Melting temperature is that temperature at which the DNA duplexes will be single stranded (both of tempelate and primer). Annealing temperature is that temperature at which the single stranded primer will specifically bind to the template sequence. So it has to be lower than the melting temperature.
Missouri deer hunters will see changes in 2016. The voice of Missouri deer hunters has been heard. The Conservation Commission set the 2016-17 deer season at a meeting in August, and among the changes were a regular firearms season that was reduced from 11 to nine days.
In a recent study on Missouri National Guard bases, ticks were more than 3 times more numerous in May-June than in July-September. Immature (nymphal) ticks were more abundant (76%) than were adults (24%) (Barnhart 1999).