The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
Minnesota Monthly Accelerated Sales Tax Payments Suspended. Minnesota Accelerated Payments Program Suspended. The Minnesota Department of Revenue has announced that âEffective July 1, 2012, monthly accelerated payments will be suspended, with the exception of the June estimated payment.â. Currently, businesses with a sales tax liability of $120,000 or more in the previous fiscal year, are required to make accelerated payments.