03 Completion Report Instructions.pdf. 8000-FM-OOGM0004b. 02/19/16: 44 KB: Delete Document; Permissions
This magnification value determines the diameter of the field of view when the low power objective is used. A microscope's field of view refers to the diameter of the circular optical light that is visible when viewing an object under the microscope. This value forms an inverse relationship with magnification, which means that increasing the magnification results in the reduction of the field of view.
Play media. Fluorescent and confocal microscopes operating principle. A fluorescence microscope is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to study properties of organic or inorganic substances. The fluorescence microscope refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image.