The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
The June accelerated payment is due two business days before June 30. The remaining payment and your June return are due Aug. 20. To avoid penalty, your June accelerated payÂ­ment must be at least: 81.4 percent of your actual June liability, or. 81.4 percent of your May liability, or. 81.4 percent of your average monthly liabilÂ­ity for the previous calendar year.