Acute Hepatic Failure in Dogs. Acute hepatic failure is a condition characterized by the sudden loss of 70 percent or more of the liver's function due to sudden, massive, hepatic necrosis (tissue death in the liver).
Key Points. Secondary (acquired) immunodeficiencies are much more common than primary (hereditary) immunodeficiencies. Primary immunodeficiencies can affect humoral immunity (most commonly), cellular immunity, both humoral and cellular immunity, phagocytic cells, or the complement system.
Indirect ELISA, conventional but efficient. Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labeled secondary antibody. The primary antibody is incubated with the antigen followed by the incubation with the secondary antibody.However, this may lead to nonspecific signals because of cross-reaction that the secondary antibody may bring about. 1. Micro-well plates are incubated with antigens, washed up and blocked with BSA. 2.he primary antibody is incubated with the antigen followed by the incubation with the secondary antibody. However, this may lead to nonspecific signals because of cross-reaction that the secondary antibody may bring about. 1. Micro-well plates are incubated with antigens, washed up and blocked with BSA. 2.