A double blind study is the most rigorous clinical research design because, in addition to the randomization of subjects, which reduces the risk of bias, it can eliminate or minimize the placebo effect which is a further challenge to the validity of a study.
Transcription by RNA polymerase. An RNA strand is synthesized in the 5â²âââ3â² direction from a locally single stranded region of DNA. (After E. J. Gardner, M. J. Simmons, and D. P. Snustad, Principles of Genetics, (more...).igure 10-6. Transcription of two genes. (a) RNA polymerase moves from the 3â² end of the template strand, creating an RNA strand that grows in a 5â²âââ3â² direction (because it must be antiparallel to the template strand).
Synthesis of the lagging strand is accomplished through the repetition of the following steps. 1  Step 1: A new fragment begins with DNA polymerase III binding to the 3' end of the most recently produced RNA primer, primer B in this case, which is closest to the replication fork. 1  DNA pol III then adds DNA nucleotides in the 5' to 3' direction until it encounters the previous RNA primer, primer A.