Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
JEFFERSON CITY, Mo. The fall seasons have just ended, and Missouri hunters now can mark their calendars for next year. The Missouri Conservation Commission approved recommendations by the Missouri Department of Conservation (MDC) for 2017-2018 turkey-hunting and deer-hunting season dates. 2017 SPRING TURKEY HUNTING DATES.