You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Advertisement. Restriction enzymes are used to cut DNA at specific locations. Please choose the statement that best describes the role of restriction enzymes in genetic engineering. Southern blot analysis is used to identify DNA fragments that are carrying specific DNA sequences. Please choose the statement that best expresses the goal of Southern Blot analysis.