Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
Aug 20, 2015. JEFFERSON CITY, Mo. â The Missouri Conservation Commission set season dates and limits for the 2016-2017 fall deer season and gave initial approval to several deer-hunting regulation changes proposed by the Missouri Department of Conservation (MDC) at the Commission's Aug. 19 meeting in Jefferson City.