Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Missouri Sales Tax Holiday 2016. By Missouri state law, the sales tax holiday begins on the first Friday in August and continues through the following Sunday. In 2016, the three-day holiday begins at 12:01 a.m. on Friday, Aug. 5, and runs through Sunday, Aug. 7. Certain back-to-school purchases, such as clothing, school supplies, computers, and other items as defined by the statute, are exempt from sales tax for this time period only. Missouri Sales Tax Holiday 2016.