Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Sept. 13: Opening early mountain quail season, and tree squirrel season. Sept. 13: Opening of general hunting season for blue and ruffed grouse. Sept. 15: Closing of dove season, first half. Sept. 20: Opening of band-tailed pigeon season in northern California. Sept. 20: Opening of general deer season zones B-1, B-2, B-3, B-5, B-6, C-1, C-2, C-3, C-4, D-6 and D-7. Sept. 20-21: Special Youth Hunt Days for waterfowl in the Northeastern California Zone. Sept. 21: Closing of general deer season zone A, South Unit 110 and North Unit 160. Sept. 27: Opening of early coastal quail season (Q2). Sept. 27: Opening of general deer season zone D-3, D-4, D-5, D-8, D-9 and D-10.