The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
Due Date. The due date for filing your Homestead Credit Refund (for Homeowners) return (Form M1PR) is Aug. 15. If the due date falls on a weekend or legal holiday, your return is due by the next business day. Note: You may file Form M1PR and claim a refund for up to one year after the original due date.