1B) (Matson 1986). Polarity is ascertained by identifying the duplex region that is denatured by the helicase. For helicases involved in DNA replication, the polarity of the reaction is strongly indicative of helicase placement on the leading (a 3 ' -5 'polarity) or lagging (a 5 +3 'polarity) strand.NA HELICASE ASSAYS DNA helicases are often first detected by a DNA-dependent or DNA-stimulated NTPase activity. Verification of DNA helicase activity is then accomplished using an assay that reveals the conversion of double-stranded DNA (dsDNA) into two single strands.
1. typical enlistment is 4 years, however during Vietnam 3 year enlistments were allowed. 2. The 3rd Marine Division was reactivated for Korea, and in 1956 moved to Okinawa as the cease fire there looked like it would hold. Air Bases remained active in Okinawa during the entire postwar and Cold War era to date.
It was apparently based on an estimate that 800,000 Vietnam-era veterans had died by 2000. That number was reasonable: About 9.2 million Americans served in the military during the Vietnam era (1964-75), so that would mean about 8 percent of them had died and 92 percent were still alive.