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Osmolality of blood increases with dehydration and decreases with overhydration. In normal people, increased osmolarity in the blood will stimulate secretion of antidiuretic hormone (ADH). This will result in increased water reabsorption, more concentrated urine, and less concentrated blood plasma.
Sodium, potassium and chloride are the major ions involved in the regulation of osmotic pressure in body fluids. Osmolarity is a measure of the osmotic pressure and is determined by the number of particles dissolved in those fluids. The osmolarity of extra cellular fluid (ECF) is approximately 300 milliosmols/liter.