1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Melaina (The Black One) is the under-earth or chthonic aspect of the Greek Great Goddess, said to bring nightmares. Different Goddesses are called by Her name. Aphrodite Melainis represents a dark aspect of the Goddess of love as underworld Deity, though some say She is called Black because love-making often takes place at night.