1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Jean Bodel (c. 1165 â c. 1210), was an Old French poet who wrote a number of chansons de geste as well as many fabliaux. He lived in Arras. Bodel wrote the Chanson de Saisnes, about the war of King Charlemagne with the Saxons and their leader Widukind, whom Bodel calls Guiteclin. He also wrote a miracle play called the Jeu de Saint Nicolas, which was probably first performed in Arras on 5 December 1200.