Signs and Symptoms. Because the signs of AS can be similar to those of other behavioral problems, it's best to let a doctor or other health professional evaluate a child's symptoms. Many kids are diagnosed with attention deficit hyperactivity disorder (ADHD) before a diagnosis of AS is made later.
Rubber begins to melt at approximately 180 degrees Celsius. At low temperatures, around 5 C to 6 C, there is a risk that rubber hardens because of crystallization. The optimum temperature for rubber is 20 C.
T m (primer) = Melting Temperature of the primers. T m (product) = Melting temperature of the product. 4. GC Content: The GC content (the number of G's and C's in the primer as a percentage of the total bases) of primer should be 40-60%.5. m of product is the melting temperature of the PCR product. 5. Primer Pair Tm Mismatch Calculation: The two primers of a primer pair should have closely matched melting temperatures for maximizing PCR product yield.