The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
It is due August 15, 2015. Send it to: Minnesota Property Tax Refund St. Paul, MN 55145-0020. ï· You should get your refund within 90 days after August 15, 2015. ï· You can get your refund up to 30 days earlier if you file electronically and do the following things: Â­ File by May 15 th if you rent or own a mobile home.