A double blind study is the most rigorous clinical research design because, in addition to the randomization of subjects, which reduces the risk of bias, it can eliminate or minimize the placebo effect which is a further challenge to the validity of a study.
Transcription by RNA polymerase. An RNA strand is synthesized in the 5â²âââ3â² direction from a locally single stranded region of DNA. (After E. J. Gardner, M. J. Simmons, and D. P. Snustad, Principles of Genetics, (more...).igure 10-6. Transcription of two genes. (a) RNA polymerase moves from the 3â² end of the template strand, creating an RNA strand that grows in a 5â²âââ3â² direction (because it must be antiparallel to the template strand).
DNA replication occurs in the 5' to 3' direction because 3' to 5' replication is chemically impossible. Explain your answer. Lagging strand synthesis is more complex because of the requirement for DNA replication to take place in the 5' to 3' direction.Since parent strands are oriented in an anti-parallel fashion, one strand is oriented in the 5' to 3' direction, while the other strand is oriented in the 3' to 5' direction.hile DNA replication does occur in the 5' to 3' direction, the reason is not because 3' to 5' replication is chemically impossible. 3' to 5' replication can in principle occur. The 3' OH of the incoming nucleotide instead of the nucleotide attached to the end of the growing chain would be the attacking group.