The Senate approved the legislation earlier Wednesday in a 78-20 vote, and Obama signed it into law that evening. All 20 ânoâ votes in the Senate came from Republicans, though Sens. Marco Rubio (R-Fla.) and Lindsey Graham (R-S.C.), both running for president, missed the vote. Without action by Congress, the federal government would have shut down on Thursday, the beginning of a new fiscal year.
A buffer is made by mixing a large volume of a weak acid or weak base together with its conjugate. A weak acid and its conjugate base can remain in solution without neutralizing each other.
All Answers (3) 1  Dear Elizabeth. TAE buffer is a buffer containing a mixture of Tris base, acetic acid and EDTA. 2  For DNA storage, you could use TE (Tris 10mM, EDTA 10mM, pH7.4) or water. If you use TE, in PCR reaction, you might have some inhibition, if you use to much volume of your sample.