1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Taylor Lautner is an American Movie Actor, born on February 11, 1992 in Grand Rapids, Michigan, USA, his birth sign is the Aquarius and his Birth name Taylor Daniel Lautner. Taylor Lautner is an American actor, model and martial artist, he has been popular for his role as Jacob Black in the Twilight film franchise, the role which helped him to win several Teen Choice Awards and People's Choice Awards.