The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
Electronic tax returns usually are processed within 35 days. If you have been waiting for more than six weeks, you might want to contact the State to verify they received your return. You can check the status of your refund online. See related links. Minnesota Tax Questions: 651-296-3781 800-652-9094 indinctax@state.mn.us.