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Within eukaryotes, DNA replication is controlled within the context of the cell cycle. As the cell grows and divides, it progresses through stages in the cell cycle; DNA replication takes place during the S phase (synthesis phase).
Replication occurs during interphase. Interphase is the stage of the cell cycle in which cells spend most (typically, more than 90%) of their time and perform their customary functions, including preparation for cell division. When this phase begins, the chromosomes have not yet replicated, but by the beginning of prophase replication is complete, so that each chromosome is composed of two sister chromatids.