In March 2011, Campaign Chairman Jim Messina asked a group of 450 top donors to raise $350,000. During the second fundraising quarter of 2011 (the first of the campaign), the campaign raised a record amount of $86,000,000. As of May 3, 2012, Obama and his team have held 130 fundraisers.
A food vacuole is a membrane-enclosed cell vacuole with a digestive function, containing material taken up in by the process of phagocytosis. These are found in amoeba, protozoa, paramecium. In protozoa, whatever the mode of heterotrophic nutrition or diet, the food material is enclosed in food vacuoles, which are bounded by cell membrane. Digestive enzymes are poured into the newly formed vacuole from the surrounding cytoplasm.
Amoeba proteus has one contractile vacuole which slowly fills with water from the cytoplasm (diastole), then, while fusing with the cell membrane, quickly contracts (systole), releasing water to the outside by exocytosis. This process regulates the amount of water present in the cytoplasm of the amoeba.