You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Restriction enzymes are used to cut up DNA into fragments with 'sticky ends'. It allows for the gene of interest to be isolated. A plasmid can then also be cut with the same r â¦ estriction enzyme and the sticky ends are spliced together with DNA ligase.estriction enzymes are used to cut up DNA into fragments with 'sticky ends'. It allows for the gene of interest to be isolated. A plasmid can then also be cut with the same r â¦ estriction enzyme and the sticky ends are spliced together with DNA ligase.