You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
first cut out the insulin gene from human DNA using chemical scissors know as restriction enzymes. Use restriction enzymes to open up plasmid and insert. Use the enzyme called ligase to seal up the plasmid. insert plasmid bake into bacterium (usually by giving the bacteria an electric shock)