You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Thus, if you want to move a piece of DNA from one plasmid to another, you can use restriction enzymes to cut it out of one plasmid, then use the same restriction enzymes to create a gap in the destination plasmid, purify the desired segments, and mix the results.