The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
You must pay the Minnesota AMT if the âalternative taxâ on line 27 of Schedule M1MT exceeds the âregular taxâ on line 28. What is the Alternative Minimum Tax rate? Beginning with tax year 2013, the rate is 6.75%.