The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal. The most important values for estimating the Ta is the primer quality, the Tm of the primers and the length of PCR fragment.
Minnesota Sales Tax Law â New Requirement for Monthly Payment Due Dates. August 5th, 2010. A new law requires those who are currently required to file June accelerated payments of sales and use tax must also pay a portion of each monthâs tax liability early, beginning September 2010.
Recent History of June Accelerated Payments (Source: 2012 MN Tax Handbook) 1981 Enacted at 50% for Sales Tax for 1982 liabilities. 1986 Enacted at 50% for Cigarette Taxes, Tobacco Taxes, and Alcohol Beverage Taxes for 1987 liabilities. 1993 Increased from 50% to 75% for Sales Tax for 1994 liabilities.