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In genetics, a transcription terminator is a section of nucleic acid sequence that marks the end of a gene or operon in genomic DNA during transcription.
Encoded in the DNA is some type of index that directs the molecular machinery to the start of the hemoglobin gene. When new hemoglobin is required, protein production is activated. The gene is read and the protein is manufactured. At the end of the gene is a message called a stop codon, which ends protein production. The manufacture of proteins using the instructions from the gene is called protein expression. Doug Prasher envisioned that it would be possible to use biomolecular techniques to insert the GFP gene at the end of the hemoglobin gene, right before the stop codon.