You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Restriction enzymes recognize and cut at specific places along the DNA molecule called. restriction sites. Each different restriction enzyme (and there are hundreds, made by many. different bacteria) has its own type of site. In general, a restriction site is a 4- or 6-base-pair. sequence that is a palindrome.