You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
DNA Scissors: Introduction to Restriction EnzymesObjectives At the end of this activity, students should be able to1. Describe a typical restriction site as a 4- or 6-base-pair palindrome;2. Describe what a restriction enzyme does (recognize and cut at its restriction site);3.