1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Mordred is in love with a druid named Kara who has killed many in revenge for Arthur killing people with magic. Mordred asks Merlin not to tell Arthur about her, but he does and when Arthur finds her and finds out she is injured, he offers to help her but she tries to kill Arthur.rthur was born in Camelot to Uther and Ygraine Pendragon with help of magic by the sorceress and priestess, Nimueh. Ygraine was barren and incapable of giving birth, so Uther, who was desperate for her to conceive an heir, went to Nimueh and persuaded her to use her magic to help Ygraine.