Gel Analysis of Bisulfite Converted DNA. When using an agarose gel to analyze the converted DNA, use a 2% gel with a 100 bp marker. When using gels it can be necessary to load up to 100 ng of sample to visualize the DNA. Many researchers worry when at first they canât see any band in their gel.
Converting PST to EST. This time zone converter lets you visually and very quickly convert PST to EST and vice-versa. Simply mouse over the colored hour-tiles and glance at the hours selected by the column... and done! PST stands for Pacific Standard Time. EST is known as Eastern Standard Time. EST is 3 hours ahead of PST.
The Difference Between PDT and PST. During the summer, parts of the world, the United States mostly, observe Daylight Saving Time where the clocks are turned back an hour to allow for more sunlight during the summer months. During this time the offset for this time zone is UTC -7:00. When the winter months arrive and the clocks are set back to their original time, the zone is then designated as Pacific Standard Time (PST). During the winter months when PST is in effect, the offset is UTC -8:00. Understanding this distinction will help you better convert between time zones, but if youâd rather not do the math, you can also use our extremely helpful time zone converter to do the job for you.