Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Groundhog. Closed for deer gun season only. No Limit. It is unlawful to hunt any wild animal except deer, coyote, waterfowl, or feral swine (wild boar) during the seven-day deer gun season, November 30 - December 6, 2015 between 30 minutes before sunrise to 30 minutes after sunset.