03 Completion Report Instructions.pdf. 8000-FM-OOGM0004b. 02/19/16: 44 KB: Delete Document; Permissions
This magnification value determines the diameter of the field of view when the low power objective is used. A microscope's field of view refers to the diameter of the circular optical light that is visible when viewing an object under the microscope. This value forms an inverse relationship with magnification, which means that increasing the magnification results in the reduction of the field of view.
Light diffracted by the specimen is presented as an inverted cone of half-angle (Î±), which represents the limits of light that can enter the objective. In order to increase the effective aperture and resolving power of the microscope, a condenser (Figure 1(b)) is added to generate a ray cone on the illumination side of the specimen. This enables the objective to gather light rays that are the result of larger diffraction angles, increasing the resolution of the microscope system. The sum of the aperture angles of the objective and the condenser is referred to as the working aperture.