doing something automatically and not remembering afterwards how one did it or even that one did it. Automatisms are also called automatic behavior.
Automatism is a rarely used criminal defence. It is one of the mental condition defences that relate to the mental state of the defendant. Automatism can be seen variously as lack of voluntariness, lack of culpability or excuse. Automatism means that the defendant was not aware of his or her actions when making the particular movements that constituted the illegal act. For example, Esther Griggs in 1858 threw her child out of a first floor window believing that the house was on fire, while havin