You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Cutting and Splicing DNA Molecules. If you want to insert a piece of new DNA (foreign DNA) into an existing strand you'd have to cut the existing strand, somehow position the foreign DNA, and then join the strands. This joining is called ligation and can be done with the enzyme DNA ligase. Restriction enzymes are produced by bacteria and function to protect bacteria from foreign DNA (like viruses). Restriction enzymes work by degrading foreign DNA. Restriction enzymes recognize and cut the sugar phosphate backbone of the DNA molecule at specific sites on a strand of DNA. The restriction enzyme recognition site is determined by a specific sequence of nucleotides. Thousands of restriction enzymes have been isolated, although many share the same recognition sequence.