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Originally, the native American populations along the Amazon spoke languages belonging to the Tupi-Guarani, Arawakan and Carib language families. It is thought that tribes speaking Macro-GÃª languages were driven out of the Amazon region into the Brazilian highlands shortly before contact with Europeans.
Over 300 have been documented in the Amazon basin (Aikhenvald 2012), but as Sophia de Tricht said, there are still uncontacted people. It is not impossible that many of those groups speak languages that are the same as one of the 300 we have documented so far, but it is unlikely that the majority do. Aikhenvald, A. I., & Aikhenvald, A. Y. (2012). Languages of the Amazon. Oxford University Press.