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100% activity is attained after 24 hours. It is also described therein that, 6 hours after oral administration to cats, nitenpyram reaches an activity of 60% against the fleas present on the cats, and on the second day after renewed infestation it still gives 55% activity.
Plan B One-Step (as well as its generic alternatives) is most effective if you use it within 24 hours. It can be taken up to 72 hoursâthough research suggests that these emergency contraceptives may still be effective for up to 5 days or 120 hours.