Gel Analysis of Bisulfite Converted DNA. When using an agarose gel to analyze the converted DNA, use a 2% gel with a 100 bp marker. When using gels it can be necessary to load up to 100 ng of sample to visualize the DNA. Many researchers worry when at first they canât see any band in their gel.
Converting PST to EST. This time zone converter lets you visually and very quickly convert PST to EST and vice-versa. Simply mouse over the colored hour-tiles and glance at the hours selected by the column... and done! PST stands for Pacific Standard Time. EST is known as Eastern Standard Time. EST is 3 hours ahead of PST.
If we were converting between these two time zones, there would be a two hour difference. PDT is seven hours behind Coordinated Universal Time (UTC) so it is written as UTC -7:00. EST is five hours behind UTC, written as UTC -5:00. Hereâs an example: If it is 2:15 PM in Pacific Daylight Time, then it is 4:15 PM in Eastern Standard Time. This gap widens when both zones are off of DST in the winter months. When both standard times are in effect, the difference is three hours.