1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Sonequa Martin-Green was born on March 21, 1985 in Russellville, Alabama, USA. She is an actress and writer, known for The Walking Dead (2010), Toe to Toe (2009) and Shockwave Darkside (2014). She has been married to Kenric Green since December 4, 2010. They have one child.