-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
âMeet Melania Trump, a new model for first ladyâ. By Mary Jordan. Before she met Donald Trump at the Kit Kat Club in Manhattan and stepped into a glitzy world of limos and penthouses, diamonds and caviar, Melania Knauss lived quietly.