March 31, 2015. A server, regardless of the type, needs a robust network to give it access to the Internet and allow it to communicate with other computers. Since a server normally has exceptionally high traffic loads when compared to a normal workstation or desktop PC, it requires a network structure robust enough to prevent a bottleneck.
DNA ligase reconnects DNA strands when they are broken. It uses a cofactor molecule (shown in red) for power and a special lysine amino acid (shown in magenta) to perform the reaction. Our DNA ligases and the DNA ligase from the bacteriophage T7 (shown at the top from PDB entry 1a0i ) use ATP as the cofactor. Many bacteria, on the other hand, use NAD in the reaction, as with the DNA ligase shown at the bottom (from PDB entry 1dgs ). In both cases, a lysine in the DNA ligase forms a bond to the phosphate in the cofactor, holding onto the AMP portion and discarding the rest. Later in the reaction, this AMP is transferred to the broken DNA strand, and then is released when the strand is rejoined.
DNA ligase is an enzyme that has a very critical role in the process of DNA replication and DNA repair. There are two different things going on when DNA is replicated. Let's revisit this process very briefly.NA ligase is similar to the construction crew that builds the bridges. Through the process of DNA replication, many fragments are produced. DNA ligase fills the spaces between the different fragments to bridge together the gaps in DNA.