T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75â80 Â°C, with a half-life of greater than 2 hours at 92.5 Â°C, 40 minutes at 95 Â°C and 9 minutes at 97.5 Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 Â°C.
Glucose and fructose are monosaccharides and are the building blocks of sucrose, a disaccharide. Thus, disaccharides are just a pair of linked sugar molecules. They are formed when two monosaccharides are joined together and a molecule of water is removed -- a dehydration reaction.
2.like glucose, fructose,galactose. disaccharides. 1. are two monosaccharides, two monosaccharides forms disaccharides after condensation (removing a molecule of water). 2. like sucrose, lactose, maltose.polysaccharides. 1. are union of many many mono-or disaccharides after condensation or removing of a molecule of water at each linkage point.he monosaccharides include glucose, fructose, and galactose. The disaccharides are two carbon rings bonded together by a glycosidic linkage in a condensation (dehydration) reaction, which removes a molecule of water.