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Within eukaryotes, DNA replication is controlled within the context of the cell cycle. As the cell grows and divides, it progresses through stages in the cell cycle; DNA replication takes place during the S phase (synthesis phase).
The chromosomes and genes contained within DNA are replicated during the S phase of the interphase cell cycle. DNA replication occurs within the nucleus of eukaryotes and in the cytoplasm of prokaryotes. DNA replication occurs in all living organisms.