03 Completion Report Instructions.pdf. 8000-FM-OOGM0004b. 02/19/16: 44 KB: Delete Document; Permissions
This magnification value determines the diameter of the field of view when the low power objective is used. A microscope's field of view refers to the diameter of the circular optical light that is visible when viewing an object under the microscope. This value forms an inverse relationship with magnification, which means that increasing the magnification results in the reduction of the field of view.
The fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image. sample is treated with a primary antibody specific for the molecule of interest. A fluorophore can be directly conjugated to the primary antibody. Alternatively a secondary antibody, conjugated to a fluorophore, which binds specifically to the first antibody can be used.