1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Billie Paul Piper (born Lianne Paul Piper, 22 September 1982, in Swindon, Wiltshire) is an English singer and actress. She began her career in the late 1990s as a pop singer. Later on, she moved to acting. Her most famous role is as Rose Tyler, a companion to the Doctor in the television series Doctor Who.