The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
When to file. April 15 of each year is the due date for filing your federal individual income tax return if you are a calendar year filer whose tax year ends on December 31. Your return is considered filed timely if the envelope is properly addressed and postmarked no later than April 15.ou will owe interest on any past-due tax and you may be subject to a late-payment penalty if the payment of tax is not timely (by your due date). To receive an automatic 6-month extension of time to file your return, you can file Form 4868 (PDF) by the due date of your return. See Topic 304 for more information.