T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75â80 Â°C, with a half-life of greater than 2 hours at 92.5 Â°C, 40 minutes at 95 Â°C and 9 minutes at 97.5 Â°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 Â°C.
Glucose and fructose are monosaccharides and are the building blocks of sucrose, a disaccharide. Thus, disaccharides are just a pair of linked sugar molecules. They are formed when two monosaccharides are joined together and a molecule of water is removed -- a dehydration reaction.
Sucrose. Sucrose is a disaccharide, which is a complex carbohydrate molecule that is made up of two monosaccharide sugars bonded together. Both of the monosaccharides in sucrose are present in their component forms in nature. These monosaccharides are glucose and fructose.ructose. Fructose is a sugar present in most berries, tree fruits, and melons, as well as honey. It is a simple reducing sugar, which means that it is capable of reducing the amount of chemicals in its structure through oxidation.