A double blind study is the most rigorous clinical research design because, in addition to the randomization of subjects, which reduces the risk of bias, it can eliminate or minimize the placebo effect which is a further challenge to the validity of a study.
Transcription by RNA polymerase. An RNA strand is synthesized in the 5â²âââ3â² direction from a locally single stranded region of DNA. (After E. J. Gardner, M. J. Simmons, and D. P. Snustad, Principles of Genetics, (more...).igure 10-6. Transcription of two genes. (a) RNA polymerase moves from the 3â² end of the template strand, creating an RNA strand that grows in a 5â²âââ3â² direction (because it must be antiparallel to the template strand).
The newly made lagging strand still is synthesized 5'-3'. However, since the DNA is oriented in a manner that does not allow continual synthesis, only small sections can be read at a time. An RNA primer is placed on the DNA strand 3' to the origin of replication.Just as before, DNA Polymerase reads 3'-5' on the original DNA to produce a 5'-3' nascent strand. Polymerase reaches the origin of replication and stops replication until a new RNA primer is placed 3' to the last RNA primer.he newly made lagging strand still is synthesized 5'-3'. However, since the DNA is oriented in a manner that does not allow continual synthesis, only small sections can be read at a time. An RNA primer is placed on the DNA strand 3' to the origin of replication.