-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
But designer Sophie Theallet has flipped the script on those ubiquitous calls to boycott, announcing that instead it is she and her brand who will be boycotting Trump and his wife, Melania, refusing to lend her any of her designs.