1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
âOlympe Maxime to Rubeus Hagrid after he states that she is half-giant. Madame Olympe Maxime (fl. 1994-2010) is a French half-giant witch and was the headmistress of Beauxbatons Academy of Magic. Maxime's preferred mode of transportation is to travel with her students in a powder-blue carriage drawn by twelve winged Abraxan horses, which are bred at Beauxbatons Academy of Magic.