1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Sejong the Great (Korean pronunciation: [se.dÊoÅ]; 15 May 1397 - 8 April 1450) was the fourth king of Joseon-dynasty Korea. He was the third son of King Taejong and Queen consort Min. He was designated as heir-apparent, Crown Prince, after his older brother Jae was stripped of his title.