Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Missouri is blessed with a great variety of trees, shrubs, and vines. Their leaves turn at different times, so Missourians enjoy a fall color season that may last four to six weeks. Sassafras, sumac, and Virginia creeper are some of the earliest to change, beginning in mid-September.