1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Meleni Rodney. |} Meleni Rodney (born 27 June 1998) is a Grenadian sprinter who specialises in the 400 metres. She participated at the Summer Youth Olympics in 2014 and received a bronze medal. In that same year she was named athlete of the year along with Kirani James.