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Unicellular organisms are composed of a single cell. Multicellular organisms are composed of many specialized cells. Specialized cells differ in structure (size, shape...) and function (the role they perform in the organism).
Multicellular organisms, as the name implies, have many types of cells, while unicellular organisms contain just one cell each. Both kinds of organisms reproduce through meiosis or mitosis. Multicellular organisms generally form the higher tiers in the web of life.