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Most of the websites i have looked at, say that the frequency of middle C note is approximately 261.626 Hz. and that middle C on guitar is at the 5th string 3rd fret. but my tuner says the frequency at the 5th string 3rd fret is 130.81 Hz.
A Quick Note About Middle C. The issue of middle C on a guitar can lead to some confusion, so let's iron that out before we get into actually tuning up. Middle C on a standard 88 key piano is the 4th C note you'll come to as you move across the keyboard from the left side.