The LSRPA is a non-profit professional membership organization established to provide information, education and technical resources to LSRPs, other professionals involved in environmental remediation in the State of New Jersey and students pursuing an education in a discipline related to environmental remediation.
Western blotting and enzyme-linked immunosorbent assay (ELISA) are widely used in the protein detection (Savige, 1998). The name of Western blotting, it is also called Western blot. ELISA is the abbreviation of enzyme-linked immunosorbent assay (Ma and Shieh, 2006).
Western blot using an antibody that recognizes proteins modified with lipoic acid. The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract.It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide.he proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.