1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
hairdresser - someone who cuts or beautifies hair. hairstylist, styler, stylist. barber - a hairdresser who cuts hair and shaves beards as a trade. coiffeur - a man hairdresser. coiffeuse - a woman hairdresser. artisan, journeyman, artificer, craftsman - a skilled worker who practices some trade or handicraft.