Although the same diameter, the Mark 14 was longer, at 20 ft 6 in (6.25 m), and therefore incompatible with older submarines' 15 ft 3 in (4.65 m) torpedo tubes. The Mark 14 was designed at the Naval Torpedo Station (NTS), Newport, beginning in 1922. It had a fairly small warhead and was intended to explode beneath the keel where there was no armor.
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The enzyme-linked immunospot (ELISPOT) assay is a widely used method for monitoring immune responses. The assay is a highly sensitive method for the ex vivo quantification of cytokine or antibody secreting cells after stimulation with an appropriate stimulus in vitro (Cox et al.2006).oreover the assay is relatively fast, highly efficient and can detect a single cell out of a million cells. Major advantages of the ELISPOT assay are its relatively easy performance, its potential for high throughput screening and no requirement for expensive instruments.