-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
Enthusiastic âyesâ. Tommy Hilfiger: âI think Melania is a very beautiful woman and I think any designer should be proud to dress her.â (WWD, paywall) Yes, because I respect the office. Diane von Furstenberg: âMelania deserves the respect of any first lady before her.â (WWD)