1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Addison Carver is a main character and a survivor of the initial zombie apocalypse in Z Nation. She is a member and current Second in Command of the Westward-bound survivor group. Background. Addison âAddyâ Grace Carver is a redheaded girl who seemingly defied the odds by surviving the zombie apocalypse. Before the world went to hell, people would describe her as sensitive, âwith the soul of an artist.â When push came to shove, she had the guts of a killer.