-toulouse-. Here's how I understand it. Adding the plasmid to the cells on ice makes the plasmid adhere to the cell wall. The heat shock does open the pores (made by the preparation of competent cells) and gets the plasmid to enter the cell.
Melania Trump wore a white dress by Roksanda Ilincic, a Serbian-born designer based in London, for her speech at the Republican National Convention. (Photo: Getty) On Monday night, the world was formally introduced to Republican nominee Donald Trumpâs wife, Melania.
âMy opinion is that more languages you speak, better it is, but but when you come to America, you speak English.â. Bio: Melania Trump (born Melanija Knavs on April 26, 1970 in Sevnica, Yugoslavia) is a Slovenian jewelry designer and former model. Melania grew up when Slovenia was a part of communist Yugoslavia. Her mother, Amalija Knavs, was a fashion designer.