Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
The young accompany the female until they are old enough to breed. About half of the young females in Missouri breed in the year of their birth. Other females and young males breed first at 1Â½ years of age. Deer are in the prime of life between 2Â½ and 7Â½ years of age. Deer can live for 15 years in the wild and 25 years in captivity.