The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
The City of Cambridge FY2015 real estate and personal property tax bills will be mailed on October 17, 2014. The first half portion is due and payable on November 17, 2014. The second half portion most likely will be due and payable on May 1, 2015. Pay Real Estate and Personal Property Tax bills online for a convenience fee. Learn More. Overvaluation abatement applications are due November 17, 2014. Learn more about the overvaluation abatement and to download an abatement application form.