Gel Analysis of Bisulfite Converted DNA. When using an agarose gel to analyze the converted DNA, use a 2% gel with a 100 bp marker. When using gels it can be necessary to load up to 100 ng of sample to visualize the DNA. Many researchers worry when at first they canât see any band in their gel.
Converting PST to EST. This time zone converter lets you visually and very quickly convert PST to EST and vice-versa. Simply mouse over the colored hour-tiles and glance at the hours selected by the column... and done! PST stands for Pacific Standard Time. EST is known as Eastern Standard Time. EST is 3 hours ahead of PST.
The difference between EST and GMT is -5 hours, so if it is 5.00pm EST is will be 10.00pm GMT. However you could have also meant Eastern Standard Time by using EST.