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Overview of mitosis: During the course of the cell cycle interphase and mitosis alternate. During interphase the chromosomes replicate and the cell prepares for mitosis, which is the process of cell division in which one cell becomes two genetically identical daughter cells. During interphase the chromosomes exist as thin, unpacked threads invisible under a light microscope.
Best Answer: Chromosomes don't duplicate during mitosis. They duplicate, or double, during a process called replication. Replication occurs in the nucleus during interphase, which is prior to mitosis.