1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Sir Mordred. Arthurian Literary Character. Sir Mordred appears to have been an historical personage. Tradition makes him the youngest son of Queen Morgause of Orkney. He was raised as a son of her husband, King Lot, but - due to an unfortunate and uninformed encounter - his real father was his own uncle, King Arthur.