Networking. Organizations have a networking relationship when they exchange information in order to help each organization do a better job. For example, if a school and a community counseling center share information about their counseling services for youth, that is a form of networking.
Phenolâchloroform extraction is a liquid-liquid extraction technique in biochemistry and molecular biology for purifying nucleic acids and eliminating proteins. In brief, aqueous samples are mixed with equal volumes of a phenol: chloroform mixture.After mixing, the mixture is centrifuged and two distinct phases are formed, because the phenol: chloroform mixture is immiscible with water.n brief, aqueous samples are mixed with equal volumes of a phenol: chloroform mixture. After mixing, the mixture is centrifuged and two distinct phases are formed, because the phenol: chloroform mixture is immiscible with water.
Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.