Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Related: Tennessee hunter bags state, possible world-record, deer. Hunters are reminded there are mandatory procedures they need to follow if they harvest a deer on the seasonâs opening weekend in any of the 29 counties that comprise the Missouri Department of Conservationâs CWD Management Zone.