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However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University.Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern.ith northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions.
Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.The method is named after its inventor, the British biologist Edwin Southern.Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but using RNA or protein, have later been named in reference to Edwin Southern's name.As the label is eponymous, Southern is capitalised, as is conventional for proper nouns. The DNA fragments are then electrophoresed on an agarose gel to separate them by size. 2  If some of the DNA fragments are larger than 15 kb, then prior to blotting, the gel may be treated with an acid, such as dilute HCl.