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Typically, it is possible to detect methadone in the urine for 2 to 4 days after it has been used, which implies that this is the period for which methadone stays in your system. On the other hand, the detection window of methadone in blood is approximately 24 hours, while that in saliva is about 1 to 10 days.
Methadone has an elimination half-life of 8 to 59 hours. Unlike other drugs, methadone has a wide bioavailability window varying from 40 to 99%. Up to 90% of methadone binds with blood plasma but after repetitive administration these number drastically change.