Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Eastern Bluebird The official state bird of Missouri is the Eastern Bluebird, adopted in 1927. The bluebird is the official state bird for Missouri. Eastern Bluebird The official state bird of Missouri is the Eastern Bluebird, adopted in 1927. The bluebird is the official state bird for Missouri.