-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
In November 1998, Melania met Donald at a Fashion Week party hosted by Paolo Zampolli, an Italian entrepreneur and founder of the ID Models management agency (he later worked with Trump on several real estate projects, and was renowned for using runway models to market high-end properties).