M. luteus can be found in many places such as the human skin, water, dust, and soil. Micrococcus is generally thought of as harmless bacterium, but there have been rare cases of Micrococcus infections in people with compromised immune systems, as occurs with HIV patients.
Therefore when used in gel electrophoresis, markers effectively provide a logarithmic scale by which to estimate the size of the other fragments (providing the fragment sizes of the marker are known). Protein, DNA, and RNA markers with pre-determined fragment sizes and concentrations are commercially available.
1 Bio 210A DNA Gel Electrophoresis Purpose â¢ To learn the scientific basis of DNA gel electrophoresis. â¢ To learn how to pour agarose gels. â¢ To separate DNA fragments by gel electrophoresis. â¢ To analyze the size DNA fragments using a standard molecular weight marker. Introduction Agarose gel electrophoresis is widely used in biotechnology for analysis of DNA and RNA. This technique separates molecules based upon charge, size and shape.