The ELISA (enzyme-linked immunosorbent assay) is a test which uses color change and antibodies to identify a substance. An indirect ELISA is one of three ELISA tests.
Urine varies in appearance, depending principally upon a body's level of hydration, as well as other factors. Normal urine is a transparent solution ranging from colorless to amber but is usually a pale yellow. In the urine of a healthy individual the color comes primarily from the presence of urobilin.
The yellow color in normal urine comes from a substance known as urochrome. Urochrome is also known as urobilin and is the result of hemoglobin breakdown. Hemoglobin is the protein in red blood cells that enables oxygen to be taken around the body.