You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
When a restriction enzyme cleaves a restriction site, the reaction creates highly reactive sticky ends on the broken DNA. This is useful to the biotechnologist! By cutting open vector DNA with the same with restriction enzymes used to cleave the target DNA, complementary sticky ends are created.